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New resistance mechanism in Helicoverpa armigera threatens transgenic crops expressing Bacillus thuringiensis Cry1Ac toxin.
Appl Environ Microbiol. 2005 May; 71(5):2558-63.AE

Abstract

In Australia, the cotton bollworm, Helicoverpa armigera, has a long history of resistance to conventional insecticides. Transgenic cotton (expressing the Bacillus thuringiensis toxin Cry1Ac) has been grown for H. armigera control since 1996. It is demonstrated here that a population of Australian H. armigera has developed resistance to Cry1Ac toxin (275-fold). Some 70% of resistant H. armigera larvae were able to survive on Cry1Ac transgenic cotton (Ingard) The resistance phenotype is inherited as an autosomal semidominant trait. Resistance was associated with elevated esterase levels, which cosegregated with resistance. In vitro studies employing surface plasmon resonance technology and other biochemical techniques demonstrated that resistant strain esterase could bind to Cry1Ac protoxin and activated toxin. In vivo studies showed that Cry1Ac-resistant larvae fed Cy1Ac transgenic cotton or Cry1Ac-treated artificial diet had lower esterase activity than non-Cry1Ac-fed larvae. A resistance mechanism in which esterase sequesters Cry1Ac is proposed.

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Authors+Show Affiliations

Gunning RV
New South Wales Department of Primary Industries, Tamworth, NSW, Australia 2340. robin.gunning@agric.nsw.gov.au
Dang HT
No affiliation info available
Kemp FC
No affiliation info available
Nicholson IC
No affiliation info available
Moores GD
No affiliation info available

MeSH

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsBacterial ToxinsBiological AssayCrops, AgriculturalEndotoxinsEsterasesHemolysin ProteinsInsecticide ResistanceMothsPest Control, BiologicalPlants, Genetically Modified

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15870346

Citation

Gunning, Robin V., et al. "New Resistance Mechanism in Helicoverpa Armigera Threatens Transgenic Crops Expressing Bacillus Thuringiensis Cry1Ac Toxin." Applied and Environmental Microbiology, vol. 71, no. 5, 2005, pp. 2558-63.
Gunning RV, Dang HT, Kemp FC, et al. New resistance mechanism in Helicoverpa armigera threatens transgenic crops expressing Bacillus thuringiensis Cry1Ac toxin. Appl Environ Microbiol. 2005;71(5):2558-63.
Gunning, R. V., Dang, H. T., Kemp, F. C., Nicholson, I. C., & Moores, G. D. (2005). New resistance mechanism in Helicoverpa armigera threatens transgenic crops expressing Bacillus thuringiensis Cry1Ac toxin. Applied and Environmental Microbiology, 71(5), 2558-63.
Gunning RV, et al. New Resistance Mechanism in Helicoverpa Armigera Threatens Transgenic Crops Expressing Bacillus Thuringiensis Cry1Ac Toxin. Appl Environ Microbiol. 2005;71(5):2558-63. PubMed PMID: 15870346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New resistance mechanism in Helicoverpa armigera threatens transgenic crops expressing Bacillus thuringiensis Cry1Ac toxin. AU - Gunning,Robin V, AU - Dang,Ho T, AU - Kemp,Fred C, AU - Nicholson,Ian C, AU - Moores,Graham D, PY - 2005/5/5/pubmed PY - 2005/6/16/medline PY - 2005/5/5/entrez SP - 2558 EP - 63 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 71 IS - 5 N2 - In Australia, the cotton bollworm, Helicoverpa armigera, has a long history of resistance to conventional insecticides. Transgenic cotton (expressing the Bacillus thuringiensis toxin Cry1Ac) has been grown for H. armigera control since 1996. It is demonstrated here that a population of Australian H. armigera has developed resistance to Cry1Ac toxin (275-fold). Some 70% of resistant H. armigera larvae were able to survive on Cry1Ac transgenic cotton (Ingard) The resistance phenotype is inherited as an autosomal semidominant trait. Resistance was associated with elevated esterase levels, which cosegregated with resistance. In vitro studies employing surface plasmon resonance technology and other biochemical techniques demonstrated that resistant strain esterase could bind to Cry1Ac protoxin and activated toxin. In vivo studies showed that Cry1Ac-resistant larvae fed Cy1Ac transgenic cotton or Cry1Ac-treated artificial diet had lower esterase activity than non-Cry1Ac-fed larvae. A resistance mechanism in which esterase sequesters Cry1Ac is proposed. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/15870346/New_resistance_mechanism_in_Helicoverpa_armigera_threatens_transgenic_crops_expressing_Bacillus_thuringiensis_Cry1Ac_toxin_ DB - PRIME DP - Unbound Medicine ER -