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Alterations of lipids and apolipoprotein CIII in very low density lipoprotein subspecies in type 2 diabetes.
Diabetologia. 2005 Jun; 48(6):1207-15.D

Abstract

AIMS/HYPOTHESIS

Very low density lipoprotein (VLDL) particles are heterogeneous, comprising two main subspecies, VLDL 1 (Sf 60-400) and VLDL 2 (Sf 20-60). The aim of the study was to examine the distribution and composition of VLDL subspecies in type 2 diabetes.

SUBJECTS, MATERIALS AND METHODS

We studied the composition and concentration of triglyceride-rich lipoproteins (TRLs) in 217 type 2 diabetic patients and 93 control subjects between 50 and 75 years of age. Lipoprotein subspecies were separated by density-gradient ultracentrifugation. Apolipoprotein (apo) CIII and apo E in plasma and apo CIII in TRL subspecies were measured by nephelometry and apo CII in serum by a commercial kit using a single radial immunodiffusion method.

RESULTS

The concentrations of VLDL 1, VLDL 2 and intermediate density lipoprotein were significantly increased in type 2 diabetes subjects, the change being most marked for VLDL 1. There was a strong linear correlation between VLDL 1 triglycerides and plasma triglycerides in both groups (r = 0.879, p < 0.001 and r = 0.899, p < 0.001). Diabetic subjects had markedly higher plasma ratios of apo CII:apo CIII and apo CIII:apo E. Despite elevated plasma apo CIII, type 2 diabetic subjects had a relative deficiency of apo CIII in all TRL subspecies, suggesting profound disturbances of apo CIII metabolism.

CONCLUSIONS/INTERPRETATION

The elevation of VLDL 1 triglycerides is the major determinant of plasma triglyceride concentration in normal subjects and in type 2 diabetic individuals. Both apo CIII and apo E metabolism are disturbed in type 2 diabetes.

Authors+Show Affiliations

Department of Medicine, Division of Cardiology, Helsinki University Hospital and Biomedicum, Haartmaninkatu 8, 00029, Helsinki, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15864534

Citation

Hiukka, Anne, et al. "Alterations of Lipids and Apolipoprotein CIII in Very Low Density Lipoprotein Subspecies in Type 2 Diabetes." Diabetologia, vol. 48, no. 6, 2005, pp. 1207-15.
Hiukka A, Fruchart-Najib J, Leinonen E, et al. Alterations of lipids and apolipoprotein CIII in very low density lipoprotein subspecies in type 2 diabetes. Diabetologia. 2005;48(6):1207-15.
Hiukka, A., Fruchart-Najib, J., Leinonen, E., Hilden, H., Fruchart, J. C., & Taskinen, M. R. (2005). Alterations of lipids and apolipoprotein CIII in very low density lipoprotein subspecies in type 2 diabetes. Diabetologia, 48(6), 1207-15.
Hiukka A, et al. Alterations of Lipids and Apolipoprotein CIII in Very Low Density Lipoprotein Subspecies in Type 2 Diabetes. Diabetologia. 2005;48(6):1207-15. PubMed PMID: 15864534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alterations of lipids and apolipoprotein CIII in very low density lipoprotein subspecies in type 2 diabetes. AU - Hiukka,Anne, AU - Fruchart-Najib,Jamila, AU - Leinonen,Eeva, AU - Hilden,Hannele, AU - Fruchart,Jean-Charles, AU - Taskinen,Marja-Riitta, Y1 - 2005/04/30/ PY - 2004/09/22/received PY - 2005/01/27/accepted PY - 2005/5/3/pubmed PY - 2005/9/22/medline PY - 2005/5/3/entrez SP - 1207 EP - 15 JF - Diabetologia JO - Diabetologia VL - 48 IS - 6 N2 - AIMS/HYPOTHESIS: Very low density lipoprotein (VLDL) particles are heterogeneous, comprising two main subspecies, VLDL 1 (Sf 60-400) and VLDL 2 (Sf 20-60). The aim of the study was to examine the distribution and composition of VLDL subspecies in type 2 diabetes. SUBJECTS, MATERIALS AND METHODS: We studied the composition and concentration of triglyceride-rich lipoproteins (TRLs) in 217 type 2 diabetic patients and 93 control subjects between 50 and 75 years of age. Lipoprotein subspecies were separated by density-gradient ultracentrifugation. Apolipoprotein (apo) CIII and apo E in plasma and apo CIII in TRL subspecies were measured by nephelometry and apo CII in serum by a commercial kit using a single radial immunodiffusion method. RESULTS: The concentrations of VLDL 1, VLDL 2 and intermediate density lipoprotein were significantly increased in type 2 diabetes subjects, the change being most marked for VLDL 1. There was a strong linear correlation between VLDL 1 triglycerides and plasma triglycerides in both groups (r = 0.879, p < 0.001 and r = 0.899, p < 0.001). Diabetic subjects had markedly higher plasma ratios of apo CII:apo CIII and apo CIII:apo E. Despite elevated plasma apo CIII, type 2 diabetic subjects had a relative deficiency of apo CIII in all TRL subspecies, suggesting profound disturbances of apo CIII metabolism. CONCLUSIONS/INTERPRETATION: The elevation of VLDL 1 triglycerides is the major determinant of plasma triglyceride concentration in normal subjects and in type 2 diabetic individuals. Both apo CIII and apo E metabolism are disturbed in type 2 diabetes. SN - 0012-186X UR - https://www.unboundmedicine.com/medline/citation/15864534/Alterations_of_lipids_and_apolipoprotein_CIII_in_very_low_density_lipoprotein_subspecies_in_type_2_diabetes_ L2 - https://doi.org/10.1007/s00125-005-1753-z DB - PRIME DP - Unbound Medicine ER -