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Transgenic mice that express Cre recombinase in hypertrophic chondrocytes.
Genesis. 2005 May; 42(1):33-6.G

Abstract

In order to investigate the physiological control of hypertrophic chondrocytes which present the terminally differentiated form of chondrocytes, we generated a mouse line expressing the Cre recombinase under the control of the mouse type X collagen (Col10a1) promoter. In situ hybridization analysis demonstrated the expression of Col10a1-Cre transgene in hypertrophic chondrocytes of femur at postnatal day 2 (P2). In order to test the excision activity of the Cre recombinase, the Col10a1-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4co/co) and the reporter line ROSA26. Multiple tissue PCR of Col10a1-Cre;Smad4co/+ mice revealed the restricted Cre activity in tissues containing hypertrophic chondrocytes. LacZ staining revealed that the Cre activity was observed in the cartilage primordia of ribs at E14.5 and only detected in the lower hypertrophic region of ribs at P1. These data suggest that the Col10a1-Cre mouse line described here could be used to achieve conditional gene targeting in hypertrophic chondrocytes.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Fengtai, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15830383

Citation

Yang, Guan, et al. "Transgenic Mice That Express Cre Recombinase in Hypertrophic Chondrocytes." Genesis (New York, N.Y. : 2000), vol. 42, no. 1, 2005, pp. 33-6.
Yang G, Cui F, Hou N, et al. Transgenic mice that express Cre recombinase in hypertrophic chondrocytes. Genesis. 2005;42(1):33-6.
Yang, G., Cui, F., Hou, N., Cheng, X., Zhang, J., Wang, Y., Jiang, N., Gao, X., & Yang, X. (2005). Transgenic mice that express Cre recombinase in hypertrophic chondrocytes. Genesis (New York, N.Y. : 2000), 42(1), 33-6.
Yang G, et al. Transgenic Mice That Express Cre Recombinase in Hypertrophic Chondrocytes. Genesis. 2005;42(1):33-6. PubMed PMID: 15830383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transgenic mice that express Cre recombinase in hypertrophic chondrocytes. AU - Yang,Guan, AU - Cui,Fang, AU - Hou,Ning, AU - Cheng,Xuan, AU - Zhang,Jishuai, AU - Wang,Youliang, AU - Jiang,Nan, AU - Gao,Xiang, AU - Yang,Xiao, PY - 2005/4/15/pubmed PY - 2005/8/17/medline PY - 2005/4/15/entrez SP - 33 EP - 6 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 42 IS - 1 N2 - In order to investigate the physiological control of hypertrophic chondrocytes which present the terminally differentiated form of chondrocytes, we generated a mouse line expressing the Cre recombinase under the control of the mouse type X collagen (Col10a1) promoter. In situ hybridization analysis demonstrated the expression of Col10a1-Cre transgene in hypertrophic chondrocytes of femur at postnatal day 2 (P2). In order to test the excision activity of the Cre recombinase, the Col10a1-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4co/co) and the reporter line ROSA26. Multiple tissue PCR of Col10a1-Cre;Smad4co/+ mice revealed the restricted Cre activity in tissues containing hypertrophic chondrocytes. LacZ staining revealed that the Cre activity was observed in the cartilage primordia of ribs at E14.5 and only detected in the lower hypertrophic region of ribs at P1. These data suggest that the Col10a1-Cre mouse line described here could be used to achieve conditional gene targeting in hypertrophic chondrocytes. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15830383/Transgenic_mice_that_express_Cre_recombinase_in_hypertrophic_chondrocytes_ L2 - https://doi.org/10.1002/gene.20120 DB - PRIME DP - Unbound Medicine ER -