Tags

Type your tag names separated by a space and hit enter

Bat2p is essential in Saccharomyces cerevisiae for fusel alcohol production on the non-fermentable carbon source ethanol.
FEMS Yeast Res. 2005 May; 5(8):757-66.FY

Abstract

Branched-chain amino acids (BCAAs) are key substrates in the formation of fusel alcohols, important flavour components in fermented foods. The first step in the catabolic BCAA degradation is a transaminase step, catalyzed by a branched-chain amino acid transaminase (BCAAT). Saccharomyces cerevisiae possesses a mitochondrial and a cytosolic BCAAT, Bat1p and Bat2p, respectively. In order to study the impact of the BCAATs on fusel alcohol production derived from the BCAA metabolism, S. cerevisiae BCAAT-deletion mutants were constructed. The BCAA l-leucine was exogenously supplied during cultivations with mutants of S. cerevisiae. BAT1 deletion is not essential for fusel alcohol production, neither under glucose nor under ethanol growth conditions. The 3-methyl-1-butanol production rate of bat1Delta-cells on ethanol was decreased in comparison with that of wild-type cells, but the cells were still able to produce 3-methyl-1-butanol. However, drastic effects in fusel alcohol production were obtained in cells lacking BAT2. Although the constructed bat2Delta-single deletion strain and the bat1Deltabat2Delta-double deletion strain were still able to produce 3-methyl-1-butanol when grown on glucose, they were incapable of producing any 3-methyl-1-butanol when ethanol was the sole carbon source available. In the circumstances used, gene expression analysis revealed a strong upregulation of BAT2 gene activity in the wild type, when cells grew on ethanol as carbon source. Apparently, the carbon metabolism is able to influence the expression of BCAATs and interferes with the nitrogen metabolism. Furthermore, analysis of gene expression profiles shows that the expression of genes coding for other transaminases present in S. cerevisiae was influenced by the deletion of one or both BCAATs. Several transaminases were upregulated when a BCAAT was deleted. Strikingly, none of the known transaminases was significantly upregulated when BAT2 was deleted. Therefore we conclude that the expression of BAT2 is essential for 3-methyl-1-butanol formation on the non-fermentable carbon source, ethanol.

Authors+Show Affiliations

Department of Molecular Cell Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. s.a.schoondermark@bio.uu.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15851104

Citation

Schoondermark-Stolk, Sung A., et al. "Bat2p Is Essential in Saccharomyces Cerevisiae for Fusel Alcohol Production On the Non-fermentable Carbon Source Ethanol." FEMS Yeast Research, vol. 5, no. 8, 2005, pp. 757-66.
Schoondermark-Stolk SA, Tabernero M, Chapman J, et al. Bat2p is essential in Saccharomyces cerevisiae for fusel alcohol production on the non-fermentable carbon source ethanol. FEMS Yeast Res. 2005;5(8):757-66.
Schoondermark-Stolk, S. A., Tabernero, M., Chapman, J., Ter Schure, E. G., Verrips, C. T., Verkleij, A. J., & Boonstra, J. (2005). Bat2p is essential in Saccharomyces cerevisiae for fusel alcohol production on the non-fermentable carbon source ethanol. FEMS Yeast Research, 5(8), 757-66.
Schoondermark-Stolk SA, et al. Bat2p Is Essential in Saccharomyces Cerevisiae for Fusel Alcohol Production On the Non-fermentable Carbon Source Ethanol. FEMS Yeast Res. 2005;5(8):757-66. PubMed PMID: 15851104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bat2p is essential in Saccharomyces cerevisiae for fusel alcohol production on the non-fermentable carbon source ethanol. AU - Schoondermark-Stolk,Sung A, AU - Tabernero,Maria, AU - Chapman,John, AU - Ter Schure,Eelko G, AU - Verrips,C Theo, AU - Verkleij,Arie J, AU - Boonstra,Johannes, PY - 2004/11/08/received PY - 2005/01/10/revised PY - 2005/02/14/accepted PY - 2005/4/27/pubmed PY - 2005/12/21/medline PY - 2005/4/27/entrez SP - 757 EP - 66 JF - FEMS yeast research JO - FEMS Yeast Res VL - 5 IS - 8 N2 - Branched-chain amino acids (BCAAs) are key substrates in the formation of fusel alcohols, important flavour components in fermented foods. The first step in the catabolic BCAA degradation is a transaminase step, catalyzed by a branched-chain amino acid transaminase (BCAAT). Saccharomyces cerevisiae possesses a mitochondrial and a cytosolic BCAAT, Bat1p and Bat2p, respectively. In order to study the impact of the BCAATs on fusel alcohol production derived from the BCAA metabolism, S. cerevisiae BCAAT-deletion mutants were constructed. The BCAA l-leucine was exogenously supplied during cultivations with mutants of S. cerevisiae. BAT1 deletion is not essential for fusel alcohol production, neither under glucose nor under ethanol growth conditions. The 3-methyl-1-butanol production rate of bat1Delta-cells on ethanol was decreased in comparison with that of wild-type cells, but the cells were still able to produce 3-methyl-1-butanol. However, drastic effects in fusel alcohol production were obtained in cells lacking BAT2. Although the constructed bat2Delta-single deletion strain and the bat1Deltabat2Delta-double deletion strain were still able to produce 3-methyl-1-butanol when grown on glucose, they were incapable of producing any 3-methyl-1-butanol when ethanol was the sole carbon source available. In the circumstances used, gene expression analysis revealed a strong upregulation of BAT2 gene activity in the wild type, when cells grew on ethanol as carbon source. Apparently, the carbon metabolism is able to influence the expression of BCAATs and interferes with the nitrogen metabolism. Furthermore, analysis of gene expression profiles shows that the expression of genes coding for other transaminases present in S. cerevisiae was influenced by the deletion of one or both BCAATs. Several transaminases were upregulated when a BCAAT was deleted. Strikingly, none of the known transaminases was significantly upregulated when BAT2 was deleted. Therefore we conclude that the expression of BAT2 is essential for 3-methyl-1-butanol formation on the non-fermentable carbon source, ethanol. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/15851104/Bat2p_is_essential_in_Saccharomyces_cerevisiae_for_fusel_alcohol_production_on_the_non_fermentable_carbon_source_ethanol_ DB - PRIME DP - Unbound Medicine ER -