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Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants.
Planta. 2005 Sep; 222(1):192-200.P

Abstract

Peroxisomes, being one of the main organelles where reactive oxygen species (ROS) are both generated and detoxified, have been suggested to be instrumental in redox-mediated plant cell defence against oxidative stress. We studied the involvement of tomato (Lycopersicon esculentum Mill.) leaf peroxisomes in defence response to oxidative stress generated upon Botrytis cinerea Pers. infection. The peroxisomal antioxidant potential expressed as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) and glutathione peroxidase (GSH-Px, EC 1.11.1.19) as well as the ascorbate-glutathione (AA-GSH) cycle activities was monitored. The initial infection-induced increase in SOD, CAT and GSH-Px indicating antioxidant defence activation was followed by a progressive inhibition concomitant with disease symptom development. Likewise, the activities of AA-GSH cycle enzymes: ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1) and glutathione reductase (GR, EC 1.6.4.2) as well as ascorbate and glutathione concentrations and redox ratios were significantly decreased. However, the rate and timing of these events differed. Our results indicate that B. cinerea triggers significant changes in the peroxisomal antioxidant system leading to a collapse of the protective mechanism at advanced stage of infection. These changes appear to be partly the effect of pathogen-promoted leaf senescence.

Authors+Show Affiliations

Department of Plant Physiology and Biochemistry, University of Łódź, 90-237 Łódź, Banacha 12/16, Poland. elkuz@biol.uni.lodz.plNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15843961

Citation

Kuzniak, Elźbieta, and Maria Skłodowska. "Fungal Pathogen-induced Changes in the Antioxidant Systems of Leaf Peroxisomes From Infected Tomato Plants." Planta, vol. 222, no. 1, 2005, pp. 192-200.
Kuzniak E, Skłodowska M. Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta. 2005;222(1):192-200.
Kuzniak, E., & Skłodowska, M. (2005). Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta, 222(1), 192-200.
Kuzniak E, Skłodowska M. Fungal Pathogen-induced Changes in the Antioxidant Systems of Leaf Peroxisomes From Infected Tomato Plants. Planta. 2005;222(1):192-200. PubMed PMID: 15843961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. AU - Kuzniak,Elźbieta, AU - Skłodowska,Maria, Y1 - 2005/04/21/ PY - 2004/10/18/received PY - 2005/02/03/accepted PY - 2005/4/22/pubmed PY - 2006/1/24/medline PY - 2005/4/22/entrez SP - 192 EP - 200 JF - Planta JO - Planta VL - 222 IS - 1 N2 - Peroxisomes, being one of the main organelles where reactive oxygen species (ROS) are both generated and detoxified, have been suggested to be instrumental in redox-mediated plant cell defence against oxidative stress. We studied the involvement of tomato (Lycopersicon esculentum Mill.) leaf peroxisomes in defence response to oxidative stress generated upon Botrytis cinerea Pers. infection. The peroxisomal antioxidant potential expressed as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) and glutathione peroxidase (GSH-Px, EC 1.11.1.19) as well as the ascorbate-glutathione (AA-GSH) cycle activities was monitored. The initial infection-induced increase in SOD, CAT and GSH-Px indicating antioxidant defence activation was followed by a progressive inhibition concomitant with disease symptom development. Likewise, the activities of AA-GSH cycle enzymes: ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1) and glutathione reductase (GR, EC 1.6.4.2) as well as ascorbate and glutathione concentrations and redox ratios were significantly decreased. However, the rate and timing of these events differed. Our results indicate that B. cinerea triggers significant changes in the peroxisomal antioxidant system leading to a collapse of the protective mechanism at advanced stage of infection. These changes appear to be partly the effect of pathogen-promoted leaf senescence. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/15843961/Fungal_pathogen_induced_changes_in_the_antioxidant_systems_of_leaf_peroxisomes_from_infected_tomato_plants_ L2 - https://dx.doi.org/10.1007/s00425-005-1514-8 DB - PRIME DP - Unbound Medicine ER -