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Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibits mitochondrial lipid peroxidation induced by Fe2+-citrate.
Eur J Pharmacol. 2005 Apr 18; 513(1-2):47-55.EJ

Abstract

Mangiferin, a naturally occurring glucosylxanthone, has been described as having antidiabetic, antiproliferative, immunomodulatory and antioxidant activities. In this study we report for the first time the iron-complexing ability of mangiferin as a primary mechanism for protection of rat liver mitochondria against Fe(2+)-citrate induced lipid peroxidation. Thiobarbituric acid reactive substances and antimycin A-insensitive oxygen consumption were used as quantitative measures of lipid peroxidation. Mangiferin at 10 microM induced near-full protection against 50 microM Fe(2+)-citrate-induced mitochondrial swelling and loss of mitochondrial transmembrane potential (DeltaPsi). The IC(50) value for mangiferin protection against Fe(2+)-citrate-induced mitochondrial thiobarbituric acid reactive substance formation (9.02+/-1.12 microM) was around 10 times lower than that for tert-butylhydroperoxide mitochondrial induction of thiobarbituric acid reactive substance formation. The xanthone derivative also inhibited the iron citrate induction of mitochondrial antimycin A-insensitive oxygen consumption, stimulated oxygen consumption due to Fe(2+) autoxidation and prevented Fe(3+) ascorbate reduction. Absorption spectra of mangiferin-Fe(2+)/Fe(3+) complexes also suggest the formation of a transient charge transfer complex between Fe(2+) and mangiferin, accelerating Fe(2+) oxidation and the formation of a more stable Fe(3+)-mangiferin complex unable to participate in Fenton-type reaction and lipid peroxidation propagation phase. In conclusion, these results show that in vitro antioxidant activity of mangiferin is related to its iron-chelating properties and not merely due to the scavenging activity of free radicals. These results are of pharmacological relevance since mangiferin and its naturally contained extracts could be potential candidates for chelation therapy in diseases related to abnormal intracellular iron distribution or iron overload.

Authors+Show Affiliations

Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP, Brasil. g031071@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15878708

Citation

Andreu, Gilberto Pardo, et al. "Iron Complexing Activity of Mangiferin, a Naturally Occurring Glucosylxanthone, Inhibits Mitochondrial Lipid Peroxidation Induced By Fe2+-citrate." European Journal of Pharmacology, vol. 513, no. 1-2, 2005, pp. 47-55.
Andreu GP, Delgado R, Velho JA, et al. Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibits mitochondrial lipid peroxidation induced by Fe2+-citrate. Eur J Pharmacol. 2005;513(1-2):47-55.
Andreu, G. P., Delgado, R., Velho, J. A., Curti, C., & Vercesi, A. E. (2005). Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibits mitochondrial lipid peroxidation induced by Fe2+-citrate. European Journal of Pharmacology, 513(1-2), 47-55.
Andreu GP, et al. Iron Complexing Activity of Mangiferin, a Naturally Occurring Glucosylxanthone, Inhibits Mitochondrial Lipid Peroxidation Induced By Fe2+-citrate. Eur J Pharmacol. 2005 Apr 18;513(1-2):47-55. PubMed PMID: 15878708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibits mitochondrial lipid peroxidation induced by Fe2+-citrate. AU - Andreu,Gilberto Pardo, AU - Delgado,René, AU - Velho,Jesus A, AU - Curti,Carlos, AU - Vercesi,Anibal E, Y1 - 2005/04/01/ PY - 2005/02/17/received PY - 2005/03/02/accepted PY - 2005/5/10/pubmed PY - 2005/8/12/medline PY - 2005/5/10/entrez SP - 47 EP - 55 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 513 IS - 1-2 N2 - Mangiferin, a naturally occurring glucosylxanthone, has been described as having antidiabetic, antiproliferative, immunomodulatory and antioxidant activities. In this study we report for the first time the iron-complexing ability of mangiferin as a primary mechanism for protection of rat liver mitochondria against Fe(2+)-citrate induced lipid peroxidation. Thiobarbituric acid reactive substances and antimycin A-insensitive oxygen consumption were used as quantitative measures of lipid peroxidation. Mangiferin at 10 microM induced near-full protection against 50 microM Fe(2+)-citrate-induced mitochondrial swelling and loss of mitochondrial transmembrane potential (DeltaPsi). The IC(50) value for mangiferin protection against Fe(2+)-citrate-induced mitochondrial thiobarbituric acid reactive substance formation (9.02+/-1.12 microM) was around 10 times lower than that for tert-butylhydroperoxide mitochondrial induction of thiobarbituric acid reactive substance formation. The xanthone derivative also inhibited the iron citrate induction of mitochondrial antimycin A-insensitive oxygen consumption, stimulated oxygen consumption due to Fe(2+) autoxidation and prevented Fe(3+) ascorbate reduction. Absorption spectra of mangiferin-Fe(2+)/Fe(3+) complexes also suggest the formation of a transient charge transfer complex between Fe(2+) and mangiferin, accelerating Fe(2+) oxidation and the formation of a more stable Fe(3+)-mangiferin complex unable to participate in Fenton-type reaction and lipid peroxidation propagation phase. In conclusion, these results show that in vitro antioxidant activity of mangiferin is related to its iron-chelating properties and not merely due to the scavenging activity of free radicals. These results are of pharmacological relevance since mangiferin and its naturally contained extracts could be potential candidates for chelation therapy in diseases related to abnormal intracellular iron distribution or iron overload. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/15878708/Iron_complexing_activity_of_mangiferin_a_naturally_occurring_glucosylxanthone_inhibits_mitochondrial_lipid_peroxidation_induced_by_Fe2+_citrate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(05)00253-0 DB - PRIME DP - Unbound Medicine ER -