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[Study of the factors effecting surface-enhanced Raman scattering reporter-labeled immunogold colloids].
Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Dec; 24(12):1575-8.GP

Abstract

The applications of the high sensitivity of Surface-Enhanced Raman Spectroscopy (SERS) in labeled immunoassay have proved to be very useful. In the sandwich structure of "immobile antibody-antigen-labeled antibody", the authors prepared the labeled antibody by attaching gold nanoparticles, which was first labeled with molecule that had SERS signal, to the antibody. In this article, colloids were labeled with Raman reporter thiophenol through S-Au bond on gold and formed surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Then, goat anti-rat antibody was adsorbed onto the reporter-labeled gold colloids and formed reporter-labeled immunogold colloids. This paper studied factors affecting FT-SERS reporter-labeled immunogold colloids such as the sizes of gold particles, the amount of thiophenol in gold colloids and processing time, and whether goat anti-rat antibody would influence the SERS of reporter-labeled gold colloids.

Authors+Show Affiliations

Li SJ
Department of Chemistry, Suzhou University, Suzhou 215006, China.
Qiu LQ
No affiliation info available
Cao PG
No affiliation info available
Gu RA
No affiliation info available

MeSH

AnimalsAntibodies, Anti-IdiotypicGoldGold ColloidImmunoassayMetal NanoparticlesPhenolsRatsSpectrum Analysis, RamanSulfhydryl CompoundsSurface Plasmon Resonance

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15828331

Citation

Li, Shu-jin, et al. "[Study of the Factors Effecting Surface-enhanced Raman Scattering Reporter-labeled Immunogold Colloids]." Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu, vol. 24, no. 12, 2004, pp. 1575-8.
Li SJ, Qiu LQ, Cao PG, et al. [Study of the factors effecting surface-enhanced Raman scattering reporter-labeled immunogold colloids]. Guang Pu Xue Yu Guang Pu Fen Xi. 2004;24(12):1575-8.
Li, S. J., Qiu, L. Q., Cao, P. G., & Gu, R. A. (2004). [Study of the factors effecting surface-enhanced Raman scattering reporter-labeled immunogold colloids]. Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu, 24(12), 1575-8.
Li SJ, et al. [Study of the Factors Effecting Surface-enhanced Raman Scattering Reporter-labeled Immunogold Colloids]. Guang Pu Xue Yu Guang Pu Fen Xi. 2004;24(12):1575-8. PubMed PMID: 15828331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study of the factors effecting surface-enhanced Raman scattering reporter-labeled immunogold colloids]. AU - Li,Shu-jin, AU - Qiu,Li-qun, AU - Cao,Pei-geng, AU - Gu,Ren-ao, PY - 2005/4/15/pubmed PY - 2010/6/4/medline PY - 2005/4/15/entrez SP - 1575 EP - 8 JF - Guang pu xue yu guang pu fen xi = Guang pu JO - Guang Pu Xue Yu Guang Pu Fen Xi VL - 24 IS - 12 N2 - The applications of the high sensitivity of Surface-Enhanced Raman Spectroscopy (SERS) in labeled immunoassay have proved to be very useful. In the sandwich structure of "immobile antibody-antigen-labeled antibody", the authors prepared the labeled antibody by attaching gold nanoparticles, which was first labeled with molecule that had SERS signal, to the antibody. In this article, colloids were labeled with Raman reporter thiophenol through S-Au bond on gold and formed surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Then, goat anti-rat antibody was adsorbed onto the reporter-labeled gold colloids and formed reporter-labeled immunogold colloids. This paper studied factors affecting FT-SERS reporter-labeled immunogold colloids such as the sizes of gold particles, the amount of thiophenol in gold colloids and processing time, and whether goat anti-rat antibody would influence the SERS of reporter-labeled gold colloids. SN - 1000-0593 UR - https://www.unboundmedicine.com/medline/citation/15828331/[Study_of_the_factors_effecting_surface_enhanced_Raman_scattering_reporter_labeled_immunogold_colloids]_ DB - PRIME DP - Unbound Medicine ER -