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Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)-mediated enhancement of dengue virus infection is independent of DC-SIGN internalization signals.
J Biol Chem. 2005 Jun 24; 280(25):23698-708.JB

Abstract

Dengue virus (DV) is a mosquito-borne flavivirus that causes hemorrhagic fever in humans. In the natural infection, DV is introduced into human skin by an infected mosquito vector where it is believed to target immature dendritic cells (DCs) and Langerhans cells (LCs). We found that DV productively infects DCs but not LCs. We show here that the interactions between DV E protein, the sole mannosylated glycoprotein present on DV particles, and the C-type lectin dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) are essential for DV infection of DCs. Binding of mannosylated N-glycans on DV E protein to DC-SIGN triggers a rapid and efficient internalization of the viral glycoprotein. However, we observed that endocytosis-defective DC-SIGN molecules allow efficient DV replication, indicating that DC-SIGN endocytosis is dispensable for the internalization step in DV entry. Together, these results argue in favor of a mechanism by which DC-SIGN enhances DV entry and infection in cis. We propose that DC-SIGN concentrates mosquito-derived DV particles at the cell surface to allow efficient interaction with an as yet unidentified entry factor that is ultimately responsible for DV internalization and pH-dependent fusion into DCs.

Authors+Show Affiliations

Lozach PY
Unité d'Immunologie Virale, Institut Pasteur Paris, 25-28, rue du Dr Roux, 75724 Paris Cedex 15, France.
Burleigh L
No affiliation info available
Staropoli I
No affiliation info available
Navarro-Sanchez E
No affiliation info available
Harriague J
No affiliation info available
Virelizier JL
No affiliation info available
Rey FA
No affiliation info available
Desprès P
No affiliation info available
Arenzana-Seisdedos F
No affiliation info available
Amara A
No affiliation info available

MeSH

Base SequenceCell Adhesion MoleculesCell LineDNA PrimersDengueDengue VirusFlow CytometryHumansLectins, C-TypeReceptors, Cell SurfaceVirion

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15855154

Citation

Lozach, Pierre-Yves, et al. "Dendritic Cell-specific Intercellular Adhesion Molecule 3-grabbing Non-integrin (DC-SIGN)-mediated Enhancement of Dengue Virus Infection Is Independent of DC-SIGN Internalization Signals." The Journal of Biological Chemistry, vol. 280, no. 25, 2005, pp. 23698-708.
Lozach PY, Burleigh L, Staropoli I, et al. Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)-mediated enhancement of dengue virus infection is independent of DC-SIGN internalization signals. J Biol Chem. 2005;280(25):23698-708.
Lozach, P. Y., Burleigh, L., Staropoli, I., Navarro-Sanchez, E., Harriague, J., Virelizier, J. L., Rey, F. A., Desprès, P., Arenzana-Seisdedos, F., & Amara, A. (2005). Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)-mediated enhancement of dengue virus infection is independent of DC-SIGN internalization signals. The Journal of Biological Chemistry, 280(25), 23698-708.
Lozach PY, et al. Dendritic Cell-specific Intercellular Adhesion Molecule 3-grabbing Non-integrin (DC-SIGN)-mediated Enhancement of Dengue Virus Infection Is Independent of DC-SIGN Internalization Signals. J Biol Chem. 2005 Jun 24;280(25):23698-708. PubMed PMID: 15855154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)-mediated enhancement of dengue virus infection is independent of DC-SIGN internalization signals. AU - Lozach,Pierre-Yves, AU - Burleigh,Laura, AU - Staropoli,Isabelle, AU - Navarro-Sanchez,Erika, AU - Harriague,Julie, AU - Virelizier,Jean-Louis, AU - Rey,Felix A, AU - Desprès,Philippe, AU - Arenzana-Seisdedos,Fernando, AU - Amara,Ali, Y1 - 2005/04/26/ PY - 2005/4/28/pubmed PY - 2005/9/30/medline PY - 2005/4/28/entrez SP - 23698 EP - 708 JF - The Journal of biological chemistry JO - J Biol Chem VL - 280 IS - 25 N2 - Dengue virus (DV) is a mosquito-borne flavivirus that causes hemorrhagic fever in humans. In the natural infection, DV is introduced into human skin by an infected mosquito vector where it is believed to target immature dendritic cells (DCs) and Langerhans cells (LCs). We found that DV productively infects DCs but not LCs. We show here that the interactions between DV E protein, the sole mannosylated glycoprotein present on DV particles, and the C-type lectin dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) are essential for DV infection of DCs. Binding of mannosylated N-glycans on DV E protein to DC-SIGN triggers a rapid and efficient internalization of the viral glycoprotein. However, we observed that endocytosis-defective DC-SIGN molecules allow efficient DV replication, indicating that DC-SIGN endocytosis is dispensable for the internalization step in DV entry. Together, these results argue in favor of a mechanism by which DC-SIGN enhances DV entry and infection in cis. We propose that DC-SIGN concentrates mosquito-derived DV particles at the cell surface to allow efficient interaction with an as yet unidentified entry factor that is ultimately responsible for DV internalization and pH-dependent fusion into DCs. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/15855154/Dendritic_cell_specific_intercellular_adhesion_molecule_3_grabbing_non_integrin__DC_SIGN__mediated_enhancement_of_dengue_virus_infection_is_independent_of_DC_SIGN_internalization_signals_ DB - PRIME DP - Unbound Medicine ER -