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Longitudinal analysis of metalloproteinases, tissue inhibitors of metalloproteinases and clinical parameters in gingival crevicular fluid from periodontitis-affected patients.
J Periodontal Res. 2005 Jun; 40(3):199-207.JP

Abstract

BACKGROUND

The aim of this work was to improve the assessment of the periodontal disease status through measurements of extracellular matrix metalloproteinases (MMPs) and their tissular inhibitors (TIMPs) in the gingival crevicular fluid from patients diagnosed with chronic periodontitis.

METHODS

Gingival crevicular fluid samples from patients (n = 13) were taken from 60 sites initially, and from 51 and 41 sites, respectively, 3 and 6 months after scaling and root planing. Gingival crevicular fluid samples were also taken from healthy subjects (n = 11, 24 sites). The presence of MMP-9 and MMP-8 was assessed by zymography and immunowestern blotting, respectively. The actual MMP activity (gelatinase and collagenase) was measured using the fluorogenic substrate assay. TIMP-1 and -2 levels were measured by immunodot blot.

RESULTS

The fluorogenic substrate assay determinations showed higher MMP activity in sites with probing depth > or = 4 mm, with significant reduction post-treatment. Gelatinase activity followed by zymography consisted mainly of MMP-9. A different pattern of MMP-8 in control and patient sites was found. Controls only showed species of a partially active form (69 kDa), whereas patient sites showed a high frequency of the active form (56 kDa), and in some cases the latent form (85 kDa) was also observed. The active form reduced its frequency in sites with probing depth > or = 4 mm. TIMP-1 and -2 levels in patients were significantly lower than in controls, and after treatment the recovery of TIMP-1 level similar to control was observed.

CONCLUSION

Significant correlations between the severity of the periodontal disease and the actual MMP activity, the active form of MMP-8 and the low level of both TIMP-1 and TIMP-2 were found.

Authors+Show Affiliations

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15853964

Citation

Pozo, P, et al. "Longitudinal Analysis of Metalloproteinases, Tissue Inhibitors of Metalloproteinases and Clinical Parameters in Gingival Crevicular Fluid From Periodontitis-affected Patients." Journal of Periodontal Research, vol. 40, no. 3, 2005, pp. 199-207.
Pozo P, Valenzuela MA, Melej C, et al. Longitudinal analysis of metalloproteinases, tissue inhibitors of metalloproteinases and clinical parameters in gingival crevicular fluid from periodontitis-affected patients. J Periodontal Res. 2005;40(3):199-207.
Pozo, P., Valenzuela, M. A., Melej, C., Zaldívar, M., Puente, J., Martínez, B., & Gamonal, J. (2005). Longitudinal analysis of metalloproteinases, tissue inhibitors of metalloproteinases and clinical parameters in gingival crevicular fluid from periodontitis-affected patients. Journal of Periodontal Research, 40(3), 199-207.
Pozo P, et al. Longitudinal Analysis of Metalloproteinases, Tissue Inhibitors of Metalloproteinases and Clinical Parameters in Gingival Crevicular Fluid From Periodontitis-affected Patients. J Periodontal Res. 2005;40(3):199-207. PubMed PMID: 15853964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Longitudinal analysis of metalloproteinases, tissue inhibitors of metalloproteinases and clinical parameters in gingival crevicular fluid from periodontitis-affected patients. AU - Pozo,P, AU - Valenzuela,M A, AU - Melej,C, AU - Zaldívar,M, AU - Puente,J, AU - Martínez,B, AU - Gamonal,J, PY - 2005/4/28/pubmed PY - 2005/8/23/medline PY - 2005/4/28/entrez SP - 199 EP - 207 JF - Journal of periodontal research JO - J Periodontal Res VL - 40 IS - 3 N2 - BACKGROUND: The aim of this work was to improve the assessment of the periodontal disease status through measurements of extracellular matrix metalloproteinases (MMPs) and their tissular inhibitors (TIMPs) in the gingival crevicular fluid from patients diagnosed with chronic periodontitis. METHODS: Gingival crevicular fluid samples from patients (n = 13) were taken from 60 sites initially, and from 51 and 41 sites, respectively, 3 and 6 months after scaling and root planing. Gingival crevicular fluid samples were also taken from healthy subjects (n = 11, 24 sites). The presence of MMP-9 and MMP-8 was assessed by zymography and immunowestern blotting, respectively. The actual MMP activity (gelatinase and collagenase) was measured using the fluorogenic substrate assay. TIMP-1 and -2 levels were measured by immunodot blot. RESULTS: The fluorogenic substrate assay determinations showed higher MMP activity in sites with probing depth > or = 4 mm, with significant reduction post-treatment. Gelatinase activity followed by zymography consisted mainly of MMP-9. A different pattern of MMP-8 in control and patient sites was found. Controls only showed species of a partially active form (69 kDa), whereas patient sites showed a high frequency of the active form (56 kDa), and in some cases the latent form (85 kDa) was also observed. The active form reduced its frequency in sites with probing depth > or = 4 mm. TIMP-1 and -2 levels in patients were significantly lower than in controls, and after treatment the recovery of TIMP-1 level similar to control was observed. CONCLUSION: Significant correlations between the severity of the periodontal disease and the actual MMP activity, the active form of MMP-8 and the low level of both TIMP-1 and TIMP-2 were found. SN - 0022-3484 UR - https://www.unboundmedicine.com/medline/citation/15853964/Longitudinal_analysis_of_metalloproteinases_tissue_inhibitors_of_metalloproteinases_and_clinical_parameters_in_gingival_crevicular_fluid_from_periodontitis_affected_patients_ L2 - https://doi.org/10.1111/j.1600-0765.2005.00786.x DB - PRIME DP - Unbound Medicine ER -