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Protective effects of Delta(9)-tetrahydrocannabinol against N-methyl-d-aspartate-induced AF5 cell death.
Brain Res Mol Brain Res. 2005 Apr 04; 134(2):215-25.BR

Abstract

The neuroprotective effects of Delta(9)-tetrahydrocannabinol (THC) were examined using an in vitro model in which the AF5 CNS cell line was exposed to toxic levels of N-methyl-d-aspartate (NMDA), an agonist of the NMDA glutamate receptor. NMDA toxicity was reduced by THC, but not by the more specific cannabinoid receptor agonist, WIN55,212-2. Addition of dibutyryl cAMP (dbcAMP) to the culture medium did not alter the neuroprotective effect of THC and did not unmask a neuroprotective effect of WIN55,212-2. The cannabinoid antagonist SR141716A did not inhibit the neuroprotection induced by THC or alter the response to WIN55,212-2, even in the presence of dbcAMP, indicating that the neuroprotective effect of THC was cannabinoid receptor-independent. On the other hand, both THC and WIN55,212-2 produced cellular toxicology at higher dosages, an effect which was blocked in part by SR141716A. Capsaicin, an antioxidant and vanilloid receptor agonist, also produced a protective effect against NMDA toxicology. The protective effect of capsaicin was blocked by co-application of ruthenium red, but was not blocked by the specific vanilloid receptor antagonist capsazepine, and the transient receptor potential vanilloid type 1 (TRPV1) and ANKTM1 transcripts were not detected in AF5 cells. Thus, the neuroprotective effects of THC and capsaicin did not appear to be mediated by TRP ion channel family receptors. The antioxidant alpha-tocopherol prevented neurotoxicity in a dose-dependent manner. Therefore, THC may function as an antioxidant to increase cell survival in NMDA-induced neurotoxicity in the AF5 cell model, while higher dosages produce toxicity mediated by CB1 receptor stimulation.

Authors+Show Affiliations

Cellular Neurobiology Research Branch, Intramural Research Program, NIDA, National Institutes of Health, Baltimore, MD 21224, USA. jchen@intra.nida.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15836919

Citation

Chen, Jia, et al. "Protective Effects of Delta(9)-tetrahydrocannabinol Against N-methyl-d-aspartate-induced AF5 Cell Death." Brain Research. Molecular Brain Research, vol. 134, no. 2, 2005, pp. 215-25.
Chen J, Lee CT, Errico S, et al. Protective effects of Delta(9)-tetrahydrocannabinol against N-methyl-d-aspartate-induced AF5 cell death. Brain Res Mol Brain Res. 2005;134(2):215-25.
Chen, J., Lee, C. T., Errico, S., Deng, X., Cadet, J. L., & Freed, W. J. (2005). Protective effects of Delta(9)-tetrahydrocannabinol against N-methyl-d-aspartate-induced AF5 cell death. Brain Research. Molecular Brain Research, 134(2), 215-25.
Chen J, et al. Protective Effects of Delta(9)-tetrahydrocannabinol Against N-methyl-d-aspartate-induced AF5 Cell Death. Brain Res Mol Brain Res. 2005 Apr 4;134(2):215-25. PubMed PMID: 15836919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of Delta(9)-tetrahydrocannabinol against N-methyl-d-aspartate-induced AF5 cell death. AU - Chen,Jia, AU - Lee,Chun-Ting, AU - Errico,Stacie, AU - Deng,Xiaolin, AU - Cadet,Jean L, AU - Freed,William J, PY - 2004/06/15/received PY - 2004/09/14/revised PY - 2004/10/24/accepted PY - 2005/4/20/pubmed PY - 2005/7/6/medline PY - 2005/4/20/entrez SP - 215 EP - 25 JF - Brain research. Molecular brain research JO - Brain Res Mol Brain Res VL - 134 IS - 2 N2 - The neuroprotective effects of Delta(9)-tetrahydrocannabinol (THC) were examined using an in vitro model in which the AF5 CNS cell line was exposed to toxic levels of N-methyl-d-aspartate (NMDA), an agonist of the NMDA glutamate receptor. NMDA toxicity was reduced by THC, but not by the more specific cannabinoid receptor agonist, WIN55,212-2. Addition of dibutyryl cAMP (dbcAMP) to the culture medium did not alter the neuroprotective effect of THC and did not unmask a neuroprotective effect of WIN55,212-2. The cannabinoid antagonist SR141716A did not inhibit the neuroprotection induced by THC or alter the response to WIN55,212-2, even in the presence of dbcAMP, indicating that the neuroprotective effect of THC was cannabinoid receptor-independent. On the other hand, both THC and WIN55,212-2 produced cellular toxicology at higher dosages, an effect which was blocked in part by SR141716A. Capsaicin, an antioxidant and vanilloid receptor agonist, also produced a protective effect against NMDA toxicology. The protective effect of capsaicin was blocked by co-application of ruthenium red, but was not blocked by the specific vanilloid receptor antagonist capsazepine, and the transient receptor potential vanilloid type 1 (TRPV1) and ANKTM1 transcripts were not detected in AF5 cells. Thus, the neuroprotective effects of THC and capsaicin did not appear to be mediated by TRP ion channel family receptors. The antioxidant alpha-tocopherol prevented neurotoxicity in a dose-dependent manner. Therefore, THC may function as an antioxidant to increase cell survival in NMDA-induced neurotoxicity in the AF5 cell model, while higher dosages produce toxicity mediated by CB1 receptor stimulation. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/15836919/Protective_effects_of_Delta_9__tetrahydrocannabinol_against_N_methyl_d_aspartate_induced_AF5_cell_death_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169-328X(04)00588-1 DB - PRIME DP - Unbound Medicine ER -