Tags

Type your tag names separated by a space and hit enter

alpha-galf I -->6-alpha-mannopyranoside side chains in Paracoccidioides brasiliensis cell wall are shared by members of the Onygenales, but not by galactomannans of other fungal genera.
Med Mycol. 2005 Mar; 43(2):153-9.MM

Abstract

The water-soluble polysaccharide fraction of the cell wall alkali extract (F1SS) from the mycelial phase of the dimorphic fungus Paracoccidioides brasiliensis is compared with F1SS polysaccharides obtained from the Onygenalean mycelial fungi Ascocalvatia alveolata, Onygena equina and Aphanoascus terreus. These polymers were exclusively composed of mannose and galactose. Data from methylation and NMR analyses reveal that F1SS polysaccharides from the four fungi contain the same residues although in different proportions: [-->2,6)-alpha-D-Manp-(1 -->]; [2)-alpha-D-Manp-(1 -->]; [ -->6)-alpha-D-Manp-(1 -->]; and [alpha-D-Galf-(1 -->]. In P. brasiliensis, the repeating unit of the polysaccharide consists of a backbone of [(1 -->6)-alpha-D-Manp] substituted at the 0-2 position by the disaccharide [alpha-D-Galf-(1 -->6)-alpha-D-Manp-(1 -->], while the remaining 0-2 positions are substituted by single residues of mannose or short chains of (1 -->2)-mannose. The other species had a lower proportion of galactofuranose-containing side chains and higher proportion of mannose-containing side chains. The similarities found among the F1SS polysaccharides from P. brasiliensis and the Onygenalean A. alveolata, A. terreus and O. equina, reveal the close relatedness of all these fungi, show differences with polysaccharides from other fungal genera and agree with the molecular evidence provided in the scientific literature for the placement of P. brasiliensis within the Onygenales.

Authors+Show Affiliations

San-Blas G
Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela. sanblasg@ivic.ve
Prieto A
No affiliation info available
Bernabé M
No affiliation info available
Ahrazem O
No affiliation info available
Moreno B
No affiliation info available
Leal JA
No affiliation info available

MeSH

Carbohydrate SequenceCell WallMagnetic Resonance SpectroscopyMannansMolecular Sequence DataMyceliumOnygenalesParacoccidioides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15832558

Citation

San-Blas, G, et al. "Alpha-galf I -->6-alpha-mannopyranoside Side Chains in Paracoccidioides Brasiliensis Cell Wall Are Shared By Members of the Onygenales, but Not By Galactomannans of Other Fungal Genera." Medical Mycology, vol. 43, no. 2, 2005, pp. 153-9.
San-Blas G, Prieto A, Bernabé M, et al. Alpha-galf I -->6-alpha-mannopyranoside side chains in Paracoccidioides brasiliensis cell wall are shared by members of the Onygenales, but not by galactomannans of other fungal genera. Med Mycol. 2005;43(2):153-9.
San-Blas, G., Prieto, A., Bernabé, M., Ahrazem, O., Moreno, B., & Leal, J. A. (2005). Alpha-galf I -->6-alpha-mannopyranoside side chains in Paracoccidioides brasiliensis cell wall are shared by members of the Onygenales, but not by galactomannans of other fungal genera. Medical Mycology, 43(2), 153-9.
San-Blas G, et al. Alpha-galf I -->6-alpha-mannopyranoside Side Chains in Paracoccidioides Brasiliensis Cell Wall Are Shared By Members of the Onygenales, but Not By Galactomannans of Other Fungal Genera. Med Mycol. 2005;43(2):153-9. PubMed PMID: 15832558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - alpha-galf I -->6-alpha-mannopyranoside side chains in Paracoccidioides brasiliensis cell wall are shared by members of the Onygenales, but not by galactomannans of other fungal genera. AU - San-Blas,G, AU - Prieto,A, AU - Bernabé,M, AU - Ahrazem,O, AU - Moreno,B, AU - Leal,J A, PY - 2005/4/19/pubmed PY - 2005/6/25/medline PY - 2005/4/19/entrez SP - 153 EP - 9 JF - Medical mycology JO - Med Mycol VL - 43 IS - 2 N2 - The water-soluble polysaccharide fraction of the cell wall alkali extract (F1SS) from the mycelial phase of the dimorphic fungus Paracoccidioides brasiliensis is compared with F1SS polysaccharides obtained from the Onygenalean mycelial fungi Ascocalvatia alveolata, Onygena equina and Aphanoascus terreus. These polymers were exclusively composed of mannose and galactose. Data from methylation and NMR analyses reveal that F1SS polysaccharides from the four fungi contain the same residues although in different proportions: [-->2,6)-alpha-D-Manp-(1 -->]; [2)-alpha-D-Manp-(1 -->]; [ -->6)-alpha-D-Manp-(1 -->]; and [alpha-D-Galf-(1 -->]. In P. brasiliensis, the repeating unit of the polysaccharide consists of a backbone of [(1 -->6)-alpha-D-Manp] substituted at the 0-2 position by the disaccharide [alpha-D-Galf-(1 -->6)-alpha-D-Manp-(1 -->], while the remaining 0-2 positions are substituted by single residues of mannose or short chains of (1 -->2)-mannose. The other species had a lower proportion of galactofuranose-containing side chains and higher proportion of mannose-containing side chains. The similarities found among the F1SS polysaccharides from P. brasiliensis and the Onygenalean A. alveolata, A. terreus and O. equina, reveal the close relatedness of all these fungi, show differences with polysaccharides from other fungal genera and agree with the molecular evidence provided in the scientific literature for the placement of P. brasiliensis within the Onygenales. SN - 1369-3786 UR - https://www.unboundmedicine.com/medline/citation/15832558/alpha_galf_I___>6_alpha_mannopyranoside_side_chains_in_Paracoccidioides_brasiliensis_cell_wall_are_shared_by_members_of_the_Onygenales_but_not_by_galactomannans_of_other_fungal_genera_ DB - PRIME DP - Unbound Medicine ER -