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Choice of dietary protein of vegetarians and omnivores is reflected in their hair protein 13C and 15N abundance.
Rapid Commun Mass Spectrom. 2005; 19(11):1392-400.RC

Abstract

Stable isotopic (15N, 13C) composition of tissues depends on isotopic pattern of food sources. We investigated whether the isotopic compositions of human hair protein and amino acids reflect the habitual dietary protein intake. Hair samples were analyzed from 100 omnivores (selected randomly out of the 1987-1988 German nutrition survey VERA), and from 15 ovo-lacto-vegetarians (OLV), and from 6 vegans recruited separately. Hair bulk and amino acid specific isotopic compositions were analyzed by isotope-ratio mass spectrometry (EA/IRMS and GC/C/IRMS, respectively) and the results were correlated with data of the 7 day dietary records. Hair bulk 15N and 13C abundances clearly reflect the particular eating habits. Vegans can be distinguished from OLV and both are significantly distinct from omnivores in both 15N and 13C abundances. 15N and 13C abundances rose with a higher proportion of animal to total protein intake (PAPI). Individual proportions of animal protein consumption (IPAP) were calculated using isotopic abundances and a linear regression model using animal protein consumption data of vegans (PAPI = 0) and omnivores (mean PAPI = 0.639). IPAP values positively correlated with the intake of protein, meat, meat products, and animal protein. Distinct patterns for hair amino acid specific 15N and 13C abundances were measured but with lower resolution between food preference groups compared with bulk values. In conclusion, hair 13C and 15N values both reflected the extent of animal protein consumption. Bulk isotopic abundance of hair can be tested for future use in the validation of dietary assessment methods.

Authors+Show Affiliations

German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. petzke@mail.dife.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15880664

Citation

Petzke, Klaus J., et al. "Choice of Dietary Protein of Vegetarians and Omnivores Is Reflected in Their Hair Protein 13C and 15N Abundance." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 11, 2005, pp. 1392-400.
Petzke KJ, Boeing H, Metges CC. Choice of dietary protein of vegetarians and omnivores is reflected in their hair protein 13C and 15N abundance. Rapid Commun Mass Spectrom. 2005;19(11):1392-400.
Petzke, K. J., Boeing, H., & Metges, C. C. (2005). Choice of dietary protein of vegetarians and omnivores is reflected in their hair protein 13C and 15N abundance. Rapid Communications in Mass Spectrometry : RCM, 19(11), 1392-400.
Petzke KJ, Boeing H, Metges CC. Choice of Dietary Protein of Vegetarians and Omnivores Is Reflected in Their Hair Protein 13C and 15N Abundance. Rapid Commun Mass Spectrom. 2005;19(11):1392-400. PubMed PMID: 15880664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Choice of dietary protein of vegetarians and omnivores is reflected in their hair protein 13C and 15N abundance. AU - Petzke,Klaus J, AU - Boeing,Heiner, AU - Metges,Cornelia C, PY - 2005/5/10/pubmed PY - 2005/7/23/medline PY - 2005/5/10/entrez SP - 1392 EP - 400 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 11 N2 - Stable isotopic (15N, 13C) composition of tissues depends on isotopic pattern of food sources. We investigated whether the isotopic compositions of human hair protein and amino acids reflect the habitual dietary protein intake. Hair samples were analyzed from 100 omnivores (selected randomly out of the 1987-1988 German nutrition survey VERA), and from 15 ovo-lacto-vegetarians (OLV), and from 6 vegans recruited separately. Hair bulk and amino acid specific isotopic compositions were analyzed by isotope-ratio mass spectrometry (EA/IRMS and GC/C/IRMS, respectively) and the results were correlated with data of the 7 day dietary records. Hair bulk 15N and 13C abundances clearly reflect the particular eating habits. Vegans can be distinguished from OLV and both are significantly distinct from omnivores in both 15N and 13C abundances. 15N and 13C abundances rose with a higher proportion of animal to total protein intake (PAPI). Individual proportions of animal protein consumption (IPAP) were calculated using isotopic abundances and a linear regression model using animal protein consumption data of vegans (PAPI = 0) and omnivores (mean PAPI = 0.639). IPAP values positively correlated with the intake of protein, meat, meat products, and animal protein. Distinct patterns for hair amino acid specific 15N and 13C abundances were measured but with lower resolution between food preference groups compared with bulk values. In conclusion, hair 13C and 15N values both reflected the extent of animal protein consumption. Bulk isotopic abundance of hair can be tested for future use in the validation of dietary assessment methods. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15880664/Choice_of_dietary_protein_of_vegetarians_and_omnivores_is_reflected_in_their_hair_protein_13C_and_15N_abundance_ L2 - https://doi.org/10.1002/rcm.1925 DB - PRIME DP - Unbound Medicine ER -