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Non-aqueous capillary electrophoresis for separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its medicinal preparations.
Biomed Chromatogr. 2005 Nov; 19(9):696-702.BC

Abstract

A non-aqueous capillary electrophoresis method has been developed for the separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its preparation for the first time. Optimum separation of the analytes was obtained on a 47 cm x 75 microm i.d. fused-silica capillary using a non-aqueous buffer system of 60 mM sodium cholate, 20 mM ammonium acetate, 20% acetonitrile and 3% acetic acid at 20 kV and 292 K, respectively. The relative standard deviations (RSDs) of the migration times and the peak heights of the three analytes were in the range of 0.23-0.28 and 2.12-2.60%, respectively. Detection limits of fraxin, esculin and esculetin were 0.1557, 0.4073 and 0.5382 microg/mL, respectively. In the tested concentration range, good linear relationships (correlation coefficients 0.9995 for fraxin, 0.9999 for esculin and 0.9992 for esculetin) between peak heights and concentrations of the analytes were observed. This method has been successfully applied to simultaneous determination of the three bioactive components with the recoveries from 90.2 to 109.2% in the five samples.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15828063

Citation

Li, Cunhong, et al. "Non-aqueous Capillary Electrophoresis for Separation and Simultaneous Determination of Fraxin, Esculin and Esculetin in Cortex Fraxini and Its Medicinal Preparations." Biomedical Chromatography : BMC, vol. 19, no. 9, 2005, pp. 696-702.
Li C, Chen A, Chen X, et al. Non-aqueous capillary electrophoresis for separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its medicinal preparations. Biomed Chromatogr. 2005;19(9):696-702.
Li, C., Chen, A., Chen, X., Ma, X., Chen, X., & Hu, Z. (2005). Non-aqueous capillary electrophoresis for separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its medicinal preparations. Biomedical Chromatography : BMC, 19(9), 696-702.
Li C, et al. Non-aqueous Capillary Electrophoresis for Separation and Simultaneous Determination of Fraxin, Esculin and Esculetin in Cortex Fraxini and Its Medicinal Preparations. Biomed Chromatogr. 2005;19(9):696-702. PubMed PMID: 15828063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-aqueous capillary electrophoresis for separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its medicinal preparations. AU - Li,Cunhong, AU - Chen,Anjia, AU - Chen,Xiaofeng, AU - Ma,Xiao, AU - Chen,Xingguo, AU - Hu,Zhide, PY - 2005/4/14/pubmed PY - 2005/12/17/medline PY - 2005/4/14/entrez SP - 696 EP - 702 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 19 IS - 9 N2 - A non-aqueous capillary electrophoresis method has been developed for the separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its preparation for the first time. Optimum separation of the analytes was obtained on a 47 cm x 75 microm i.d. fused-silica capillary using a non-aqueous buffer system of 60 mM sodium cholate, 20 mM ammonium acetate, 20% acetonitrile and 3% acetic acid at 20 kV and 292 K, respectively. The relative standard deviations (RSDs) of the migration times and the peak heights of the three analytes were in the range of 0.23-0.28 and 2.12-2.60%, respectively. Detection limits of fraxin, esculin and esculetin were 0.1557, 0.4073 and 0.5382 microg/mL, respectively. In the tested concentration range, good linear relationships (correlation coefficients 0.9995 for fraxin, 0.9999 for esculin and 0.9992 for esculetin) between peak heights and concentrations of the analytes were observed. This method has been successfully applied to simultaneous determination of the three bioactive components with the recoveries from 90.2 to 109.2% in the five samples. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15828063/Non_aqueous_capillary_electrophoresis_for_separation_and_simultaneous_determination_of_fraxin_esculin_and_esculetin_in_Cortex_fraxini_and_its_medicinal_preparations_ L2 - https://doi.org/10.1002/bmc.497 DB - PRIME DP - Unbound Medicine ER -