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Combined flow cytometry determination of S-phase fraction and DNA ploidy is an independent prognostic factor in node-negative invasive breast carcinoma: analysis of a series of 271 patients with stage I and II breast cancer.
Breast Cancer Res Treat. 2005 May; 91(1):61-71.BC

Abstract

PURPOSE

To assess the significance of S-phase fraction (SPF) and DNA ploidy evaluated by DNA flow cytometry as prognostic markers in stage I or II breast cancer.

PATIENTS AND METHODS

A series of 271 patients, treated by surgery, radiotherapy +/- systemic therapy was analyzed (median follow up: 64 months). Standardized flow cytometry cell preparation from frozen samples and consensus rules for data interpretation were followed. Three SPF classes were defined on the basis of tertiles after adjustment for ploidy. Four groups were defined based on combinations of DNA ploidy (DIP: diploid; ANEUP: aneuploid) and SPF: DIP and low SPF (DL, n=37), DIP and medium or high SPF (DMH, n=76), ANEUP and low SPF (AL, n=24), ANEUP and medium or high SPF (AMH, n=68). Local control rate (LCR), disease-free survival (DFS), metastasis-free survival (MFS), and overall survival (OS) were correlated with DNA ploidy, SPF, DL to AMH groups, T and N stages, SBR grading, age, and hormonal status on univariate and multivariate analysis (Cox model).

RESULTS

On univariate analysis, DFS and LCR were higher for DIP tumours. High SPF values were associated with shorter DFS. LCR, MFS, DFS, and OS rates were significantly different with an increasingly poorer prognosis from DL to AMH. On multivariate analysis, groups DL to AMH, histological node involvement and T stage were independently associated with MFS, and DFS. In N- patients, DL to AMH remained independent for MFS and DFS. For SBR III tumours, MFS and OS were significantly different in DL to AMH groups. These results strongly support the use of combined evaluation of DNA ploidy and SPF as independent parameters in clinical trials for N- stage I and II breast cancer.

Authors+Show Affiliations

Service d'Oncologie Radiothérapie, Université Paris, France. moureaul@marseille.fnclcc.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15868432

Citation

Moureau-Zabotto, L, et al. "Combined Flow Cytometry Determination of S-phase Fraction and DNA Ploidy Is an Independent Prognostic Factor in Node-negative Invasive Breast Carcinoma: Analysis of a Series of 271 Patients With Stage I and II Breast Cancer." Breast Cancer Research and Treatment, vol. 91, no. 1, 2005, pp. 61-71.
Moureau-Zabotto L, Bouchet C, Cesari D, et al. Combined flow cytometry determination of S-phase fraction and DNA ploidy is an independent prognostic factor in node-negative invasive breast carcinoma: analysis of a series of 271 patients with stage I and II breast cancer. Breast Cancer Res Treat. 2005;91(1):61-71.
Moureau-Zabotto, L., Bouchet, C., Cesari, D., Uzan, S., Lefranc, J. P., Antoine, M., Genestie, C., Deniaud-Alexandre, E., Bernaudin, J. F., Touboul, E., & Fleury-Feith, J. (2005). Combined flow cytometry determination of S-phase fraction and DNA ploidy is an independent prognostic factor in node-negative invasive breast carcinoma: analysis of a series of 271 patients with stage I and II breast cancer. Breast Cancer Research and Treatment, 91(1), 61-71.
Moureau-Zabotto L, et al. Combined Flow Cytometry Determination of S-phase Fraction and DNA Ploidy Is an Independent Prognostic Factor in Node-negative Invasive Breast Carcinoma: Analysis of a Series of 271 Patients With Stage I and II Breast Cancer. Breast Cancer Res Treat. 2005;91(1):61-71. PubMed PMID: 15868432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined flow cytometry determination of S-phase fraction and DNA ploidy is an independent prognostic factor in node-negative invasive breast carcinoma: analysis of a series of 271 patients with stage I and II breast cancer. AU - Moureau-Zabotto,L, AU - Bouchet,C, AU - Cesari,D, AU - Uzan,S, AU - Lefranc,J-P, AU - Antoine,M, AU - Genestie,C, AU - Deniaud-Alexandre,E, AU - Bernaudin,J-F, AU - Touboul,E, AU - Fleury-Feith,J, PY - 2005/5/4/pubmed PY - 2005/7/13/medline PY - 2005/5/4/entrez SP - 61 EP - 71 JF - Breast cancer research and treatment JO - Breast Cancer Res Treat VL - 91 IS - 1 N2 - PURPOSE: To assess the significance of S-phase fraction (SPF) and DNA ploidy evaluated by DNA flow cytometry as prognostic markers in stage I or II breast cancer. PATIENTS AND METHODS: A series of 271 patients, treated by surgery, radiotherapy +/- systemic therapy was analyzed (median follow up: 64 months). Standardized flow cytometry cell preparation from frozen samples and consensus rules for data interpretation were followed. Three SPF classes were defined on the basis of tertiles after adjustment for ploidy. Four groups were defined based on combinations of DNA ploidy (DIP: diploid; ANEUP: aneuploid) and SPF: DIP and low SPF (DL, n=37), DIP and medium or high SPF (DMH, n=76), ANEUP and low SPF (AL, n=24), ANEUP and medium or high SPF (AMH, n=68). Local control rate (LCR), disease-free survival (DFS), metastasis-free survival (MFS), and overall survival (OS) were correlated with DNA ploidy, SPF, DL to AMH groups, T and N stages, SBR grading, age, and hormonal status on univariate and multivariate analysis (Cox model). RESULTS: On univariate analysis, DFS and LCR were higher for DIP tumours. High SPF values were associated with shorter DFS. LCR, MFS, DFS, and OS rates were significantly different with an increasingly poorer prognosis from DL to AMH. On multivariate analysis, groups DL to AMH, histological node involvement and T stage were independently associated with MFS, and DFS. In N- patients, DL to AMH remained independent for MFS and DFS. For SBR III tumours, MFS and OS were significantly different in DL to AMH groups. These results strongly support the use of combined evaluation of DNA ploidy and SPF as independent parameters in clinical trials for N- stage I and II breast cancer. SN - 0167-6806 UR - https://www.unboundmedicine.com/medline/citation/15868432/Combined_flow_cytometry_determination_of_S_phase_fraction_and_DNA_ploidy_is_an_independent_prognostic_factor_in_node_negative_invasive_breast_carcinoma:_analysis_of_a_series_of_271_patients_with_stage_I_and_II_breast_cancer_ L2 - https://doi.org/10.1007/s10549-004-7047-1 DB - PRIME DP - Unbound Medicine ER -