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Kinetics and thermodynamics of ethanol production by a thermotolerant mutant of Saccharomyces cerevisiae in a microprocessor-controlled bioreactor.
Lett Appl Microbiol. 2005; 40(5):316-21.LA

Abstract

AIMS

The present investigation deals with the development of thermotolerant mutant strain of yeast for studying enhanced productivity of ethanol from molasses in a fully controlled bioreactor.

METHODS AND RESULTS

The parental culture of Saccharomyces cerevisiae ATCC 26602 was mutated using UV treatment. A single thermotolerant mutant was isolated after extensive screening and optimization, and grown on molasses medium in liquid cultures. The mutant was 1.45-fold improved than its wild parent with respect to ethanol productivity (7.2 g l-1 h-1), product yield (0.44 g ethanol g-1 substrate utilized) and specific ethanol yield (19.0 g ethanol g-1 cells). The improved ethanol productivity was directly correlated with titres of intracellular and extracellular invertase activities. The mutant supported higher volumetric and product yield of ethanol, significantly (P<or=0.05) higher than the parental and other strains. The mutated cultures produced 1.8- and 2.6-fold more extracellular and intracellular invertase productivity, respectively, than that produced by its wild parent at 40 degrees C. Thermodynamic studies revealed that the cell system exerted protection against thermal inactivation during formation of products.

CONCLUSIONS

A mutant derivative of Sacchromyces cerevisiae with improved productivity of ethanol and invertases has been obtained, which showed concomitant improvement in thermostability of endogenous metabolism for formation of both ethanol and invertases.

SIGNIFICANCE AND IMPACT OF THE STUDY

The results of the present study are of commercial value as the mutant can be used for ethanol production in parts of Pakistan where the temperature may go up to 40 degrees C in April. Ethanol product yield coefficient and volumetric productivity, revealed the hyper-productivity of ethanol from molasses at 40 degrees C, which is not appropriate for wild organism.

Authors+Show Affiliations

Rajoka MI
Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan. mirajoka@nibge.org
Ferhan M
No affiliation info available
Khalid AM
No affiliation info available

MeSH

BioreactorsCulture MediaEthanolFermentationIndustrial MicrobiologyMolassesMutationSaccharomyces cerevisiaeSubstrate SpecificityTemperaturebeta-Fructofuranosidase

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15836732

Citation

Rajoka, M I., et al. "Kinetics and Thermodynamics of Ethanol Production By a Thermotolerant Mutant of Saccharomyces Cerevisiae in a Microprocessor-controlled Bioreactor." Letters in Applied Microbiology, vol. 40, no. 5, 2005, pp. 316-21.
Rajoka MI, Ferhan M, Khalid AM. Kinetics and thermodynamics of ethanol production by a thermotolerant mutant of Saccharomyces cerevisiae in a microprocessor-controlled bioreactor. Lett Appl Microbiol. 2005;40(5):316-21.
Rajoka, M. I., Ferhan, M., & Khalid, A. M. (2005). Kinetics and thermodynamics of ethanol production by a thermotolerant mutant of Saccharomyces cerevisiae in a microprocessor-controlled bioreactor. Letters in Applied Microbiology, 40(5), 316-21.
Rajoka MI, Ferhan M, Khalid AM. Kinetics and Thermodynamics of Ethanol Production By a Thermotolerant Mutant of Saccharomyces Cerevisiae in a Microprocessor-controlled Bioreactor. Lett Appl Microbiol. 2005;40(5):316-21. PubMed PMID: 15836732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetics and thermodynamics of ethanol production by a thermotolerant mutant of Saccharomyces cerevisiae in a microprocessor-controlled bioreactor. AU - Rajoka,M I, AU - Ferhan,M, AU - Khalid,A M, PY - 2005/4/20/pubmed PY - 2005/10/19/medline PY - 2005/4/20/entrez SP - 316 EP - 21 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 40 IS - 5 N2 - AIMS: The present investigation deals with the development of thermotolerant mutant strain of yeast for studying enhanced productivity of ethanol from molasses in a fully controlled bioreactor. METHODS AND RESULTS: The parental culture of Saccharomyces cerevisiae ATCC 26602 was mutated using UV treatment. A single thermotolerant mutant was isolated after extensive screening and optimization, and grown on molasses medium in liquid cultures. The mutant was 1.45-fold improved than its wild parent with respect to ethanol productivity (7.2 g l-1 h-1), product yield (0.44 g ethanol g-1 substrate utilized) and specific ethanol yield (19.0 g ethanol g-1 cells). The improved ethanol productivity was directly correlated with titres of intracellular and extracellular invertase activities. The mutant supported higher volumetric and product yield of ethanol, significantly (P<or=0.05) higher than the parental and other strains. The mutated cultures produced 1.8- and 2.6-fold more extracellular and intracellular invertase productivity, respectively, than that produced by its wild parent at 40 degrees C. Thermodynamic studies revealed that the cell system exerted protection against thermal inactivation during formation of products. CONCLUSIONS: A mutant derivative of Sacchromyces cerevisiae with improved productivity of ethanol and invertases has been obtained, which showed concomitant improvement in thermostability of endogenous metabolism for formation of both ethanol and invertases. SIGNIFICANCE AND IMPACT OF THE STUDY: The results of the present study are of commercial value as the mutant can be used for ethanol production in parts of Pakistan where the temperature may go up to 40 degrees C in April. Ethanol product yield coefficient and volumetric productivity, revealed the hyper-productivity of ethanol from molasses at 40 degrees C, which is not appropriate for wild organism. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/15836732/Kinetics_and_thermodynamics_of_ethanol_production_by_a_thermotolerant_mutant_of_Saccharomyces_cerevisiae_in_a_microprocessor_controlled_bioreactor_ DB - PRIME DP - Unbound Medicine ER -