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Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry.
Biosci Biotechnol Biochem. 2005 Apr; 69(4):778-83.BB

Abstract

A method for quantifying indole-3-acetic acid (IAA) and its conjugates with the six amino acids, Ala, -Asp, -Ile, -Glu, -Phe and -Val, in rice (Oryza sativa) by using high-performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (HPLC-ESI-MS/MS) is described. Samples from the rice plant or callus were treated with 80% acetone in water containing 2.5 mM diethyl dithiocarbamate. Each extract was partially purified in C18 cartridge column for solid-phase extraction (SPE) and subjected to HPLC-ESI-MS/MS without converting the product. The detection limit was 3.8 fmol for IAA, and 0.4-2.9 fmol for the IAA amino acid conjugates. The method was applied to the analysis of IAA and its conjugates in rice seedlings, dehulled rice and calli, using 20-100 mg tissue samples.

Authors+Show Affiliations

Plant Functions and Their Control, CREST, Japan Science and Technology Agency, Tokyo 103-0027, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15849417

Citation

Matsuda, Fumio, et al. "Quantification of Indole-3-acetic Acid and Amino Acid Conjugates in Rice By Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry." Bioscience, Biotechnology, and Biochemistry, vol. 69, no. 4, 2005, pp. 778-83.
Matsuda F, Miyazawa H, Wakasa K, et al. Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry. Biosci Biotechnol Biochem. 2005;69(4):778-83.
Matsuda, F., Miyazawa, H., Wakasa, K., & Miyagawa, H. (2005). Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry. Bioscience, Biotechnology, and Biochemistry, 69(4), 778-83.
Matsuda F, et al. Quantification of Indole-3-acetic Acid and Amino Acid Conjugates in Rice By Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry. Biosci Biotechnol Biochem. 2005;69(4):778-83. PubMed PMID: 15849417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry. AU - Matsuda,Fumio, AU - Miyazawa,Haruna, AU - Wakasa,Kyo, AU - Miyagawa,Hisashi, PY - 2005/4/26/pubmed PY - 2005/9/13/medline PY - 2005/4/26/entrez SP - 778 EP - 83 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 69 IS - 4 N2 - A method for quantifying indole-3-acetic acid (IAA) and its conjugates with the six amino acids, Ala, -Asp, -Ile, -Glu, -Phe and -Val, in rice (Oryza sativa) by using high-performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (HPLC-ESI-MS/MS) is described. Samples from the rice plant or callus were treated with 80% acetone in water containing 2.5 mM diethyl dithiocarbamate. Each extract was partially purified in C18 cartridge column for solid-phase extraction (SPE) and subjected to HPLC-ESI-MS/MS without converting the product. The detection limit was 3.8 fmol for IAA, and 0.4-2.9 fmol for the IAA amino acid conjugates. The method was applied to the analysis of IAA and its conjugates in rice seedlings, dehulled rice and calli, using 20-100 mg tissue samples. SN - 0916-8451 UR - https://www.unboundmedicine.com/medline/citation/15849417/Quantification_of_indole_3_acetic_acid_and_amino_acid_conjugates_in_rice_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/bbb/69.778?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -