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Cloning and functional characterization of dog transient receptor potential vanilloid receptor-1 (TRPV1).
Eur J Pharmacol. 2005 Apr 18; 513(1-2):57-66.EJ

Abstract

Transient receptor potential vanilloid receptor-1 (TRPV1) is a sensory neuron-specific cation channel capable of integrating various noxious chemical and physical stimuli. The dog orthologue of TRPV1 was cloned using cDNA from nodose ganglia and heterologously expressed in HEK293(OFF) cells. At the amino acid level, dTRPV1 displays 85-89% sequence identity to other TRPV1 orthologues. Molecular pharmacological characterization of HEK293(OFF) cells expressing TRPV1 was assessed using a fluorescence imaging plate reader (FLIPR)-based calcium imaging assay. Dog TRPV1 was activated by various known TRPV1 agonists in a concentration-dependent manner: Ag23 = resiniferatoxin > olvanil approximately arvanil > capsaicin > phorbol 12-phenylacetate 13-acetate 20-homovanillate (PPAHV) > N-oleoyldopamine (OLDA). In addition, select TRPV1 antagonists (capsazepine, I-resiniferatoxin and N-(-4-tertiarybutylphenyl)-4-(3-cholorpyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC)) were able to block the response of dTRPV1 to capsaicin. Furthermore, the dog TRPV1 lacked a conserved protein kinase A (PKA) phosphorylation site (117) found in other cloned orthologues, which may have physiological consequences on dog TRPV1 function. Taken together, these data constitute the first study of the cloning, expression and pharmacological characterization of dog TRPV1.

Authors+Show Affiliations

Neurobiology, Schering-Plough Research Institute, Kenilworth, NJ 07033-0539, USA. providence.t.phelps@spcorp.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15878709

Citation

Phelps, P Tara, et al. "Cloning and Functional Characterization of Dog Transient Receptor Potential Vanilloid Receptor-1 (TRPV1)." European Journal of Pharmacology, vol. 513, no. 1-2, 2005, pp. 57-66.
Phelps PT, Anthes JC, Correll CC. Cloning and functional characterization of dog transient receptor potential vanilloid receptor-1 (TRPV1). Eur J Pharmacol. 2005;513(1-2):57-66.
Phelps, P. T., Anthes, J. C., & Correll, C. C. (2005). Cloning and functional characterization of dog transient receptor potential vanilloid receptor-1 (TRPV1). European Journal of Pharmacology, 513(1-2), 57-66.
Phelps PT, Anthes JC, Correll CC. Cloning and Functional Characterization of Dog Transient Receptor Potential Vanilloid Receptor-1 (TRPV1). Eur J Pharmacol. 2005 Apr 18;513(1-2):57-66. PubMed PMID: 15878709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and functional characterization of dog transient receptor potential vanilloid receptor-1 (TRPV1). AU - Phelps,P Tara, AU - Anthes,John C, AU - Correll,Craig C, Y1 - 2005/04/13/ PY - 2004/10/21/received PY - 2005/02/23/revised PY - 2005/02/25/accepted PY - 2005/5/10/pubmed PY - 2005/8/12/medline PY - 2005/5/10/entrez SP - 57 EP - 66 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 513 IS - 1-2 N2 - Transient receptor potential vanilloid receptor-1 (TRPV1) is a sensory neuron-specific cation channel capable of integrating various noxious chemical and physical stimuli. The dog orthologue of TRPV1 was cloned using cDNA from nodose ganglia and heterologously expressed in HEK293(OFF) cells. At the amino acid level, dTRPV1 displays 85-89% sequence identity to other TRPV1 orthologues. Molecular pharmacological characterization of HEK293(OFF) cells expressing TRPV1 was assessed using a fluorescence imaging plate reader (FLIPR)-based calcium imaging assay. Dog TRPV1 was activated by various known TRPV1 agonists in a concentration-dependent manner: Ag23 = resiniferatoxin > olvanil approximately arvanil > capsaicin > phorbol 12-phenylacetate 13-acetate 20-homovanillate (PPAHV) > N-oleoyldopamine (OLDA). In addition, select TRPV1 antagonists (capsazepine, I-resiniferatoxin and N-(-4-tertiarybutylphenyl)-4-(3-cholorpyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC)) were able to block the response of dTRPV1 to capsaicin. Furthermore, the dog TRPV1 lacked a conserved protein kinase A (PKA) phosphorylation site (117) found in other cloned orthologues, which may have physiological consequences on dog TRPV1 function. Taken together, these data constitute the first study of the cloning, expression and pharmacological characterization of dog TRPV1. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/15878709/Cloning_and_functional_characterization_of_dog_transient_receptor_potential_vanilloid_receptor_1__TRPV1__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(05)00240-2 DB - PRIME DP - Unbound Medicine ER -