Tags

Type your tag names separated by a space and hit enter

Evaluation of the Cedia heroin metabolite (6-AM) immunoassay with urine specimens from A criminal justice drug-testing program.
J Anal Toxicol. 2005 Apr; 29(3):201-4.JA

Abstract

The ability to differentiate illicit from legitimate drug use in a drug-testing program would decrease costs by reducing the number of screening specimens requiring confirmation and also reduce the stigma attached to positive preliminary test results. Because many screening tests for drug detection use immunoassays, increasing the specificity of these tests has been a goal of manufacturers. In this study we evaluated the utility of one such assay, the Cedia heroin metabolite (6-acetylmorphine, 6-AM) assay to reliably detect heroin use. Specimens (N = 525) from a criminal justice drug-testing program were screened with this assay (cutoff concentration = 10 ng/mL 6-AM) and any positive samples were confirmed by gas chromatographic-mass spectrometric analysis (lower reporting limit for 6-AM = 5 ng/mL). The confirmation rate for the enzyme immunoassay (EIA) was 98% (517/525). Specimens contained 6-AM at concentrations ranging from 5 to 16,923 ng/mL (mean = 1251; median = 317). All confirmed specimens also contained morphine (range: 8-222,427 ng/mL; mean = 11,203 ; median = 4134). When challenged with standard drug solutions, the EIA correctly identified drug-free urine and produced positive results (lowest concentration, in ng/mL, that produced a positive result) with morphine at 10,000; oxycodone at 61,000; codeine at 60,000; hydromorphone at 10,000; hydrocodone at 60,000, 6-AM at 10, and pentazocine at 35,000 ng/mL. The Cedia heroin metabolite (6-AM) assay produced a high confirmation rate when challenged with urine specimens and therefore should be a useful tool in forensic toxicology. Potential users should be aware that high concentrations of other opioids (e.g., morphine, oxycodone) and structurally related compounds (e.g., pentazocine) may produce positive results.

Authors+Show Affiliations

The Office of the Cuyahoga County Coroner, 11001 Cedar Ave., Cleveland, OH 44106, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15842764

Citation

Jenkins, Amanda J., et al. "Evaluation of the Cedia Heroin Metabolite (6-AM) Immunoassay With Urine Specimens From a Criminal Justice Drug-testing Program." Journal of Analytical Toxicology, vol. 29, no. 3, 2005, pp. 201-4.
Jenkins AJ, Lavins ES, Snyder A. Evaluation of the Cedia heroin metabolite (6-AM) immunoassay with urine specimens from A criminal justice drug-testing program. J Anal Toxicol. 2005;29(3):201-4.
Jenkins, A. J., Lavins, E. S., & Snyder, A. (2005). Evaluation of the Cedia heroin metabolite (6-AM) immunoassay with urine specimens from A criminal justice drug-testing program. Journal of Analytical Toxicology, 29(3), 201-4.
Jenkins AJ, Lavins ES, Snyder A. Evaluation of the Cedia Heroin Metabolite (6-AM) Immunoassay With Urine Specimens From a Criminal Justice Drug-testing Program. J Anal Toxicol. 2005;29(3):201-4. PubMed PMID: 15842764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the Cedia heroin metabolite (6-AM) immunoassay with urine specimens from A criminal justice drug-testing program. AU - Jenkins,Amanda J, AU - Lavins,Eric S, AU - Snyder,Ann, PY - 2005/4/22/pubmed PY - 2005/7/12/medline PY - 2005/4/22/entrez SP - 201 EP - 4 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 29 IS - 3 N2 - The ability to differentiate illicit from legitimate drug use in a drug-testing program would decrease costs by reducing the number of screening specimens requiring confirmation and also reduce the stigma attached to positive preliminary test results. Because many screening tests for drug detection use immunoassays, increasing the specificity of these tests has been a goal of manufacturers. In this study we evaluated the utility of one such assay, the Cedia heroin metabolite (6-acetylmorphine, 6-AM) assay to reliably detect heroin use. Specimens (N = 525) from a criminal justice drug-testing program were screened with this assay (cutoff concentration = 10 ng/mL 6-AM) and any positive samples were confirmed by gas chromatographic-mass spectrometric analysis (lower reporting limit for 6-AM = 5 ng/mL). The confirmation rate for the enzyme immunoassay (EIA) was 98% (517/525). Specimens contained 6-AM at concentrations ranging from 5 to 16,923 ng/mL (mean = 1251; median = 317). All confirmed specimens also contained morphine (range: 8-222,427 ng/mL; mean = 11,203 ; median = 4134). When challenged with standard drug solutions, the EIA correctly identified drug-free urine and produced positive results (lowest concentration, in ng/mL, that produced a positive result) with morphine at 10,000; oxycodone at 61,000; codeine at 60,000; hydromorphone at 10,000; hydrocodone at 60,000, 6-AM at 10, and pentazocine at 35,000 ng/mL. The Cedia heroin metabolite (6-AM) assay produced a high confirmation rate when challenged with urine specimens and therefore should be a useful tool in forensic toxicology. Potential users should be aware that high concentrations of other opioids (e.g., morphine, oxycodone) and structurally related compounds (e.g., pentazocine) may produce positive results. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/15842764/Evaluation_of_the_Cedia_heroin_metabolite__6_AM__immunoassay_with_urine_specimens_from_A_criminal_justice_drug_testing_program_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/29.3.201 DB - PRIME DP - Unbound Medicine ER -