Tags

Type your tag names separated by a space and hit enter

Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis.
Rapid Commun Mass Spectrom. 2005; 19(11):1529-35.RC

Abstract

This paper describes a rapid gas chromatographic/mass spectrometric (GC/MS) screening method for the detection of drugs of abuse and/or their metabolites in urine. Synthetic stimulants, opiates, cocaine metabolites, cannabinoids--and specifically the acid metabolite of tetrahydrocannabinol (THC-COOH)--can be simultaneously extracted by a single liquid/liquid separation step, at alkaline pH, and assayed as trimethylsilyl derivatives by GC/MS in SIM (selected ion monitoring) mode. All the analytes show a good linearity (R2 > 0.99 for most of the considered substances) in the range 25-1000 ng/mL, with a good reproducibility of both the retention times (CV% <0.7) and the relative abundances of the characteristic diagnostic ions (CV% <13). The limit of detection (LOD) of the method is 25 ng/mL of target compound in human urine for most of the substances investigated, 3 ng/mL for THC-COOH, and 10 ng/mL for norbuprenorphine. Validation of the method allows its application to different fields of forensic analytical toxicology, including antidoping analysis.

Authors+Show Affiliations

Laboratorio Antidoping, Federazione Medico Sportiva Italiana, Largo Giulio Onesti 1, 00197 Rome RM, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15880665

Citation

Strano-Rossi, Sabina, et al. "Rapid Screening of Drugs of Abuse and Their Metabolites By Gas Chromatography/mass Spectrometry: Application to Urinalysis." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 11, 2005, pp. 1529-35.
Strano-Rossi S, Molaioni F, Rossi F, et al. Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis. Rapid Commun Mass Spectrom. 2005;19(11):1529-35.
Strano-Rossi, S., Molaioni, F., Rossi, F., & Botrè, F. (2005). Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis. Rapid Communications in Mass Spectrometry : RCM, 19(11), 1529-35.
Strano-Rossi S, et al. Rapid Screening of Drugs of Abuse and Their Metabolites By Gas Chromatography/mass Spectrometry: Application to Urinalysis. Rapid Commun Mass Spectrom. 2005;19(11):1529-35. PubMed PMID: 15880665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis. AU - Strano-Rossi,Sabina, AU - Molaioni,Francesco, AU - Rossi,Francesca, AU - Botrè,Francesco, PY - 2005/5/10/pubmed PY - 2005/7/23/medline PY - 2005/5/10/entrez SP - 1529 EP - 35 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 11 N2 - This paper describes a rapid gas chromatographic/mass spectrometric (GC/MS) screening method for the detection of drugs of abuse and/or their metabolites in urine. Synthetic stimulants, opiates, cocaine metabolites, cannabinoids--and specifically the acid metabolite of tetrahydrocannabinol (THC-COOH)--can be simultaneously extracted by a single liquid/liquid separation step, at alkaline pH, and assayed as trimethylsilyl derivatives by GC/MS in SIM (selected ion monitoring) mode. All the analytes show a good linearity (R2 > 0.99 for most of the considered substances) in the range 25-1000 ng/mL, with a good reproducibility of both the retention times (CV% <0.7) and the relative abundances of the characteristic diagnostic ions (CV% <13). The limit of detection (LOD) of the method is 25 ng/mL of target compound in human urine for most of the substances investigated, 3 ng/mL for THC-COOH, and 10 ng/mL for norbuprenorphine. Validation of the method allows its application to different fields of forensic analytical toxicology, including antidoping analysis. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15880665/Rapid_screening_of_drugs_of_abuse_and_their_metabolites_by_gas_chromatography/mass_spectrometry:_application_to_urinalysis_ DB - PRIME DP - Unbound Medicine ER -