Tags

Type your tag names separated by a space and hit enter

Design of a phytosphingosine-containing, positively-charged nanoemulsion as a colloidal carrier system for dermal application of ceramides.
Eur J Pharm Biopharm. 2005 May; 60(1):91-8.EJ

Abstract

Positively charged oil/water (o/w) nanoemulsions (PN) are effective vehicles to change the permeability of the skin. This study focused on the preparation and characterisation of phytosphingosine (PS) containing PN (PPN) which serve as colloidal carriers for the dermal application of ceramide IIIB (CIIIB) and the stratum corneum (SC) lipids (PPNSC) such as ceramide III (CIII), cholesterol, and palmitic acid. The investigations were conducted using appropriate emulsification and homogenisation processing conditions to optimise PPNSC with regard to droplet size, physical stability, and solubility of PS, CIII and CIIIB. A decrease in droplet size was observed through eight homogenisation cycles at a pressure of 500 bar and a temperature of 50 degrees C. Above these optimal values, an increase in droplet size was observed. PS and ceramides have low solubilities in oil and water. When Lipoid E-80 (LE80) was added to the oil phase, the solubility of PS and ceramides increased, indicating some interactions shown by DSC measurements. SC lipids and CIIIB could be successfully incorporated in PPN without producing any physical instability. The high stability of PPNSC is probably due to the presence of a hydrophilic (Tween 80) and a lipophilic surfactant (LE80), supported by the lipophilic cosurfactant PS, at the o/w interface. It was shown that PS was responsible for the positive charge and thus supported the high physical stability of PPNSC. This optimised emulsion was selected for further skin absorption evaluation.

Authors+Show Affiliations

Institute of Pharmacy, Free University of Berlin, Berlin, Germany.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15848061

Citation

Yilmaz, Erol, and Hans-Hubert Borchert. "Design of a Phytosphingosine-containing, Positively-charged Nanoemulsion as a Colloidal Carrier System for Dermal Application of Ceramides." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 60, no. 1, 2005, pp. 91-8.
Yilmaz E, Borchert HH. Design of a phytosphingosine-containing, positively-charged nanoemulsion as a colloidal carrier system for dermal application of ceramides. Eur J Pharm Biopharm. 2005;60(1):91-8.
Yilmaz, E., & Borchert, H. H. (2005). Design of a phytosphingosine-containing, positively-charged nanoemulsion as a colloidal carrier system for dermal application of ceramides. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 60(1), 91-8.
Yilmaz E, Borchert HH. Design of a Phytosphingosine-containing, Positively-charged Nanoemulsion as a Colloidal Carrier System for Dermal Application of Ceramides. Eur J Pharm Biopharm. 2005;60(1):91-8. PubMed PMID: 15848061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Design of a phytosphingosine-containing, positively-charged nanoemulsion as a colloidal carrier system for dermal application of ceramides. AU - Yilmaz,Erol, AU - Borchert,Hans-Hubert, PY - 2004/09/24/received PY - 2004/11/01/revised PY - 2004/11/01/accepted PY - 2005/4/26/pubmed PY - 2005/9/24/medline PY - 2005/4/26/entrez SP - 91 EP - 8 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 60 IS - 1 N2 - Positively charged oil/water (o/w) nanoemulsions (PN) are effective vehicles to change the permeability of the skin. This study focused on the preparation and characterisation of phytosphingosine (PS) containing PN (PPN) which serve as colloidal carriers for the dermal application of ceramide IIIB (CIIIB) and the stratum corneum (SC) lipids (PPNSC) such as ceramide III (CIII), cholesterol, and palmitic acid. The investigations were conducted using appropriate emulsification and homogenisation processing conditions to optimise PPNSC with regard to droplet size, physical stability, and solubility of PS, CIII and CIIIB. A decrease in droplet size was observed through eight homogenisation cycles at a pressure of 500 bar and a temperature of 50 degrees C. Above these optimal values, an increase in droplet size was observed. PS and ceramides have low solubilities in oil and water. When Lipoid E-80 (LE80) was added to the oil phase, the solubility of PS and ceramides increased, indicating some interactions shown by DSC measurements. SC lipids and CIIIB could be successfully incorporated in PPN without producing any physical instability. The high stability of PPNSC is probably due to the presence of a hydrophilic (Tween 80) and a lipophilic surfactant (LE80), supported by the lipophilic cosurfactant PS, at the o/w interface. It was shown that PS was responsible for the positive charge and thus supported the high physical stability of PPNSC. This optimised emulsion was selected for further skin absorption evaluation. SN - 0939-6411 UR - https://www.unboundmedicine.com/medline/citation/15848061/Design_of_a_phytosphingosine_containing_positively_charged_nanoemulsion_as_a_colloidal_carrier_system_for_dermal_application_of_ceramides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-6411(04)00328-5 DB - PRIME DP - Unbound Medicine ER -