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A mixed self-assembled monolayer-based surface plasmon immunosensor for detection of E. coli O157:H7.
Biosens Bioelectron. 2006 Jan 15; 21(7):998-1006.BB

Abstract

The sensitivity and specificity of a polyethylene glycol terminated alkanethiol mixed self-assembled monolayers (SAM) on surface plasmon resonance (SPR) immunosensor to detect Escherichia coli O157:H7 is demonstrated. Purified monoclonal (Mabs) or polyclonal antibodies (PAbs) against E. coli O157:H7 were immobilized on an activated sensor chip and direct and sandwich assays were carried to detect E. coli O157:H7. Effect of Protein G based detection and effect of concentrations of primary and secondary antibodies in sandwich assay were investigated. The sensor surface was observed under an optical microscope at various stages of the detection process. The sensor could detect as low as 10(3)CFU/ml of E. coli O157:H7 in a sandwich assay, with high specificity against Salmonella Enteritidis. The detection limit using direct assay and Protein G were 10(6)CFU/ml and 10(4)CFU/ml, respectively. Results indicate that an alkanethiol SAM based SPR biosensor has the potential for rapid and specific detection of E. coli O157:H7, using a sandwich assay.

Authors+Show Affiliations

Subramanian A
Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Irudayaraj J
No affiliation info available
Ryan T
No affiliation info available

MeSH

Antigen-Antibody ComplexBiosensing TechniquesCoated Materials, BiocompatibleColony Count, MicrobialEquipment DesignEquipment Failure AnalysisEscherichia coli O157ImmunoassaySurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15878825

Citation

Subramanian, Anand, et al. "A Mixed Self-assembled Monolayer-based Surface Plasmon Immunosensor for Detection of E. Coli O157:H7." Biosensors & Bioelectronics, vol. 21, no. 7, 2006, pp. 998-1006.
Subramanian A, Irudayaraj J, Ryan T. A mixed self-assembled monolayer-based surface plasmon immunosensor for detection of E. coli O157:H7. Biosens Bioelectron. 2006;21(7):998-1006.
Subramanian, A., Irudayaraj, J., & Ryan, T. (2006). A mixed self-assembled monolayer-based surface plasmon immunosensor for detection of E. coli O157:H7. Biosensors & Bioelectronics, 21(7), 998-1006.
Subramanian A, Irudayaraj J, Ryan T. A Mixed Self-assembled Monolayer-based Surface Plasmon Immunosensor for Detection of E. Coli O157:H7. Biosens Bioelectron. 2006 Jan 15;21(7):998-1006. PubMed PMID: 15878825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mixed self-assembled monolayer-based surface plasmon immunosensor for detection of E. coli O157:H7. AU - Subramanian,Anand, AU - Irudayaraj,Joseph, AU - Ryan,Thomas, PY - 2005/5/10/pubmed PY - 2006/3/18/medline PY - 2005/5/10/entrez SP - 998 EP - 1006 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 7 N2 - The sensitivity and specificity of a polyethylene glycol terminated alkanethiol mixed self-assembled monolayers (SAM) on surface plasmon resonance (SPR) immunosensor to detect Escherichia coli O157:H7 is demonstrated. Purified monoclonal (Mabs) or polyclonal antibodies (PAbs) against E. coli O157:H7 were immobilized on an activated sensor chip and direct and sandwich assays were carried to detect E. coli O157:H7. Effect of Protein G based detection and effect of concentrations of primary and secondary antibodies in sandwich assay were investigated. The sensor surface was observed under an optical microscope at various stages of the detection process. The sensor could detect as low as 10(3)CFU/ml of E. coli O157:H7 in a sandwich assay, with high specificity against Salmonella Enteritidis. The detection limit using direct assay and Protein G were 10(6)CFU/ml and 10(4)CFU/ml, respectively. Results indicate that an alkanethiol SAM based SPR biosensor has the potential for rapid and specific detection of E. coli O157:H7, using a sandwich assay. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15878825/A_mixed_self_assembled_monolayer_based_surface_plasmon_immunosensor_for_detection_of_E__coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -