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[Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis].
Di Yi Jun Yi Da Xue Xue Bao. 2005 Apr; 25(4):380-3.DY

Abstract

OBJECTIVE

To evaluate the specificity and sensitivity of immunodiagnosis with the antigen with molecular mass of 32 000 of Angiostrongyliasis cantonensis(AC32).

METHODS

The major antigenic protein AC32 was purified from the antigens of A.cantonensis adult worm by electroelution from SDS-polyacrylamide gels. AC32-enzyme linked immunosorbent assay (ELISA) and adult worm antigen (AWA)-ELISA were used to detect the specific IgG in the sera of normal rats (n=5) and rats with angiostrongyliasis (n=61), sera of healthy individuals (n=50) and patients (n=50) with angiostrongyliasis, schistosomiasis and other parasitosis.

RESULTS

The positivity rate in AC32-ELISA of the sera from rats with angiostrongyliasis was 100%; without false positive results or cross reaction between AC32 and the sera of the normal control and patients infected with parasites other than A.cantonensis.

CONCLUSION

AC32 of A.cantonensis is a valuable candidate antigen for immunodiagnosis of angiostrongyliasis with high sensitivity and specificity.

Authors+Show Affiliations

Department of Parasitology, Southern Medical University, Guangzhou 510515, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15837632

Citation

Li, Hua, et al. "[Value of the Antigen With Molecular Mass of 32 000 in Immunodiagnosis of Angiostrongylus Cantonensis]." Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, vol. 25, no. 4, 2005, pp. 380-3.
Li H, Chen XG, Shen HX, et al. [Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis]. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(4):380-3.
Li, H., Chen, X. G., Shen, H. X., Chen, D. X., Qiu, Y. R., & Hu, X. J. (2005). [Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis]. Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, 25(4), 380-3.
Li H, et al. [Value of the Antigen With Molecular Mass of 32 000 in Immunodiagnosis of Angiostrongylus Cantonensis]. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(4):380-3. PubMed PMID: 15837632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis]. AU - Li,Hua, AU - Chen,Xiao-Guang, AU - Shen,Hao-Xian, AU - Chen,Dai-Xiong, AU - Qiu,Yu-Rong, AU - Hu,Xiao-Jia, PY - 2005/4/20/pubmed PY - 2007/5/2/medline PY - 2005/4/20/entrez SP - 380 EP - 3 JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA JO - Di Yi Jun Yi Da Xue Xue Bao VL - 25 IS - 4 N2 - OBJECTIVE: To evaluate the specificity and sensitivity of immunodiagnosis with the antigen with molecular mass of 32 000 of Angiostrongyliasis cantonensis(AC32). METHODS: The major antigenic protein AC32 was purified from the antigens of A.cantonensis adult worm by electroelution from SDS-polyacrylamide gels. AC32-enzyme linked immunosorbent assay (ELISA) and adult worm antigen (AWA)-ELISA were used to detect the specific IgG in the sera of normal rats (n=5) and rats with angiostrongyliasis (n=61), sera of healthy individuals (n=50) and patients (n=50) with angiostrongyliasis, schistosomiasis and other parasitosis. RESULTS: The positivity rate in AC32-ELISA of the sera from rats with angiostrongyliasis was 100%; without false positive results or cross reaction between AC32 and the sera of the normal control and patients infected with parasites other than A.cantonensis. CONCLUSION: AC32 of A.cantonensis is a valuable candidate antigen for immunodiagnosis of angiostrongyliasis with high sensitivity and specificity. SN - 1000-2588 UR - https://www.unboundmedicine.com/medline/citation/15837632/[Value_of_the_antigen_with_molecular_mass_of_32_000_in_immunodiagnosis_of_Angiostrongylus_cantonensis]_ DB - PRIME DP - Unbound Medicine ER -