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Antioxidants in raspberry: on-line analysis links antioxidant activity to a diversity of individual metabolites.
J Agric Food Chem. 2005 May 04; 53(9):3313-20.JA

Abstract

The presence of antioxidant compounds can be considered as a quality parameter for edible fruit. In this paper, we studied the antioxidant compounds in raspberry (Rubus idaeus) fruits by high-performance liquid chromatography (HPLC) coupled to an on-line postcolumn antioxidant detection system. Both developmental and genetic factors were assessed by comparing fruits from a single cultivar of different ripening stages and by comparing ripe fruits of 14 raspberry cultivars, respectively. The HPLC-separated antioxidant compounds were identified using HPLC-photodiode array coupled to mass spectrometry (quadrupole time-of-flight tandem mass spectrometry), using a reference lock mass for determining accurate masses. The dominant antioxidants could be classified as anthocyanins, ellagitannins, and proanthocyanidin-like tannins. During fruit ripening, some anthocyanins were newly produced, while others, like cyanidin-3-glucoside, were already present early in fruit development. The level of tannins, both ellagitannins and proanthocyanidin-like tannins, was reduced strongly during fruit ripening. Among the 14 cultivars, major differences (>20-fold) were observed in the levels of pelagonidin type anthocyanins and some proanthocyanidin type tannins. The content of ellagitannins varied approximately 3-fold. The findings presented here suggest that the content of individual health-promoting compounds varies significantly in raspberry, due to both developmental and genetic factors. This information will assist in the future development and identification of raspberry lines with enhanced health-promoting properties.

Authors+Show Affiliations

Plant Research International, Postbus 16, 6700 AA Wageningen, The Netherlands. Jules.Beekwilder@wur.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15853365

Citation

Beekwilder, Jules, et al. "Antioxidants in Raspberry: On-line Analysis Links Antioxidant Activity to a Diversity of Individual Metabolites." Journal of Agricultural and Food Chemistry, vol. 53, no. 9, 2005, pp. 3313-20.
Beekwilder J, Jonker H, Meesters P, et al. Antioxidants in raspberry: on-line analysis links antioxidant activity to a diversity of individual metabolites. J Agric Food Chem. 2005;53(9):3313-20.
Beekwilder, J., Jonker, H., Meesters, P., Hall, R. D., van der Meer, I. M., & Ric de Vos, C. H. (2005). Antioxidants in raspberry: on-line analysis links antioxidant activity to a diversity of individual metabolites. Journal of Agricultural and Food Chemistry, 53(9), 3313-20.
Beekwilder J, et al. Antioxidants in Raspberry: On-line Analysis Links Antioxidant Activity to a Diversity of Individual Metabolites. J Agric Food Chem. 2005 May 4;53(9):3313-20. PubMed PMID: 15853365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidants in raspberry: on-line analysis links antioxidant activity to a diversity of individual metabolites. AU - Beekwilder,Jules, AU - Jonker,Harry, AU - Meesters,Patrick, AU - Hall,Robert D, AU - van der Meer,Ingrid M, AU - Ric de Vos,C H, PY - 2005/4/28/pubmed PY - 2005/6/29/medline PY - 2005/4/28/entrez SP - 3313 EP - 20 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 9 N2 - The presence of antioxidant compounds can be considered as a quality parameter for edible fruit. In this paper, we studied the antioxidant compounds in raspberry (Rubus idaeus) fruits by high-performance liquid chromatography (HPLC) coupled to an on-line postcolumn antioxidant detection system. Both developmental and genetic factors were assessed by comparing fruits from a single cultivar of different ripening stages and by comparing ripe fruits of 14 raspberry cultivars, respectively. The HPLC-separated antioxidant compounds were identified using HPLC-photodiode array coupled to mass spectrometry (quadrupole time-of-flight tandem mass spectrometry), using a reference lock mass for determining accurate masses. The dominant antioxidants could be classified as anthocyanins, ellagitannins, and proanthocyanidin-like tannins. During fruit ripening, some anthocyanins were newly produced, while others, like cyanidin-3-glucoside, were already present early in fruit development. The level of tannins, both ellagitannins and proanthocyanidin-like tannins, was reduced strongly during fruit ripening. Among the 14 cultivars, major differences (>20-fold) were observed in the levels of pelagonidin type anthocyanins and some proanthocyanidin type tannins. The content of ellagitannins varied approximately 3-fold. The findings presented here suggest that the content of individual health-promoting compounds varies significantly in raspberry, due to both developmental and genetic factors. This information will assist in the future development and identification of raspberry lines with enhanced health-promoting properties. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15853365/Antioxidants_in_raspberry:_on_line_analysis_links_antioxidant_activity_to_a_diversity_of_individual_metabolites_ L2 - https://doi.org/10.1021/jf047880b DB - PRIME DP - Unbound Medicine ER -