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Identification of the promoter region required for human adiponectin gene transcription: Association with CCAAT/enhancer binding protein-beta and tumor necrosis factor-alpha.
Biochem Biophys Res Commun. 2005 Jun 03; 331(2):484-90.BB

Abstract

Adiponectin, an adipose tissue-specific plasma protein, is involved in insulin sensitizing and has anti-atherosclerotic properties. Plasma levels of adiponectin are decreased in obese individuals and patients with type 2 diabetes with insulin resistance. Tumor necrosis factor-alpha (TNF-alpha) decreases the expression of adiponectin in adipocytes. The aims of the present study were: (1) to identify the promoter region responsible for basal transcription of the human adiponectin gene, and (2) to investigate the mechanism by which adiponectin was regulated by TNF-alpha. The human adiponectin promoter (2.1kb) was isolated and used for luciferase reporter analysis by transient transfection into 3T3-L1 adipocytes. Deletion analysis demonstrated that the promoter region from -676 to +41 was sufficient for basal transcriptional activity. Mutation analysis of putative response elements for sterol regulatory element binding protein (SREBP) (-431 to -423) and CCAAT/enhancer binding protein (C/EBP) (-230 to -224) showed that both elements were required for basal promoter activity. Adiponectin transcription was increased 3-fold in cells that over-expressed constitutively active C/EBP-beta. Electrophoretic mobility shift assay, using nuclear extract from 3T3-L1 cells and the -258 to -199 region as a probe, demonstrated specific DNA-protein binding, which was abolished by TNF-alpha treatment. The present data indicate that the putative response elements for SREBP and C/EBP are required for human adiponectin promoter activity, and that suppression by TNF-alpha may, at least in part, be associated with inactivation of C/EBP-beta.

Authors+Show Affiliations

Unit of Metabolism/Diabetes and Clinical Nutrition, Nagasaki University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15850785

Citation

Kita, Atsushi, et al. "Identification of the Promoter Region Required for Human Adiponectin Gene Transcription: Association With CCAAT/enhancer Binding Protein-beta and Tumor Necrosis Factor-alpha." Biochemical and Biophysical Research Communications, vol. 331, no. 2, 2005, pp. 484-90.
Kita A, Yamasaki H, Kuwahara H, et al. Identification of the promoter region required for human adiponectin gene transcription: Association with CCAAT/enhancer binding protein-beta and tumor necrosis factor-alpha. Biochem Biophys Res Commun. 2005;331(2):484-90.
Kita, A., Yamasaki, H., Kuwahara, H., Moriuchi, A., Fukushima, K., Kobayashi, M., Fukushima, T., Takahashi, R., Abiru, N., Uotani, S., Kawasaki, E., & Eguchi, K. (2005). Identification of the promoter region required for human adiponectin gene transcription: Association with CCAAT/enhancer binding protein-beta and tumor necrosis factor-alpha. Biochemical and Biophysical Research Communications, 331(2), 484-90.
Kita A, et al. Identification of the Promoter Region Required for Human Adiponectin Gene Transcription: Association With CCAAT/enhancer Binding Protein-beta and Tumor Necrosis Factor-alpha. Biochem Biophys Res Commun. 2005 Jun 3;331(2):484-90. PubMed PMID: 15850785.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the promoter region required for human adiponectin gene transcription: Association with CCAAT/enhancer binding protein-beta and tumor necrosis factor-alpha. AU - Kita,Atsushi, AU - Yamasaki,Hironori, AU - Kuwahara,Hironaga, AU - Moriuchi,Akie, AU - Fukushima,Keiko, AU - Kobayashi,Masakazu, AU - Fukushima,Tetsuya, AU - Takahashi,Ryoko, AU - Abiru,Norio, AU - Uotani,Shigeo, AU - Kawasaki,Eiji, AU - Eguchi,Katsumi, PY - 2005/03/01/received PY - 2005/4/27/pubmed PY - 2005/7/22/medline PY - 2005/4/27/entrez SP - 484 EP - 90 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 331 IS - 2 N2 - Adiponectin, an adipose tissue-specific plasma protein, is involved in insulin sensitizing and has anti-atherosclerotic properties. Plasma levels of adiponectin are decreased in obese individuals and patients with type 2 diabetes with insulin resistance. Tumor necrosis factor-alpha (TNF-alpha) decreases the expression of adiponectin in adipocytes. The aims of the present study were: (1) to identify the promoter region responsible for basal transcription of the human adiponectin gene, and (2) to investigate the mechanism by which adiponectin was regulated by TNF-alpha. The human adiponectin promoter (2.1kb) was isolated and used for luciferase reporter analysis by transient transfection into 3T3-L1 adipocytes. Deletion analysis demonstrated that the promoter region from -676 to +41 was sufficient for basal transcriptional activity. Mutation analysis of putative response elements for sterol regulatory element binding protein (SREBP) (-431 to -423) and CCAAT/enhancer binding protein (C/EBP) (-230 to -224) showed that both elements were required for basal promoter activity. Adiponectin transcription was increased 3-fold in cells that over-expressed constitutively active C/EBP-beta. Electrophoretic mobility shift assay, using nuclear extract from 3T3-L1 cells and the -258 to -199 region as a probe, demonstrated specific DNA-protein binding, which was abolished by TNF-alpha treatment. The present data indicate that the putative response elements for SREBP and C/EBP are required for human adiponectin promoter activity, and that suppression by TNF-alpha may, at least in part, be associated with inactivation of C/EBP-beta. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/15850785/Identification_of_the_promoter_region_required_for_human_adiponectin_gene_transcription:_Association_with_CCAAT/enhancer_binding_protein_beta_and_tumor_necrosis_factor_alpha_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(05)00683-2 DB - PRIME DP - Unbound Medicine ER -