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Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine environment.
Environ Mol Mutagen. 2005 Jul; 46(1):64-70.EM

Abstract

To evaluate the use of native fish species for assessing genotoxic pollution in the marine environment, micronucleus (MN) analysis was performed in peripheral blood erythrocytes and gill cells of the grey mullet (Mugil cephalus) from three sampling stations off the southeastern Mediterranean coast of Turkey. The frequencies of blebbed, notched, and lobed nuclei and binucleated cells also were evaluated in peripheral erythrocytes. The sampling sites were chosen on the basis of pollution levels; Karaduvar harbor, contaminated by different types of industrial effluents, and Mersin harbor, mainly contaminated by aromatic hydrocarbons, were selected as polluted areas. Erdemli harbor, a relatively unpolluted site, was used as the control area. Sampling was carried out at four different seasons. The frequencies of both micronuclei and other nuclear abnormalities (NAs) in mullets captured from polluted areas were significantly higher than those in mullets from the reference area. In general, gill cells had considerably higher MN frequencies than did erythrocytes, and genotoxic responses were higher in summer than in winter. The results of this study indicate that the MN test in fish is a suitable biomarker for in situ monitoring of genotoxic pollution in the marine environment. As demonstrated in this study, NAs other than micronuclei are also useful indices of chemical exposure and toxic responses. Therefore, measuring both micronuclei and NAs may increase the sensitivity of the test system.

Authors+Show Affiliations

Faculty of Sciences and Letters, Department of Biology, Mersin University, 33342-Mersin, Turkey. tcavas@mersin.edu.trNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15880416

Citation

Cavaş, Tolga, and Serap Ergene-Gözükara. "Micronucleus Test in Fish Cells: a Bioassay for in Situ Monitoring of Genotoxic Pollution in the Marine Environment." Environmental and Molecular Mutagenesis, vol. 46, no. 1, 2005, pp. 64-70.
Cavaş T, Ergene-Gözükara S. Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine environment. Environ Mol Mutagen. 2005;46(1):64-70.
Cavaş, T., & Ergene-Gözükara, S. (2005). Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine environment. Environmental and Molecular Mutagenesis, 46(1), 64-70.
Cavaş T, Ergene-Gözükara S. Micronucleus Test in Fish Cells: a Bioassay for in Situ Monitoring of Genotoxic Pollution in the Marine Environment. Environ Mol Mutagen. 2005;46(1):64-70. PubMed PMID: 15880416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine environment. AU - Cavaş,Tolga, AU - Ergene-Gözükara,Serap, PY - 2005/5/10/pubmed PY - 2005/10/1/medline PY - 2005/5/10/entrez SP - 64 EP - 70 JF - Environmental and molecular mutagenesis JO - Environ Mol Mutagen VL - 46 IS - 1 N2 - To evaluate the use of native fish species for assessing genotoxic pollution in the marine environment, micronucleus (MN) analysis was performed in peripheral blood erythrocytes and gill cells of the grey mullet (Mugil cephalus) from three sampling stations off the southeastern Mediterranean coast of Turkey. The frequencies of blebbed, notched, and lobed nuclei and binucleated cells also were evaluated in peripheral erythrocytes. The sampling sites were chosen on the basis of pollution levels; Karaduvar harbor, contaminated by different types of industrial effluents, and Mersin harbor, mainly contaminated by aromatic hydrocarbons, were selected as polluted areas. Erdemli harbor, a relatively unpolluted site, was used as the control area. Sampling was carried out at four different seasons. The frequencies of both micronuclei and other nuclear abnormalities (NAs) in mullets captured from polluted areas were significantly higher than those in mullets from the reference area. In general, gill cells had considerably higher MN frequencies than did erythrocytes, and genotoxic responses were higher in summer than in winter. The results of this study indicate that the MN test in fish is a suitable biomarker for in situ monitoring of genotoxic pollution in the marine environment. As demonstrated in this study, NAs other than micronuclei are also useful indices of chemical exposure and toxic responses. Therefore, measuring both micronuclei and NAs may increase the sensitivity of the test system. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/15880416/Micronucleus_test_in_fish_cells:_a_bioassay_for_in_situ_monitoring_of_genotoxic_pollution_in_the_marine_environment_ L2 - https://doi.org/10.1002/em.20130 DB - PRIME DP - Unbound Medicine ER -