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Epidermal growth factor receptor signaling regulates goblet cell production after small bowel resection.
J Pediatr Surg. 2005 Jan; 40(1):92-7.JP

Abstract

BACKGROUND/PURPOSE

Intestinal adaptation is a compensatory response to massive small bowel loss in which there are increased numbers of absorptive enterocytes. However, the generation of secretory epithelial cell subtypes in this process has not been investigated. The purpose of this study was to examine the adaptive changes of several small intestinal cell lineage changes in response to massive small bowel resection (SBR).

METHODS

A 75% SBR or sham operation was performed on male Sprague-Dawley rats. On postoperative day 7, the remnant ileum was harvested and immunohistochemical staining for goblet, Paneth, and enteroendocrine cells was performed. Cell subtypes were evaluated as cells per micrometer of villus/crypt length and compared among operations.

RESULTS

A significant increase in goblet cell density occurred after SBR. Intestinal resection did not alter the number of Paneth and enteroendocrine cells. In additional experiments, inhibition of epidermal growth factor receptor signaling was associated with a diminished goblet cell density.

CONCLUSIONS

The adaptive response of the intestine to massive bowel loss results in an expansion of the goblet cell population in addition to greater numbers of absorptive enterocytes. Although the mechanism and purpose for selective expansion of these stem cell-derived lineages are not presently known, epidermal growth factor receptor signaling appears to be a common pathway.

Authors+Show Affiliations

Division of Pediatric and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229-3039, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15868565

Citation

Jarboe, Marcus D., et al. "Epidermal Growth Factor Receptor Signaling Regulates Goblet Cell Production After Small Bowel Resection." Journal of Pediatric Surgery, vol. 40, no. 1, 2005, pp. 92-7.
Jarboe MD, Juno RJ, Stehr W, et al. Epidermal growth factor receptor signaling regulates goblet cell production after small bowel resection. J Pediatr Surg. 2005;40(1):92-7.
Jarboe, M. D., Juno, R. J., Stehr, W., Bernal, N. P., Profitt, S., Erwin, C. R., & Warner, B. W. (2005). Epidermal growth factor receptor signaling regulates goblet cell production after small bowel resection. Journal of Pediatric Surgery, 40(1), 92-7.
Jarboe MD, et al. Epidermal Growth Factor Receptor Signaling Regulates Goblet Cell Production After Small Bowel Resection. J Pediatr Surg. 2005;40(1):92-7. PubMed PMID: 15868565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidermal growth factor receptor signaling regulates goblet cell production after small bowel resection. AU - Jarboe,Marcus D, AU - Juno,Russell J, AU - Stehr,Wolfgang, AU - Bernal,Nicole P, AU - Profitt,Sherri, AU - Erwin,Christopher R, AU - Warner,Brad W, PY - 2005/5/4/pubmed PY - 2006/6/10/medline PY - 2005/5/4/entrez SP - 92 EP - 7 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 40 IS - 1 N2 - BACKGROUND/PURPOSE: Intestinal adaptation is a compensatory response to massive small bowel loss in which there are increased numbers of absorptive enterocytes. However, the generation of secretory epithelial cell subtypes in this process has not been investigated. The purpose of this study was to examine the adaptive changes of several small intestinal cell lineage changes in response to massive small bowel resection (SBR). METHODS: A 75% SBR or sham operation was performed on male Sprague-Dawley rats. On postoperative day 7, the remnant ileum was harvested and immunohistochemical staining for goblet, Paneth, and enteroendocrine cells was performed. Cell subtypes were evaluated as cells per micrometer of villus/crypt length and compared among operations. RESULTS: A significant increase in goblet cell density occurred after SBR. Intestinal resection did not alter the number of Paneth and enteroendocrine cells. In additional experiments, inhibition of epidermal growth factor receptor signaling was associated with a diminished goblet cell density. CONCLUSIONS: The adaptive response of the intestine to massive bowel loss results in an expansion of the goblet cell population in addition to greater numbers of absorptive enterocytes. Although the mechanism and purpose for selective expansion of these stem cell-derived lineages are not presently known, epidermal growth factor receptor signaling appears to be a common pathway. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/15868565/Epidermal_growth_factor_receptor_signaling_regulates_goblet_cell_production_after_small_bowel_resection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022346804006128 DB - PRIME DP - Unbound Medicine ER -