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Altered cytokine expression of peripheral blood lymphocytes in polymyositis and dermatomyositis.
Ann Rheum Dis. 2005 Oct; 64(10):1485-9.AR

Abstract

OBJECTIVE

To investigate the intracellular and soluble cytokine levels and T cell subsets in peripheral blood of patients with active and inactive polymyositis and dermatomyositis.

METHODS

The frequencies of T and B lymphocytes, T helper (Th), and T cytotoxic (Tc) cells and of interferon gamma (IFNgamma), interleukin (IL)4, and IL10 expression of CD4+ or CD8+ cells were determined by flow cytometry. The concentrations of soluble cytokines were measured with commercial enzyme linked immunosorbent assays.

RESULTS

In active dermatomyositis there was a decreased percentage of T (CD3+) lymphocytes and Tc (CD8+) lymphocytes, decreased IFNgamma expression of CD4+ and CD8+ cells, but an increase in B and IL4 producing CD4+ lymphocyte frequencies. These prominent changes disappeared in the inactive stage of the disease. In polymyositis no significant change in these lymphocyte subsets or in intracellular cytokine expression could be detected in either the active or the inactive form. The frequency of IL4+/IFNgamma+ Th cells was calculated and a significantly increased Th2/Th1 frequency was found in active dermatomyositis, and a decreased frequency in inactive dermatomyositis, compared with the control population.

CONCLUSIONS

There appears to be a difference between polymyositis and dermatomyositis in the level of peripheral blood lymphocytes and their intracellular cytokine content. These findings provide further evidence for a difference in the pathogenesis of polymyositis and dermatomyositis.

Authors+Show Affiliations

Third Department of Internal Medicine, University of Debrecen, Medical and Health Centre, Debrecen, Hungary. deb4@freemail.huNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15829578

Citation

Aleksza, M, et al. "Altered Cytokine Expression of Peripheral Blood Lymphocytes in Polymyositis and Dermatomyositis." Annals of the Rheumatic Diseases, vol. 64, no. 10, 2005, pp. 1485-9.
Aleksza M, Szegedi A, Antal-Szalmás P, et al. Altered cytokine expression of peripheral blood lymphocytes in polymyositis and dermatomyositis. Ann Rheum Dis. 2005;64(10):1485-9.
Aleksza, M., Szegedi, A., Antal-Szalmás, P., Irinyi, B., Gergely, L., Ponyi, A., Hunyadi, J., Sipka, S., Zeher, M., Szegedi, G., & Dankó, K. (2005). Altered cytokine expression of peripheral blood lymphocytes in polymyositis and dermatomyositis. Annals of the Rheumatic Diseases, 64(10), 1485-9.
Aleksza M, et al. Altered Cytokine Expression of Peripheral Blood Lymphocytes in Polymyositis and Dermatomyositis. Ann Rheum Dis. 2005;64(10):1485-9. PubMed PMID: 15829578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered cytokine expression of peripheral blood lymphocytes in polymyositis and dermatomyositis. AU - Aleksza,M, AU - Szegedi,A, AU - Antal-Szalmás,P, AU - Irinyi,B, AU - Gergely,L, AU - Ponyi,A, AU - Hunyadi,J, AU - Sipka,S, AU - Zeher,M, AU - Szegedi,G, AU - Dankó,K, Y1 - 2005/04/13/ PY - 2005/4/15/pubmed PY - 2005/11/4/medline PY - 2005/4/15/entrez SP - 1485 EP - 9 JF - Annals of the rheumatic diseases JO - Ann Rheum Dis VL - 64 IS - 10 N2 - OBJECTIVE: To investigate the intracellular and soluble cytokine levels and T cell subsets in peripheral blood of patients with active and inactive polymyositis and dermatomyositis. METHODS: The frequencies of T and B lymphocytes, T helper (Th), and T cytotoxic (Tc) cells and of interferon gamma (IFNgamma), interleukin (IL)4, and IL10 expression of CD4+ or CD8+ cells were determined by flow cytometry. The concentrations of soluble cytokines were measured with commercial enzyme linked immunosorbent assays. RESULTS: In active dermatomyositis there was a decreased percentage of T (CD3+) lymphocytes and Tc (CD8+) lymphocytes, decreased IFNgamma expression of CD4+ and CD8+ cells, but an increase in B and IL4 producing CD4+ lymphocyte frequencies. These prominent changes disappeared in the inactive stage of the disease. In polymyositis no significant change in these lymphocyte subsets or in intracellular cytokine expression could be detected in either the active or the inactive form. The frequency of IL4+/IFNgamma+ Th cells was calculated and a significantly increased Th2/Th1 frequency was found in active dermatomyositis, and a decreased frequency in inactive dermatomyositis, compared with the control population. CONCLUSIONS: There appears to be a difference between polymyositis and dermatomyositis in the level of peripheral blood lymphocytes and their intracellular cytokine content. These findings provide further evidence for a difference in the pathogenesis of polymyositis and dermatomyositis. SN - 0003-4967 UR - https://www.unboundmedicine.com/medline/citation/15829578/Altered_cytokine_expression_of_peripheral_blood_lymphocytes_in_polymyositis_and_dermatomyositis_ L2 - https://ard.bmj.com/lookup/pmidlookup?view=long&pmid=15829578 DB - PRIME DP - Unbound Medicine ER -