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1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine.
J Forensic Sci. 2005 Jan; 50(1):81-6.JF

Abstract

A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH.

Authors+Show Affiliations

Del Signore AG
Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.
McGregor M
No affiliation info available
Cho BP
No affiliation info available

MeSH

4-ButyrolactoneCalibrationForensic MedicineHumansHydrogen-Ion ConcentrationHydrolysisHydroxybutyratesMagnetic Resonance SpectroscopySensitivity and SpecificitySolventsSpecimen Handling

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15831000

Citation

Del Signore, Anthony G., et al. "1H NMR Analysis of GHB and GBL: Further Findings On the Interconversion and a Preliminary Report On the Analysis of GHB in Serum and Urine." Journal of Forensic Sciences, vol. 50, no. 1, 2005, pp. 81-6.
Del Signore AG, McGregor M, Cho BP. 1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine. J Forensic Sci. 2005;50(1):81-6.
Del Signore, A. G., McGregor, M., & Cho, B. P. (2005). 1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine. Journal of Forensic Sciences, 50(1), 81-6.
Del Signore AG, McGregor M, Cho BP. 1H NMR Analysis of GHB and GBL: Further Findings On the Interconversion and a Preliminary Report On the Analysis of GHB in Serum and Urine. J Forensic Sci. 2005;50(1):81-6. PubMed PMID: 15831000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine. AU - Del Signore,Anthony G, AU - McGregor,Michael, AU - Cho,Bongsup P, PY - 2005/4/16/pubmed PY - 2005/5/6/medline PY - 2005/4/16/entrez SP - 81 EP - 6 JF - Journal of forensic sciences JO - J Forensic Sci VL - 50 IS - 1 N2 - A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/15831000/1H_NMR_analysis_of_GHB_and_GBL:_further_findings_on_the_interconversion_and_a_preliminary_report_on_the_analysis_of_GHB_in_serum_and_urine_ DB - PRIME DP - Unbound Medicine ER -