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Compensatory role of PspA, a member of the phage shock protein operon, in rpoE mutant Salmonella enterica serovar Typhimurium.
Mol Microbiol. 2005 May; 56(4):1004-16.MM

Abstract

Sigma(E) is an alternative sigma factor that responds to and ameliorates extracytoplasmic stress. In Salmonella enterica serovar Typhimurium (S. Typhimurium), sigma(E) is required for oxidative stress resistance, stationary-phase survival and virulence in mice. Microarray analysis of stationary-phase gene expression in rpoE mutant bacteria revealed a dramatic increase in expression of pspA, a member of the phage shock protein (psp) operon. The psp operon can be induced by filamentous bacteriophages or by perturbations of protein secretion, and is believed to facilitate the maintenance of proton motive force (PMF). We hypothesized that increased pspA expression may represent a compensatory response to the loss of sigma(E) function. Increased pspA expression was confirmed in rpoE mutant Salmonella and also observed in a mutant lacking the F(1)F(0) ATPase. Alternatively, expression of pspA could be induced by exposure to CCCP, a protonophore that disrupts PMF. An rpoE pspA double mutant strain was found to have a stationary-phase survival defect more pronounced than that of isogenic strains harbouring single mutations. The double mutant strains were also more susceptible to killing by CCCP or by a bactericidal/permeability-increasing protein (BPI)-derived anti-microbial peptide. Using fluorescence ratio imaging, differences were observed in the Deltapsi of wild-type and rpoE or pspA mutant bacteria. These findings suggest that pspA expression in S. Typhimurium is induced by alterations in PMF and a functional sigma(E) regulon is essential for the maintenance of PMF.

Authors+Show Affiliations

Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15853886

Citation

Becker, Lynne A., et al. "Compensatory Role of PspA, a Member of the Phage Shock Protein Operon, in rpoE Mutant Salmonella Enterica Serovar Typhimurium." Molecular Microbiology, vol. 56, no. 4, 2005, pp. 1004-16.
Becker LA, Bang IS, Crouch ML, et al. Compensatory role of PspA, a member of the phage shock protein operon, in rpoE mutant Salmonella enterica serovar Typhimurium. Mol Microbiol. 2005;56(4):1004-16.
Becker, L. A., Bang, I. S., Crouch, M. L., & Fang, F. C. (2005). Compensatory role of PspA, a member of the phage shock protein operon, in rpoE mutant Salmonella enterica serovar Typhimurium. Molecular Microbiology, 56(4), 1004-16.
Becker LA, et al. Compensatory Role of PspA, a Member of the Phage Shock Protein Operon, in rpoE Mutant Salmonella Enterica Serovar Typhimurium. Mol Microbiol. 2005;56(4):1004-16. PubMed PMID: 15853886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compensatory role of PspA, a member of the phage shock protein operon, in rpoE mutant Salmonella enterica serovar Typhimurium. AU - Becker,Lynne A, AU - Bang,Iel-Soo, AU - Crouch,Marie-Laure, AU - Fang,Ferric C, PY - 2005/4/28/pubmed PY - 2005/9/21/medline PY - 2005/4/28/entrez SP - 1004 EP - 16 JF - Molecular microbiology JO - Mol Microbiol VL - 56 IS - 4 N2 - Sigma(E) is an alternative sigma factor that responds to and ameliorates extracytoplasmic stress. In Salmonella enterica serovar Typhimurium (S. Typhimurium), sigma(E) is required for oxidative stress resistance, stationary-phase survival and virulence in mice. Microarray analysis of stationary-phase gene expression in rpoE mutant bacteria revealed a dramatic increase in expression of pspA, a member of the phage shock protein (psp) operon. The psp operon can be induced by filamentous bacteriophages or by perturbations of protein secretion, and is believed to facilitate the maintenance of proton motive force (PMF). We hypothesized that increased pspA expression may represent a compensatory response to the loss of sigma(E) function. Increased pspA expression was confirmed in rpoE mutant Salmonella and also observed in a mutant lacking the F(1)F(0) ATPase. Alternatively, expression of pspA could be induced by exposure to CCCP, a protonophore that disrupts PMF. An rpoE pspA double mutant strain was found to have a stationary-phase survival defect more pronounced than that of isogenic strains harbouring single mutations. The double mutant strains were also more susceptible to killing by CCCP or by a bactericidal/permeability-increasing protein (BPI)-derived anti-microbial peptide. Using fluorescence ratio imaging, differences were observed in the Deltapsi of wild-type and rpoE or pspA mutant bacteria. These findings suggest that pspA expression in S. Typhimurium is induced by alterations in PMF and a functional sigma(E) regulon is essential for the maintenance of PMF. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/15853886/Compensatory_role_of_PspA_a_member_of_the_phage_shock_protein_operon_in_rpoE_mutant_Salmonella_enterica_serovar_Typhimurium_ L2 - https://doi.org/10.1111/j.1365-2958.2005.04604.x DB - PRIME DP - Unbound Medicine ER -