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Vinyl adhesive tape also effective for direct microscopy diagnosis of chromomycosis, lobomycosis, and paracoccidioidomycosis.
Diagn Microbiol Infect Dis. 2005 May; 52(1):39-43.DM

Abstract

Among the existing techniques for direct microscopy diagnosis in mycology, vinyl adhesive tape (VAT) preparations have proved their usefulness for Malassezia spp., Candida spp., and dermatophyte superficial infections. The collection and identification of fungal agents can be performed easily by the VAT technique, as demonstrated in this work, for the setting of some deep-seated cutaneous mycoses, where infectious agents can be observed in the horny layer of the epidermis in transepidermal elimination events. Preliminary results obtained in a series of 19 cases (chromomycosis, 12; lobomycosis, 5; paracoccidioidomycosis, 2) yielded positive results in 17 (89.47%). Failures for proper microscopic diagnosis detection happened in just 2 cases, 1 for chromomycosis and 1 for paracoccidioidomycosis. These results, although encouraging, were obtained from a relatively small patient population sample. Thus, the authors recommend further tests using this direct microscopy technique in a larger number of patients to validate these preliminary results and to explore its sensitivity in cases of small lesions.

Authors+Show Affiliations

Dermatology Service, Centro de Ciências da Saúde, Universidade Federal do Pará, 66055-240 Belém, Pará, Brazil. mariomir@amazon.com.brNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15878441

Citation

Miranda, Mario F R., and Ademir J G. Silva. "Vinyl Adhesive Tape Also Effective for Direct Microscopy Diagnosis of Chromomycosis, Lobomycosis, and Paracoccidioidomycosis." Diagnostic Microbiology and Infectious Disease, vol. 52, no. 1, 2005, pp. 39-43.
Miranda MF, Silva AJ. Vinyl adhesive tape also effective for direct microscopy diagnosis of chromomycosis, lobomycosis, and paracoccidioidomycosis. Diagn Microbiol Infect Dis. 2005;52(1):39-43.
Miranda, M. F., & Silva, A. J. (2005). Vinyl adhesive tape also effective for direct microscopy diagnosis of chromomycosis, lobomycosis, and paracoccidioidomycosis. Diagnostic Microbiology and Infectious Disease, 52(1), 39-43.
Miranda MF, Silva AJ. Vinyl Adhesive Tape Also Effective for Direct Microscopy Diagnosis of Chromomycosis, Lobomycosis, and Paracoccidioidomycosis. Diagn Microbiol Infect Dis. 2005;52(1):39-43. PubMed PMID: 15878441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vinyl adhesive tape also effective for direct microscopy diagnosis of chromomycosis, lobomycosis, and paracoccidioidomycosis. AU - Miranda,Mario F R, AU - Silva,Ademir J G, PY - 2004/12/02/received PY - 2005/02/05/accepted PY - 2005/5/10/pubmed PY - 2005/7/27/medline PY - 2005/5/10/entrez SP - 39 EP - 43 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 52 IS - 1 N2 - Among the existing techniques for direct microscopy diagnosis in mycology, vinyl adhesive tape (VAT) preparations have proved their usefulness for Malassezia spp., Candida spp., and dermatophyte superficial infections. The collection and identification of fungal agents can be performed easily by the VAT technique, as demonstrated in this work, for the setting of some deep-seated cutaneous mycoses, where infectious agents can be observed in the horny layer of the epidermis in transepidermal elimination events. Preliminary results obtained in a series of 19 cases (chromomycosis, 12; lobomycosis, 5; paracoccidioidomycosis, 2) yielded positive results in 17 (89.47%). Failures for proper microscopic diagnosis detection happened in just 2 cases, 1 for chromomycosis and 1 for paracoccidioidomycosis. These results, although encouraging, were obtained from a relatively small patient population sample. Thus, the authors recommend further tests using this direct microscopy technique in a larger number of patients to validate these preliminary results and to explore its sensitivity in cases of small lesions. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/15878441/Vinyl_adhesive_tape_also_effective_for_direct_microscopy_diagnosis_of_chromomycosis_lobomycosis_and_paracoccidioidomycosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(05)00035-0 DB - PRIME DP - Unbound Medicine ER -