Tags

Type your tag names separated by a space and hit enter

Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity.
Life Sci. 2005 Jun 03; 77(3):345-58.LS

Abstract

Due to the increasing importance of nitrosative stress in pathology and the efficacy displayed by flavonoids in cancelling the effects of peroxynitrite, we decided to conduct a comparative study of three commonly used beverages with the highest polyphenol contents and proven antioxidant properties: mate (Ilex paraguariensis); green tea (Camelia sinensis) extracts and white and red wines of the main varietals. We directly evaluated and compared the extracts and wines as protein nitration inhibitors using 3-nitrotyrosine as a biomarker, we studied the extracts as protectors from OONO-induced cytotoxicity in two mammalian cell lines. Both green tea and mate extracts have a high polyphenol content, in the case of Ip, its higher concentration and higher free radical quenching activity on the DPPH assay may be mainly due to the sui generis extraction procedure. When BSA was incubated in the presence of SIN-1, a time and dose dependent nitration of the protein is clearly shown. Co-incubation of BSA with Ip, green tea or red wines led to a dose dependent inhibition of the effect. Ip displayed the highest inhibitory activity, followed by red wines and the green tea. Dilutions as low as 1/1500 produced more than 80% inhibition of albumin nitration. When we studied peroxynitrite-induced cytotoxicity in murine RAW 264.7 macrophages and 31EG4 mammary cells., we found a potent, dose-dependent protective effect that was Ilex paraguariensis > red wines > green tea. Taken together, our results indicate that when the herbal preparations studied here are prepared the way they are usually drunk, Ip displays the highest inhibition of protein nitration, and the highest promotion of cell survival, whereas green tea or red wines display significant but lesser effects at the same concentrations. Further studies aiming at isolation of the active principles and assessment of their bioavailability are warranted.

Authors+Show Affiliations

Glycation, Oxidation and Disease Laboratory, Division of Basic Sciences, Touro University-California, 1310 Johnson Lane, Mare Island Vallejo, CA 94592, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15878361

Citation

Bixby, Mindy, et al. "Ilex Paraguariensis Extracts Are Potent Inhibitors of Nitrosative Stress: a Comparative Study With Green Tea and Wines Using a Protein Nitration Model and Mammalian Cell Cytotoxicity." Life Sciences, vol. 77, no. 3, 2005, pp. 345-58.
Bixby M, Spieler L, Menini T, et al. Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity. Life Sci. 2005;77(3):345-58.
Bixby, M., Spieler, L., Menini, T., & Gugliucci, A. (2005). Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity. Life Sciences, 77(3), 345-58.
Bixby M, et al. Ilex Paraguariensis Extracts Are Potent Inhibitors of Nitrosative Stress: a Comparative Study With Green Tea and Wines Using a Protein Nitration Model and Mammalian Cell Cytotoxicity. Life Sci. 2005 Jun 3;77(3):345-58. PubMed PMID: 15878361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity. AU - Bixby,Mindy, AU - Spieler,Lauren, AU - Menini,Teresita, AU - Gugliucci,Alejandro, Y1 - 2005/02/09/ PY - 2004/08/19/received PY - 2004/11/11/accepted PY - 2005/5/10/pubmed PY - 2005/7/9/medline PY - 2005/5/10/entrez SP - 345 EP - 58 JF - Life sciences JO - Life Sci VL - 77 IS - 3 N2 - Due to the increasing importance of nitrosative stress in pathology and the efficacy displayed by flavonoids in cancelling the effects of peroxynitrite, we decided to conduct a comparative study of three commonly used beverages with the highest polyphenol contents and proven antioxidant properties: mate (Ilex paraguariensis); green tea (Camelia sinensis) extracts and white and red wines of the main varietals. We directly evaluated and compared the extracts and wines as protein nitration inhibitors using 3-nitrotyrosine as a biomarker, we studied the extracts as protectors from OONO-induced cytotoxicity in two mammalian cell lines. Both green tea and mate extracts have a high polyphenol content, in the case of Ip, its higher concentration and higher free radical quenching activity on the DPPH assay may be mainly due to the sui generis extraction procedure. When BSA was incubated in the presence of SIN-1, a time and dose dependent nitration of the protein is clearly shown. Co-incubation of BSA with Ip, green tea or red wines led to a dose dependent inhibition of the effect. Ip displayed the highest inhibitory activity, followed by red wines and the green tea. Dilutions as low as 1/1500 produced more than 80% inhibition of albumin nitration. When we studied peroxynitrite-induced cytotoxicity in murine RAW 264.7 macrophages and 31EG4 mammary cells., we found a potent, dose-dependent protective effect that was Ilex paraguariensis > red wines > green tea. Taken together, our results indicate that when the herbal preparations studied here are prepared the way they are usually drunk, Ip displays the highest inhibition of protein nitration, and the highest promotion of cell survival, whereas green tea or red wines display significant but lesser effects at the same concentrations. Further studies aiming at isolation of the active principles and assessment of their bioavailability are warranted. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15878361/Ilex_paraguariensis_extracts_are_potent_inhibitors_of_nitrosative_stress:_a_comparative_study_with_green_tea_and_wines_using_a_protein_nitration_model_and_mammalian_cell_cytotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(05)00150-5 DB - PRIME DP - Unbound Medicine ER -