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Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses.
J Virol Methods. 2005 Jun; 126(1-2):119-25.JV

Abstract

A diagnostic method to distinguish between West Nile virus (WNV) and Japanese encephalitis virus (JEV) based on fluorogenic real-time polymerase chain reaction (TaqMan) assays was developed. To detect WNV and JEV with a single probe, a probe was designed to correspond to sequences in the core protein region that are shared by both viruses. The specificity of this assay depended on the primer sets used, which were specific to the target virus sequences: the primer set for WNV could detect only WNV strains and the primer set for JEV could detect only JEV strains. The assays were tested by detection of viruses from experimentally infected animal tissues. The method described in this study will be useful for the simultaneous discrimination of WNV and JEV in areas where JEV is endemic, such as East Asia.

Authors+Show Affiliations

Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Sapporo, Hokkaido 060-0818, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15847927

Citation

Shirato, Kazuya, et al. "Detection of West Nile Virus and Japanese Encephalitis Virus Using Real-time PCR With a Probe Common to Both Viruses." Journal of Virological Methods, vol. 126, no. 1-2, 2005, pp. 119-25.
Shirato K, Miyoshi H, Kariwa H, et al. Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses. J Virol Methods. 2005;126(1-2):119-25.
Shirato, K., Miyoshi, H., Kariwa, H., & Takashima, I. (2005). Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses. Journal of Virological Methods, 126(1-2), 119-25.
Shirato K, et al. Detection of West Nile Virus and Japanese Encephalitis Virus Using Real-time PCR With a Probe Common to Both Viruses. J Virol Methods. 2005;126(1-2):119-25. PubMed PMID: 15847927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses. AU - Shirato,Kazuya, AU - Miyoshi,Hirotsugu, AU - Kariwa,Hiroaki, AU - Takashima,Ikuo, PY - 2004/11/02/received PY - 2005/02/02/revised PY - 2005/02/03/accepted PY - 2005/4/26/pubmed PY - 2005/9/24/medline PY - 2005/4/26/entrez SP - 119 EP - 25 JF - Journal of virological methods JO - J Virol Methods VL - 126 IS - 1-2 N2 - A diagnostic method to distinguish between West Nile virus (WNV) and Japanese encephalitis virus (JEV) based on fluorogenic real-time polymerase chain reaction (TaqMan) assays was developed. To detect WNV and JEV with a single probe, a probe was designed to correspond to sequences in the core protein region that are shared by both viruses. The specificity of this assay depended on the primer sets used, which were specific to the target virus sequences: the primer set for WNV could detect only WNV strains and the primer set for JEV could detect only JEV strains. The assays were tested by detection of viruses from experimentally infected animal tissues. The method described in this study will be useful for the simultaneous discrimination of WNV and JEV in areas where JEV is endemic, such as East Asia. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/15847927/Detection_of_West_Nile_virus_and_Japanese_encephalitis_virus_using_real_time_PCR_with_a_probe_common_to_both_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(05)00045-5 DB - PRIME DP - Unbound Medicine ER -