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Mitochondrial haplotype diversity in the tortoise species Testudo graeca from North Africa and the Middle East.
BMC Evol Biol 2005; 5:29BE

Abstract

BACKGROUND

To help conservation programs of the endangered spur-thighed tortoise and to gain better insight into its systematics, genetic variation and evolution in the tortoise species Testudo graeca (Testudines: Testudinidae) was investigated by sequence analysis of a 394-nucleotide fragment of the mitochondrial 12S rRNA gene for 158 tortoise specimens belonging to the subspecies Testudo graeca graeca, Testudo graeca ibera, Testudo graeca terrestris, and a newly recognized subspecies Testudo graeca whitei. A 411-nucleotide fragment of the mitochondrial D-loop was additionally sequenced for a subset of 22 T. graeca, chosen because of their 12S gene haplotype and/or geographical origin.

RESULTS

Haplotype networks generated by maximum-likelihood and neighbor-joining analyses of both the separate and the combined sequence data sets suggested the existence of two main clades of Testudo graeca, comprising Testudo graeca from northern Africa and Testudo graeca from the Turkey and the Middle East, respectively.

CONCLUSION

Mitochondrial DNA haplotyping suggests that the tortoise subspecies of T. g. graeca and T. g. ibera are genetically distinct, with a calculated divergence time in the early or middle Pleistocene. Other proposed subspecies could not clearly be recognized based upon their mt haplotypes and phylogenetic position, and were either part of the T. g. graeca or of the T. g. ibera clade, suggesting that genetic evidence for the existence of most of the 15 proposed subspecies of T. graeca is weak.

Authors+Show Affiliations

Dept. of Human Retrovirology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. a.c.vanderkuyl@amc.uva.nlNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15836787

Citation

van der Kuyl, Antoinette C., et al. "Mitochondrial Haplotype Diversity in the Tortoise Species Testudo Graeca From North Africa and the Middle East." BMC Evolutionary Biology, vol. 5, 2005, p. 29.
van der Kuyl AC, Ballasina DL, Zorgdrager F. Mitochondrial haplotype diversity in the tortoise species Testudo graeca from North Africa and the Middle East. BMC Evol Biol. 2005;5:29.
van der Kuyl, A. C., Ballasina, D. L., & Zorgdrager, F. (2005). Mitochondrial haplotype diversity in the tortoise species Testudo graeca from North Africa and the Middle East. BMC Evolutionary Biology, 5, p. 29.
van der Kuyl AC, Ballasina DL, Zorgdrager F. Mitochondrial Haplotype Diversity in the Tortoise Species Testudo Graeca From North Africa and the Middle East. BMC Evol Biol. 2005 Apr 18;5:29. PubMed PMID: 15836787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitochondrial haplotype diversity in the tortoise species Testudo graeca from North Africa and the Middle East. AU - van der Kuyl,Antoinette C, AU - Ballasina,Donato L P, AU - Zorgdrager,Fokla, Y1 - 2005/04/18/ PY - 2004/12/21/received PY - 2005/04/18/accepted PY - 2005/4/20/pubmed PY - 2006/4/14/medline PY - 2005/4/20/entrez SP - 29 EP - 29 JF - BMC evolutionary biology JO - BMC Evol. Biol. VL - 5 N2 - BACKGROUND: To help conservation programs of the endangered spur-thighed tortoise and to gain better insight into its systematics, genetic variation and evolution in the tortoise species Testudo graeca (Testudines: Testudinidae) was investigated by sequence analysis of a 394-nucleotide fragment of the mitochondrial 12S rRNA gene for 158 tortoise specimens belonging to the subspecies Testudo graeca graeca, Testudo graeca ibera, Testudo graeca terrestris, and a newly recognized subspecies Testudo graeca whitei. A 411-nucleotide fragment of the mitochondrial D-loop was additionally sequenced for a subset of 22 T. graeca, chosen because of their 12S gene haplotype and/or geographical origin. RESULTS: Haplotype networks generated by maximum-likelihood and neighbor-joining analyses of both the separate and the combined sequence data sets suggested the existence of two main clades of Testudo graeca, comprising Testudo graeca from northern Africa and Testudo graeca from the Turkey and the Middle East, respectively. CONCLUSION: Mitochondrial DNA haplotyping suggests that the tortoise subspecies of T. g. graeca and T. g. ibera are genetically distinct, with a calculated divergence time in the early or middle Pleistocene. Other proposed subspecies could not clearly be recognized based upon their mt haplotypes and phylogenetic position, and were either part of the T. g. graeca or of the T. g. ibera clade, suggesting that genetic evidence for the existence of most of the 15 proposed subspecies of T. graeca is weak. SN - 1471-2148 UR - https://www.unboundmedicine.com/medline/citation/15836787/Mitochondrial_haplotype_diversity_in_the_tortoise_species_Testudo_graeca_from_North_Africa_and_the_Middle_East_ L2 - https://bmcevolbiol.biomedcentral.com/articles/10.1186/1471-2148-5-29 DB - PRIME DP - Unbound Medicine ER -