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Expression of interferon beta in synovial tissue from patients with rheumatoid arthritis: comparison with patients with osteoarthritis and reactive arthritis.
Ann Rheum Dis. 2005 Dec; 64(12):1780-2.AR

Abstract

BACKGROUND

IFNbeta may have immunomodulatory effects in rheumatoid arthritis (RA) and its increased production in RA synovium may be a reactive attempt to inhibit inflammation.

OBJECTIVE

To determine the expression of IFNbeta in the synovial tissue of patients with RA, osteoarthritis, and reactive arthritis.

METHODS

Synovial biopsy specimens were obtained by arthroscopy from patients with RA and disease controls for immunohistological analysis using a monoclonal antibody specific for IFNbeta. Bound antibody was detected by an immunoperoxidase method. Stained sections were evaluated by computer assisted image analysis. Double stainings were performed with antibodies to detect CD55 positive fibroblast-like synoviocytes (FLS), CD68 positive macrophages, and CD83 positive dendritic cells (DCs) co-expressing IFNbeta.

RESULTS

IFNbeta protein was abundantly expressed in the synovium of patients with RA. Digital image analysis showed a significant increase in the mean integrated optical density for IFNbeta expression in RA synovial tissue compared with disease controls. Specific up regulation of IFNbeta expression was also seen when the results were controlled for cell numbers. Phenotypic analysis showed that FLS, especially, but also macrophages and DCs may express IFNbeta in RA synovial tissue.

CONCLUSIONS

The increased expression of IFNbeta in RA synovium suggests activation of an immunomodulatory mechanism that could inhibit synovial inflammation.

Authors+Show Affiliations

Division of Clinical Immunology and Rheumatology F4-218, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15878901

Citation

van Holten, J, et al. "Expression of Interferon Beta in Synovial Tissue From Patients With Rheumatoid Arthritis: Comparison With Patients With Osteoarthritis and Reactive Arthritis." Annals of the Rheumatic Diseases, vol. 64, no. 12, 2005, pp. 1780-2.
van Holten J, Smeets TJ, Blankert P, et al. Expression of interferon beta in synovial tissue from patients with rheumatoid arthritis: comparison with patients with osteoarthritis and reactive arthritis. Ann Rheum Dis. 2005;64(12):1780-2.
van Holten, J., Smeets, T. J., Blankert, P., & Tak, P. P. (2005). Expression of interferon beta in synovial tissue from patients with rheumatoid arthritis: comparison with patients with osteoarthritis and reactive arthritis. Annals of the Rheumatic Diseases, 64(12), 1780-2.
van Holten J, et al. Expression of Interferon Beta in Synovial Tissue From Patients With Rheumatoid Arthritis: Comparison With Patients With Osteoarthritis and Reactive Arthritis. Ann Rheum Dis. 2005;64(12):1780-2. PubMed PMID: 15878901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of interferon beta in synovial tissue from patients with rheumatoid arthritis: comparison with patients with osteoarthritis and reactive arthritis. AU - van Holten,J, AU - Smeets,T J M, AU - Blankert,P, AU - Tak,P P, Y1 - 2005/05/05/ PY - 2005/5/10/pubmed PY - 2006/1/10/medline PY - 2005/5/10/entrez SP - 1780 EP - 2 JF - Annals of the rheumatic diseases JO - Ann Rheum Dis VL - 64 IS - 12 N2 - BACKGROUND: IFNbeta may have immunomodulatory effects in rheumatoid arthritis (RA) and its increased production in RA synovium may be a reactive attempt to inhibit inflammation. OBJECTIVE: To determine the expression of IFNbeta in the synovial tissue of patients with RA, osteoarthritis, and reactive arthritis. METHODS: Synovial biopsy specimens were obtained by arthroscopy from patients with RA and disease controls for immunohistological analysis using a monoclonal antibody specific for IFNbeta. Bound antibody was detected by an immunoperoxidase method. Stained sections were evaluated by computer assisted image analysis. Double stainings were performed with antibodies to detect CD55 positive fibroblast-like synoviocytes (FLS), CD68 positive macrophages, and CD83 positive dendritic cells (DCs) co-expressing IFNbeta. RESULTS: IFNbeta protein was abundantly expressed in the synovium of patients with RA. Digital image analysis showed a significant increase in the mean integrated optical density for IFNbeta expression in RA synovial tissue compared with disease controls. Specific up regulation of IFNbeta expression was also seen when the results were controlled for cell numbers. Phenotypic analysis showed that FLS, especially, but also macrophages and DCs may express IFNbeta in RA synovial tissue. CONCLUSIONS: The increased expression of IFNbeta in RA synovium suggests activation of an immunomodulatory mechanism that could inhibit synovial inflammation. SN - 0003-4967 UR - https://www.unboundmedicine.com/medline/citation/15878901/Expression_of_interferon_beta_in_synovial_tissue_from_patients_with_rheumatoid_arthritis:_comparison_with_patients_with_osteoarthritis_and_reactive_arthritis_ L2 - https://ard.bmj.com/lookup/pmidlookup?view=long&pmid=15878901 DB - PRIME DP - Unbound Medicine ER -