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Detection of virulence factors in a corneal isolate of Klebsiella pneumoniae.
Ophthalmology. 2005 May; 112(5):883-7.O

Abstract

OBJECTIVE

To report on the microbiological findings of a Klebsiella pneumoniae strain isolated from a patient with keratitis.

DESIGN

Interventional case report.

INTERVENTION AND TESTING

Conjunctival swabs and corneal scrapings from the right eye were inoculated for culture. The isolate was analyzed for the presence of the mucoid phenotype and the ability to form biofilm. We also investigated whether the formation of biofilm by the corneal Klebsiella isolate is affected by N-acetylcysteine.

MAIN OUTCOME MEASURES

Culture results and biofilm production were analyzed.

RESULTS

K. pneumoniae was grown from the conjunctiva and cornea. The isolate showed the mucoid phenotype and strong biofilm production. N-acetylcysteine had an inhibitory effect on both biofilm formation and preformed biofilm.

CONCLUSIONS

K. pneumoniae can cause severe keratitis. The presence of virulence factors, such as the mucoid phenotype and the ability to form biofilm, may be important in determining corneal infection. N-acetylcysteine is a potential candidate for use as an inhibitor of Klebsiella biofilm formation.

Authors+Show Affiliations

Institute of Ophthalmology, University of Sassari, Sassari, Italy. apinna@uniss.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

15878071

Citation

Pinna, Antonio, et al. "Detection of Virulence Factors in a Corneal Isolate of Klebsiella Pneumoniae." Ophthalmology, vol. 112, no. 5, 2005, pp. 883-7.
Pinna A, Sechi LA, Zanetti S, et al. Detection of virulence factors in a corneal isolate of Klebsiella pneumoniae. Ophthalmology. 2005;112(5):883-7.
Pinna, A., Sechi, L. A., Zanetti, S., & Carta, F. (2005). Detection of virulence factors in a corneal isolate of Klebsiella pneumoniae. Ophthalmology, 112(5), 883-7.
Pinna A, et al. Detection of Virulence Factors in a Corneal Isolate of Klebsiella Pneumoniae. Ophthalmology. 2005;112(5):883-7. PubMed PMID: 15878071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of virulence factors in a corneal isolate of Klebsiella pneumoniae. AU - Pinna,Antonio, AU - Sechi,Leonardo A, AU - Zanetti,Stefania, AU - Carta,Francesco, PY - 2004/09/22/received PY - 2004/11/28/accepted PY - 2005/5/10/pubmed PY - 2005/5/20/medline PY - 2005/5/10/entrez SP - 883 EP - 7 JF - Ophthalmology JO - Ophthalmology VL - 112 IS - 5 N2 - OBJECTIVE: To report on the microbiological findings of a Klebsiella pneumoniae strain isolated from a patient with keratitis. DESIGN: Interventional case report. INTERVENTION AND TESTING: Conjunctival swabs and corneal scrapings from the right eye were inoculated for culture. The isolate was analyzed for the presence of the mucoid phenotype and the ability to form biofilm. We also investigated whether the formation of biofilm by the corneal Klebsiella isolate is affected by N-acetylcysteine. MAIN OUTCOME MEASURES: Culture results and biofilm production were analyzed. RESULTS: K. pneumoniae was grown from the conjunctiva and cornea. The isolate showed the mucoid phenotype and strong biofilm production. N-acetylcysteine had an inhibitory effect on both biofilm formation and preformed biofilm. CONCLUSIONS: K. pneumoniae can cause severe keratitis. The presence of virulence factors, such as the mucoid phenotype and the ability to form biofilm, may be important in determining corneal infection. N-acetylcysteine is a potential candidate for use as an inhibitor of Klebsiella biofilm formation. SN - 1549-4713 UR - https://www.unboundmedicine.com/medline/citation/15878071/Detection_of_virulence_factors_in_a_corneal_isolate_of_Klebsiella_pneumoniae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-6420(04)01902-5 DB - PRIME DP - Unbound Medicine ER -