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The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks.
Mol Cell Biol. 2005 May; 25(10):3934-44.MC

Abstract

Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity. Nonhomologous end joining (NHEJ) is the major cellular repair pathway that eliminates chromosome DSBs. Here we report our genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors. Deletion of RSC30 gene or the C-terminal truncation of RSC8 impairs NHEJ of a chromosome DSB created by HO endonuclease in vivo. rsc30Delta maintains a robust level of homologous recombination and the damage-induced cell cycle checkpoints. By chromatin immunoprecipitation, we show recruitment of RSC to a chromosome DSB with kinetics congruent with its involvement in NHEJ. Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p. The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ.

Authors+Show Affiliations

Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15870268

Citation

Shim, Eun Yong, et al. "The Yeast Chromatin Remodeler RSC Complex Facilitates End Joining Repair of DNA Double-strand Breaks." Molecular and Cellular Biology, vol. 25, no. 10, 2005, pp. 3934-44.
Shim EY, Ma JL, Oum JH, et al. The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol. 2005;25(10):3934-44.
Shim, E. Y., Ma, J. L., Oum, J. H., Yanez, Y., & Lee, S. E. (2005). The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Molecular and Cellular Biology, 25(10), 3934-44.
Shim EY, et al. The Yeast Chromatin Remodeler RSC Complex Facilitates End Joining Repair of DNA Double-strand Breaks. Mol Cell Biol. 2005;25(10):3934-44. PubMed PMID: 15870268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. AU - Shim,Eun Yong, AU - Ma,Jia-Lin, AU - Oum,Ji-Hyun, AU - Yanez,Yvonne, AU - Lee,Sang Eun, PY - 2005/5/5/pubmed PY - 2005/6/15/medline PY - 2005/5/5/entrez SP - 3934 EP - 44 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 25 IS - 10 N2 - Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity. Nonhomologous end joining (NHEJ) is the major cellular repair pathway that eliminates chromosome DSBs. Here we report our genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors. Deletion of RSC30 gene or the C-terminal truncation of RSC8 impairs NHEJ of a chromosome DSB created by HO endonuclease in vivo. rsc30Delta maintains a robust level of homologous recombination and the damage-induced cell cycle checkpoints. By chromatin immunoprecipitation, we show recruitment of RSC to a chromosome DSB with kinetics congruent with its involvement in NHEJ. Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p. The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/15870268/The_yeast_chromatin_remodeler_RSC_complex_facilitates_end_joining_repair_of_DNA_double_strand_breaks_ L2 - https://journals.asm.org/doi/10.1128/MCB.25.10.3934-3944.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -