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The role of substance P and bradykinin in the cough reflex and bronchoconstriction in guinea-pigs.
Eur J Pharmacol. 2005 Apr 18; 513(1-2):125-33.EJ

Abstract

In this study we investigated the ability of aerosolized substance P to induce either cough or bronchoconstriction in guinea-pigs. We have also examined whether pre-treatment, by the inhaled route, of animals with a combination of the neutral endopeptidase inhibitor, phosphoramidon (10(-3) M), and the diaminopeptidase IV inhibitor, diprotin A (10(-3) M), enhances the airway response to substance P. Moreover, we also assessed whether aerosol pre-treatment of guinea-pigs with either substance P or bradykinin, at 10(-4) M, affects the citric acid-induced cough and/or bronchoconstriction. Challenge of guinea-pigs with substance P only at 10(-3) M resulted in significant bronchconstriction but only a weak and variable cough response (1.1+/-0.6; P>0.05). Pre-treatment of guinea-pigs with both phosphoramidon and diprotin A resulted in a small non-significant increase in the cough response (2.8+/-0.9 vs. 1.1+/-0.6; P>0.05) but significantly enhanced substance P-induced bronchoconstriction (P<0.05). Moreover, exposure of guinea-pigs to substance P (10(-4) M) prior to citric acid challenge (0.6 M) resulted in a significant (P<0.05) enhancement of the citric acid-induced bronchoconstriction but not the citric acid-induced cough (11.7+/-1.8 vs. 12.8+/-1.5; P>0.05). In contrast, exposure of guinea-pigs to bradykinin (10(-4) M) prior to the citric acid challenge resulted in a significant enhancement of the cough response (9.2+/-1.9 vs. 25.8+/-2.5; P<0.05) but not the bronchoconstriction (P>0.05). These data do not support a major peripheral role for substance P in the cough reflex, although bradykinin is able to sensitize the cough reflex. Furthermore, these data suggest that bronchoconstriction, induced by citric acid, is not responsible for the cough associated with this irritant.

Authors+Show Affiliations

Department of Applied Therapeutics, Faculty of Pharmacy, Kuwait University. ahmed.elhashim@hsc.edu.kwNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15878717

Citation

El-Hashim, Ahmed Z., and Sanaa A. Amine. "The Role of Substance P and Bradykinin in the Cough Reflex and Bronchoconstriction in Guinea-pigs." European Journal of Pharmacology, vol. 513, no. 1-2, 2005, pp. 125-33.
El-Hashim AZ, Amine SA. The role of substance P and bradykinin in the cough reflex and bronchoconstriction in guinea-pigs. Eur J Pharmacol. 2005;513(1-2):125-33.
El-Hashim, A. Z., & Amine, S. A. (2005). The role of substance P and bradykinin in the cough reflex and bronchoconstriction in guinea-pigs. European Journal of Pharmacology, 513(1-2), 125-33.
El-Hashim AZ, Amine SA. The Role of Substance P and Bradykinin in the Cough Reflex and Bronchoconstriction in Guinea-pigs. Eur J Pharmacol. 2005 Apr 18;513(1-2):125-33. PubMed PMID: 15878717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of substance P and bradykinin in the cough reflex and bronchoconstriction in guinea-pigs. AU - El-Hashim,Ahmed Z, AU - Amine,Sanaa A, Y1 - 2005/04/07/ PY - 2004/07/22/received PY - 2005/01/27/revised PY - 2005/02/01/accepted PY - 2005/5/10/pubmed PY - 2005/8/12/medline PY - 2005/5/10/entrez SP - 125 EP - 33 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 513 IS - 1-2 N2 - In this study we investigated the ability of aerosolized substance P to induce either cough or bronchoconstriction in guinea-pigs. We have also examined whether pre-treatment, by the inhaled route, of animals with a combination of the neutral endopeptidase inhibitor, phosphoramidon (10(-3) M), and the diaminopeptidase IV inhibitor, diprotin A (10(-3) M), enhances the airway response to substance P. Moreover, we also assessed whether aerosol pre-treatment of guinea-pigs with either substance P or bradykinin, at 10(-4) M, affects the citric acid-induced cough and/or bronchoconstriction. Challenge of guinea-pigs with substance P only at 10(-3) M resulted in significant bronchconstriction but only a weak and variable cough response (1.1+/-0.6; P>0.05). Pre-treatment of guinea-pigs with both phosphoramidon and diprotin A resulted in a small non-significant increase in the cough response (2.8+/-0.9 vs. 1.1+/-0.6; P>0.05) but significantly enhanced substance P-induced bronchoconstriction (P<0.05). Moreover, exposure of guinea-pigs to substance P (10(-4) M) prior to citric acid challenge (0.6 M) resulted in a significant (P<0.05) enhancement of the citric acid-induced bronchoconstriction but not the citric acid-induced cough (11.7+/-1.8 vs. 12.8+/-1.5; P>0.05). In contrast, exposure of guinea-pigs to bradykinin (10(-4) M) prior to the citric acid challenge resulted in a significant enhancement of the cough response (9.2+/-1.9 vs. 25.8+/-2.5; P<0.05) but not the bronchoconstriction (P>0.05). These data do not support a major peripheral role for substance P in the cough reflex, although bradykinin is able to sensitize the cough reflex. Furthermore, these data suggest that bronchoconstriction, induced by citric acid, is not responsible for the cough associated with this irritant. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/15878717/The_role_of_substance_P_and_bradykinin_in_the_cough_reflex_and_bronchoconstriction_in_guinea_pigs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(05)00138-X DB - PRIME DP - Unbound Medicine ER -