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1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine.
J Forensic Sci. 2005 Jan; 50(1):81-6.JF

Abstract

A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH.

Authors+Show Affiliations

Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15831000

Citation

Del Signore, Anthony G., et al. "1H NMR Analysis of GHB and GBL: Further Findings On the Interconversion and a Preliminary Report On the Analysis of GHB in Serum and Urine." Journal of Forensic Sciences, vol. 50, no. 1, 2005, pp. 81-6.
Del Signore AG, McGregor M, Cho BP. 1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine. J Forensic Sci. 2005;50(1):81-6.
Del Signore, A. G., McGregor, M., & Cho, B. P. (2005). 1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine. Journal of Forensic Sciences, 50(1), 81-6.
Del Signore AG, McGregor M, Cho BP. 1H NMR Analysis of GHB and GBL: Further Findings On the Interconversion and a Preliminary Report On the Analysis of GHB in Serum and Urine. J Forensic Sci. 2005;50(1):81-6. PubMed PMID: 15831000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine. AU - Del Signore,Anthony G, AU - McGregor,Michael, AU - Cho,Bongsup P, PY - 2005/4/16/pubmed PY - 2005/5/6/medline PY - 2005/4/16/entrez SP - 81 EP - 6 JF - Journal of forensic sciences JO - J Forensic Sci VL - 50 IS - 1 N2 - A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/15831000/1H_NMR_analysis_of_GHB_and_GBL:_further_findings_on_the_interconversion_and_a_preliminary_report_on_the_analysis_of_GHB_in_serum_and_urine_ DB - PRIME DP - Unbound Medicine ER -