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Protocatechuic aldehyde suppresses TNF-alpha-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells.
Eur J Pharmacol. 2005 Apr 18; 513(1-2):1-8.EJ

Abstract

Adhesion molecules, which play a crucial role in the development of atherogenesis, are produced by endothelial cells following stimulation with various inflammatory cytokines. The current studies examined the effect of a potent water-soluble antioxidant, protocatechuic aldehyde (derived from the Chinese herb, Salvia miltiorrhiza), on the expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-alpha (TNF-alpha). Protocatechuic aldehyde appeared to specifically downregulate the TNF-alpha-induced cell surface expression of vascular adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1) on HUVECs as well as the release of soluble VCAM-1and ICAM-1 from HUVECs in a dose-response manner at pharmacologically relevant concentrations (0.15-1.35 mM). We also observed a dose-dependent lowering of mRNA expression of VCAM-1 and ICAM-1 in the presence of protocatechuic aldehyde. Furthermore, protocatechuic aldehyde (0.15, 0.45, and 1.35 mM) notably inhibited TNF-alpha-induced upregulation of U937 cell adhesion to HUVECs to 83.7%, 60.9%, and 40.8%, respectively. A gel shift assay further showed that protocatechuic aldehyde inhibited the TNF-alpha-activated NF-kappaB and AP-1 DNA binding activities in a dose-dependent manner. Collectively, these results indicate that protocatechuic aldehyde inhibits TNF-alpha-stimulated VCAM-1 and ICAM-1expression in HUVECs through a mechanism that involves NF-kappaB and AP-1.

Authors+Show Affiliations

Beijing Institute of Radiation Medicine, Haidian District, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15878704

Citation

Zhou, Zhe, et al. "Protocatechuic Aldehyde Suppresses TNF-alpha-induced ICAM-1 and VCAM-1 Expression in Human Umbilical Vein Endothelial Cells." European Journal of Pharmacology, vol. 513, no. 1-2, 2005, pp. 1-8.
Zhou Z, Liu Y, Miao AD, et al. Protocatechuic aldehyde suppresses TNF-alpha-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells. Eur J Pharmacol. 2005;513(1-2):1-8.
Zhou, Z., Liu, Y., Miao, A. D., & Wang, S. Q. (2005). Protocatechuic aldehyde suppresses TNF-alpha-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells. European Journal of Pharmacology, 513(1-2), 1-8.
Zhou Z, et al. Protocatechuic Aldehyde Suppresses TNF-alpha-induced ICAM-1 and VCAM-1 Expression in Human Umbilical Vein Endothelial Cells. Eur J Pharmacol. 2005 Apr 18;513(1-2):1-8. PubMed PMID: 15878704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protocatechuic aldehyde suppresses TNF-alpha-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells. AU - Zhou,Zhe, AU - Liu,Yong, AU - Miao,Ai-Dong, AU - Wang,Sheng-Qi, PY - 2004/11/29/received PY - 2005/01/26/revised PY - 2005/01/31/accepted PY - 2005/5/10/pubmed PY - 2005/8/12/medline PY - 2005/5/10/entrez SP - 1 EP - 8 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 513 IS - 1-2 N2 - Adhesion molecules, which play a crucial role in the development of atherogenesis, are produced by endothelial cells following stimulation with various inflammatory cytokines. The current studies examined the effect of a potent water-soluble antioxidant, protocatechuic aldehyde (derived from the Chinese herb, Salvia miltiorrhiza), on the expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-alpha (TNF-alpha). Protocatechuic aldehyde appeared to specifically downregulate the TNF-alpha-induced cell surface expression of vascular adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1) on HUVECs as well as the release of soluble VCAM-1and ICAM-1 from HUVECs in a dose-response manner at pharmacologically relevant concentrations (0.15-1.35 mM). We also observed a dose-dependent lowering of mRNA expression of VCAM-1 and ICAM-1 in the presence of protocatechuic aldehyde. Furthermore, protocatechuic aldehyde (0.15, 0.45, and 1.35 mM) notably inhibited TNF-alpha-induced upregulation of U937 cell adhesion to HUVECs to 83.7%, 60.9%, and 40.8%, respectively. A gel shift assay further showed that protocatechuic aldehyde inhibited the TNF-alpha-activated NF-kappaB and AP-1 DNA binding activities in a dose-dependent manner. Collectively, these results indicate that protocatechuic aldehyde inhibits TNF-alpha-stimulated VCAM-1 and ICAM-1expression in HUVECs through a mechanism that involves NF-kappaB and AP-1. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/15878704/Protocatechuic_aldehyde_suppresses_TNF_alpha_induced_ICAM_1_and_VCAM_1_expression_in_human_umbilical_vein_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(05)00157-3 DB - PRIME DP - Unbound Medicine ER -