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Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertions in the gene.
Plant J. 2005 May; 42(3):353-63.PJ

Abstract

Bright blue or red flowers in the Japanese morning glory (Ipomoea nil) contain anthocyanidin 3-O-sophoroside derivatives, whereas the reddish-brown or purplish-gray petals in its dusky mutants accumulate anthocyanidin 3-O-glucoside derivatives. The Dusky gene was found to encode a novel glucosyltransferase, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase (3GGT), which mediates the glucosylation of anthocyanidin 3-O-glucosides to yield anthocyanidin 3-O-sophorosides. Ipomoea nil carries one copy of the 3GGT gene that contains no intron and produces 1.6-kbp transcripts mainly in the petals and tubes of flower buds at around 24 h before flower opening. The gene products of both In3GGT in I. nil and Ip3GGT in the common morning glory (Ipomoea purpurea) comprise 459 amino acids and showed a close relationship to the petunia UDP-rhamnose:anthocyanidin 3-O-glucoside-6''-O-rhamnosyltransferase (3RT), which controls the addition of a rhamnose molecule to anthocyanidin 3-O-glucosides for conversion into anthocyanidin 3-O-rutinosides. All of the 30 dusky mutants tested were found to carry the 4-bp insertion mutations GGAT or CGAT at an identical position near the 3' end of the gene, and the insertions caused frameshift mutations. The expected 3GGT enzymatic activities were found in the crude extracts of Escherichia coli, in which the 3GGT cDNA of I. nil or I. purpurea was expressed, while no such activity was detected in the extracts expressed with the dusky mutant cDNAs containing 4-bp insertions. Moreover, the introduced Ip3GGT cDNA efficiently produced 3GGT that converted cyanidin 3-O-glucoside into cyanidin 3-O-sophoroside in transgenic petunia plants.

Authors+Show Affiliations

National Institute for Basic Biology, Okazaki 444-8585, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15842621

Citation

Morita, Yasumasa, et al. "Japanese Morning Glory Dusky Mutants Displaying Reddish-brown or Purplish-gray Flowers Are Deficient in a Novel Glycosylation Enzyme for Anthocyanin Biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, Due to 4-bp Insertions in the Gene." The Plant Journal : for Cell and Molecular Biology, vol. 42, no. 3, 2005, pp. 353-63.
Morita Y, Hoshino A, Kikuchi Y, et al. Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertions in the gene. Plant J. 2005;42(3):353-63.
Morita, Y., Hoshino, A., Kikuchi, Y., Okuhara, H., Ono, E., Tanaka, Y., Fukui, Y., Saito, N., Nitasaka, E., Noguchi, H., & Iida, S. (2005). Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertions in the gene. The Plant Journal : for Cell and Molecular Biology, 42(3), 353-63.
Morita Y, et al. Japanese Morning Glory Dusky Mutants Displaying Reddish-brown or Purplish-gray Flowers Are Deficient in a Novel Glycosylation Enzyme for Anthocyanin Biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, Due to 4-bp Insertions in the Gene. Plant J. 2005;42(3):353-63. PubMed PMID: 15842621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertions in the gene. AU - Morita,Yasumasa, AU - Hoshino,Atsushi, AU - Kikuchi,Yasumasa, AU - Okuhara,Hiroaki, AU - Ono,Eiichiro, AU - Tanaka,Yoshikazu, AU - Fukui,Yuko, AU - Saito,Norio, AU - Nitasaka,Eiji, AU - Noguchi,Hiroshi, AU - Iida,Shigeru, PY - 2005/4/22/pubmed PY - 2005/7/13/medline PY - 2005/4/22/entrez SP - 353 EP - 63 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 42 IS - 3 N2 - Bright blue or red flowers in the Japanese morning glory (Ipomoea nil) contain anthocyanidin 3-O-sophoroside derivatives, whereas the reddish-brown or purplish-gray petals in its dusky mutants accumulate anthocyanidin 3-O-glucoside derivatives. The Dusky gene was found to encode a novel glucosyltransferase, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase (3GGT), which mediates the glucosylation of anthocyanidin 3-O-glucosides to yield anthocyanidin 3-O-sophorosides. Ipomoea nil carries one copy of the 3GGT gene that contains no intron and produces 1.6-kbp transcripts mainly in the petals and tubes of flower buds at around 24 h before flower opening. The gene products of both In3GGT in I. nil and Ip3GGT in the common morning glory (Ipomoea purpurea) comprise 459 amino acids and showed a close relationship to the petunia UDP-rhamnose:anthocyanidin 3-O-glucoside-6''-O-rhamnosyltransferase (3RT), which controls the addition of a rhamnose molecule to anthocyanidin 3-O-glucosides for conversion into anthocyanidin 3-O-rutinosides. All of the 30 dusky mutants tested were found to carry the 4-bp insertion mutations GGAT or CGAT at an identical position near the 3' end of the gene, and the insertions caused frameshift mutations. The expected 3GGT enzymatic activities were found in the crude extracts of Escherichia coli, in which the 3GGT cDNA of I. nil or I. purpurea was expressed, while no such activity was detected in the extracts expressed with the dusky mutant cDNAs containing 4-bp insertions. Moreover, the introduced Ip3GGT cDNA efficiently produced 3GGT that converted cyanidin 3-O-glucoside into cyanidin 3-O-sophoroside in transgenic petunia plants. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/15842621/Japanese_morning_glory_dusky_mutants_displaying_reddish_brown_or_purplish_gray_flowers_are_deficient_in_a_novel_glycosylation_enzyme_for_anthocyanin_biosynthesis_UDP_glucose:anthocyanidin_3_O_glucoside_2''_O_glucosyltransferase_due_to_4_bp_insertions_in_the_gene_ L2 - https://doi.org/10.1111/j.1365-313X.2005.02383.x DB - PRIME DP - Unbound Medicine ER -