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[Generation of attenuated H5N1 and H5N2 subtypes of influenza virus recombinants by reverse genetics system].
Wei Sheng Wu Xue Bao. 2005 Feb; 45(1):53-7.WS

Abstract

The HA connecting peptide at cleavage site, PQRERRKKR / GL, of an H5N1 subtype avian influenza virus (AIV) was replaced with PQRESR / GL, and then the modified HA gene was cloned into the transcription/expression vector, pHW2000, constructing a plasmid named pHW524-HA. The NA (N1) gene from the H5N1 virus and the NA (N2) gene from an H9N2 AIV were also cloned into pHW2000 separately, resulting in plasmids pHW506-NA and pHW206-NA. With the organization of pHW524-HA, pHW506-NA or pHW206-NA, and six plasmids containing internal genes from A/WSN/33 backbone virus, two transfectants, H5N1/WSN and H5N2/WSN, were subsequently generated by eight-plasmid system. After 15 consecutive passages in embryonated eggs, the two recombinants grew up to high titers of 1:2(9) in hemagglutination test with no changes in nucleotide sequences of the surface genes detected. Both the recombinant viruses belonged to mildly pathogen when evaluated by the pathogenicity test in six-week-old SPF chickens. H5N2/WSN recombinant virus was obviously less pathogenic than H5N1/WSN virus for embryonated chicken eggs. This presentation showed that the reverse genetics system is a very useful tool for studying the construction and function of individual genes and for the generation of virus as vaccine candidate.

Authors+Show Affiliations

Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. jianhl@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15847163

Citation

Lu, Jian-hong, et al. "[Generation of Attenuated H5N1 and H5N2 Subtypes of Influenza Virus Recombinants By Reverse Genetics System]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 45, no. 1, 2005, pp. 53-7.
Lu JH, Long JX, Shao WX, et al. [Generation of attenuated H5N1 and H5N2 subtypes of influenza virus recombinants by reverse genetics system]. Wei Sheng Wu Xue Bao. 2005;45(1):53-7.
Lu, J. H., Long, J. X., Shao, W. X., Wei, D. P., & Liu, X. F. (2005). [Generation of attenuated H5N1 and H5N2 subtypes of influenza virus recombinants by reverse genetics system]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 45(1), 53-7.
Lu JH, et al. [Generation of Attenuated H5N1 and H5N2 Subtypes of Influenza Virus Recombinants By Reverse Genetics System]. Wei Sheng Wu Xue Bao. 2005;45(1):53-7. PubMed PMID: 15847163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Generation of attenuated H5N1 and H5N2 subtypes of influenza virus recombinants by reverse genetics system]. AU - Lu,Jian-hong, AU - Long,Jin-xue, AU - Shao,Wei-xing, AU - Wei,Dong-ping, AU - Liu,Xiu-fan, PY - 2005/4/26/pubmed PY - 2006/9/2/medline PY - 2005/4/26/entrez SP - 53 EP - 7 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 45 IS - 1 N2 - The HA connecting peptide at cleavage site, PQRERRKKR / GL, of an H5N1 subtype avian influenza virus (AIV) was replaced with PQRESR / GL, and then the modified HA gene was cloned into the transcription/expression vector, pHW2000, constructing a plasmid named pHW524-HA. The NA (N1) gene from the H5N1 virus and the NA (N2) gene from an H9N2 AIV were also cloned into pHW2000 separately, resulting in plasmids pHW506-NA and pHW206-NA. With the organization of pHW524-HA, pHW506-NA or pHW206-NA, and six plasmids containing internal genes from A/WSN/33 backbone virus, two transfectants, H5N1/WSN and H5N2/WSN, were subsequently generated by eight-plasmid system. After 15 consecutive passages in embryonated eggs, the two recombinants grew up to high titers of 1:2(9) in hemagglutination test with no changes in nucleotide sequences of the surface genes detected. Both the recombinant viruses belonged to mildly pathogen when evaluated by the pathogenicity test in six-week-old SPF chickens. H5N2/WSN recombinant virus was obviously less pathogenic than H5N1/WSN virus for embryonated chicken eggs. This presentation showed that the reverse genetics system is a very useful tool for studying the construction and function of individual genes and for the generation of virus as vaccine candidate. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/15847163/[Generation_of_attenuated_H5N1_and_H5N2_subtypes_of_influenza_virus_recombinants_by_reverse_genetics_system]_ L2 - https://medlineplus.gov/birdflu.html DB - PRIME DP - Unbound Medicine ER -