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Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons.
J Neurosci Res. 2005 May 15; 80(4):491-500.JN

Abstract

In rat hippocampal neurons cultured with the antagonist for N-methyl-D-aspartate (NMDA) receptors dizocilpine (MK-801) for 8 days in vitro (DIV), a significant decrease was seen in the expression of microtubule-associated protein-2 (MAP-2) as well as mRNA for both brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43), in addition to decreased viability. MK-801 not only decreased the expression of the NR1 subunit of NMDA receptors but also increased NR2A expression, without affecting NR2B expression. Repetitive daily exposure to static magnetic fields at 100 mT for 15 min led to a decrease in the expression of MAP-2, without significantly affecting cell viability or the expression of neuronal nuclei (NeuN) and GAP-43. However, the repetitive magnetism prevented decreases in both BDNF mRNA and MAP-2 and additionally increased the expression of NR2A subunit, without altering NR1 expression in neurons cultured in the presence of MK-801. Repetitive magnetism was also effective in preventing the decrease by MK-801 in the ability of NMDA to increase intracellular free Ca2+ ions, without affecting the decrease in the maximal response. These results suggest that repetitive magnetism may at least in part counteract the neurotoxicity of MK-801 through modulation of the expression of particular NMDA receptor subunits in cultured rat hippocampal neurons.

Authors+Show Affiliations

Hirai T
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kakuma-machi, Kanazawa, Ishikawa, Japan.
Taniura H
No affiliation info available
Goto Y
No affiliation info available
Tamaki K
No affiliation info available
Oikawa H
No affiliation info available
Kambe Y
No affiliation info available
Ogura M
No affiliation info available
Ohno Y
No affiliation info available
Takarada T
No affiliation info available
Yoneda Y
No affiliation info available

MeSH

Analysis of VarianceAnimalsBlotting, WesternBrain-Derived Neurotrophic FactorCalciumCell SurvivalCells, CulturedDizocilpine MaleateDose-Response Relationship, DrugEmbryo, MammalianExcitatory Amino Acid AntagonistsExtracellular SpaceGAP-43 ProteinGene Expression RegulationHippocampusMagneticsMicrotubule-Associated ProteinsN-MethylaspartateNeuronsPhosphopyruvate HydrataseRNA, MessengerRatsRats, WistarReceptors, N-Methyl-D-AspartateReverse Transcriptase Polymerase Chain ReactionTime Factors

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15846781

Citation

Hirai, Takao, et al. "Counteraction By Repetitive Daily Exposure to Static Magnetism Against Sustained Blockade of N-methyl-D-aspartate Receptor Channels in Cultured Rat Hippocampal Neurons." Journal of Neuroscience Research, vol. 80, no. 4, 2005, pp. 491-500.
Hirai T, Taniura H, Goto Y, et al. Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons. J Neurosci Res. 2005;80(4):491-500.
Hirai, T., Taniura, H., Goto, Y., Tamaki, K., Oikawa, H., Kambe, Y., Ogura, M., Ohno, Y., Takarada, T., & Yoneda, Y. (2005). Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons. Journal of Neuroscience Research, 80(4), 491-500.
Hirai T, et al. Counteraction By Repetitive Daily Exposure to Static Magnetism Against Sustained Blockade of N-methyl-D-aspartate Receptor Channels in Cultured Rat Hippocampal Neurons. J Neurosci Res. 2005 May 15;80(4):491-500. PubMed PMID: 15846781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons. AU - Hirai,Takao, AU - Taniura,Hideo, AU - Goto,Yasuaki, AU - Tamaki,Keisuke, AU - Oikawa,Hirotaka, AU - Kambe,Yuki, AU - Ogura,Masato, AU - Ohno,Yu, AU - Takarada,Takeshi, AU - Yoneda,Yukio, PY - 2005/4/23/pubmed PY - 2005/7/26/medline PY - 2005/4/23/entrez SP - 491 EP - 500 JF - Journal of neuroscience research JO - J Neurosci Res VL - 80 IS - 4 N2 - In rat hippocampal neurons cultured with the antagonist for N-methyl-D-aspartate (NMDA) receptors dizocilpine (MK-801) for 8 days in vitro (DIV), a significant decrease was seen in the expression of microtubule-associated protein-2 (MAP-2) as well as mRNA for both brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43), in addition to decreased viability. MK-801 not only decreased the expression of the NR1 subunit of NMDA receptors but also increased NR2A expression, without affecting NR2B expression. Repetitive daily exposure to static magnetic fields at 100 mT for 15 min led to a decrease in the expression of MAP-2, without significantly affecting cell viability or the expression of neuronal nuclei (NeuN) and GAP-43. However, the repetitive magnetism prevented decreases in both BDNF mRNA and MAP-2 and additionally increased the expression of NR2A subunit, without altering NR1 expression in neurons cultured in the presence of MK-801. Repetitive magnetism was also effective in preventing the decrease by MK-801 in the ability of NMDA to increase intracellular free Ca2+ ions, without affecting the decrease in the maximal response. These results suggest that repetitive magnetism may at least in part counteract the neurotoxicity of MK-801 through modulation of the expression of particular NMDA receptor subunits in cultured rat hippocampal neurons. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/15846781/Counteraction_by_repetitive_daily_exposure_to_static_magnetism_against_sustained_blockade_of_N_methyl_D_aspartate_receptor_channels_in_cultured_rat_hippocampal_neurons_ DB - PRIME DP - Unbound Medicine ER -