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Simultaneous separation and determination of Tarabine PFS and Adriblastine using micellar electrokinetic chromatography and high performance liquid chromatography. Application to some biological fluids.
J Sep Sci. 2005 Apr; 28(6):534-42.JS

Abstract

The simultaneous determination of Tarabine PFS and Adriblastine by two independent techniques, viz. micellar electrokinetic chromatography (MEKC) and high performance liquid chromatography (HPLC), has been studied. For MEKC analysis, separations and identifications were accomplished using uncoated fused-silica capillaries and injections were performed in the hydrodynamic mode. The running buffer consisted of 0.05 M borate/phosphate pH 8.70, with 0.10 M SDS at an operating voltage of 15.0 kV and the temperature held at 25.0 degrees C. Under these conditions, the migration times of Tarabine PFS and Adriblastine were 2.70 and 6.40 min, respectively. Calibration curves were established for 0.010-0.300 microg/mL (r = 0.99) Tarabine PFS and 8.000-120.0 microg/mL (r = 0.99) Adriblastine. The limit of detection (LOD) was estimated and found to be 0.003 and 3.000 microg/mL of Tarabine PFS and Adriblastine, respectively. The limit of quantitation (LOQ) was found to be 0.009 and 8.000 microg/mL of Tarabine PFS and Adriblastine, respectively. For HPLC analysis, separations and determinations were performed on teicoplanin stationary phase with reversed mobile phase containing methanol:buffer pH 4.05 (20.0:80.0%, v/v) at 285 nm. Calibration curves were established for 3.000-90.00 microg/mL (r = 0.99) Tarabine PFS and for 10.00-120.0 microg/mL (r = 0.99) Adriblastine. LOD and LOQ were estimated and found to be 0.950 and 2.050 microg/mL of Tarabine PFS and 3.130 and 9.250 microg/mL of Adriblastine, respectively. Both MEKC and HPLC methods were applied for the simultaneous determination of analytes in urine samples. It was found that 8.00-10.0% (Tarabine PFS) and 13.0-15.0% (Adriblastine) of the injected dose was recovered in urine samples with 99.5-102% recovery.

Authors+Show Affiliations

Abd El-Hady D
Chemistry Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. deiaabdelhady@yahoo.com
Gotti R
No affiliation info available
Abo El-Maali N
No affiliation info available
Andrisano V
No affiliation info available

MeSH

Antibiotics, AntineoplasticAntimetabolites, AntineoplasticChromatography, High Pressure LiquidChromatography, Micellar Electrokinetic CapillaryCytarabineDoxorubicinHumansMolecular StructureNeoplasmsReproducibility of ResultsSensitivity and SpecificityUrine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15881083

Citation

Abd El-Hady, Deia, et al. "Simultaneous Separation and Determination of Tarabine PFS and Adriblastine Using Micellar Electrokinetic Chromatography and High Performance Liquid Chromatography. Application to some Biological Fluids." Journal of Separation Science, vol. 28, no. 6, 2005, pp. 534-42.
Abd El-Hady D, Gotti R, Abo El-Maali N, et al. Simultaneous separation and determination of Tarabine PFS and Adriblastine using micellar electrokinetic chromatography and high performance liquid chromatography. Application to some biological fluids. J Sep Sci. 2005;28(6):534-42.
Abd El-Hady, D., Gotti, R., Abo El-Maali, N., & Andrisano, V. (2005). Simultaneous separation and determination of Tarabine PFS and Adriblastine using micellar electrokinetic chromatography and high performance liquid chromatography. Application to some biological fluids. Journal of Separation Science, 28(6), 534-42.
Abd El-Hady D, et al. Simultaneous Separation and Determination of Tarabine PFS and Adriblastine Using Micellar Electrokinetic Chromatography and High Performance Liquid Chromatography. Application to some Biological Fluids. J Sep Sci. 2005;28(6):534-42. PubMed PMID: 15881083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous separation and determination of Tarabine PFS and Adriblastine using micellar electrokinetic chromatography and high performance liquid chromatography. Application to some biological fluids. AU - Abd El-Hady,Deia, AU - Gotti,Roberto, AU - Abo El-Maali,Nagwa, AU - Andrisano,Vincenza, PY - 2005/5/11/pubmed PY - 2005/9/16/medline PY - 2005/5/11/entrez SP - 534 EP - 42 JF - Journal of separation science JO - J Sep Sci VL - 28 IS - 6 N2 - The simultaneous determination of Tarabine PFS and Adriblastine by two independent techniques, viz. micellar electrokinetic chromatography (MEKC) and high performance liquid chromatography (HPLC), has been studied. For MEKC analysis, separations and identifications were accomplished using uncoated fused-silica capillaries and injections were performed in the hydrodynamic mode. The running buffer consisted of 0.05 M borate/phosphate pH 8.70, with 0.10 M SDS at an operating voltage of 15.0 kV and the temperature held at 25.0 degrees C. Under these conditions, the migration times of Tarabine PFS and Adriblastine were 2.70 and 6.40 min, respectively. Calibration curves were established for 0.010-0.300 microg/mL (r = 0.99) Tarabine PFS and 8.000-120.0 microg/mL (r = 0.99) Adriblastine. The limit of detection (LOD) was estimated and found to be 0.003 and 3.000 microg/mL of Tarabine PFS and Adriblastine, respectively. The limit of quantitation (LOQ) was found to be 0.009 and 8.000 microg/mL of Tarabine PFS and Adriblastine, respectively. For HPLC analysis, separations and determinations were performed on teicoplanin stationary phase with reversed mobile phase containing methanol:buffer pH 4.05 (20.0:80.0%, v/v) at 285 nm. Calibration curves were established for 3.000-90.00 microg/mL (r = 0.99) Tarabine PFS and for 10.00-120.0 microg/mL (r = 0.99) Adriblastine. LOD and LOQ were estimated and found to be 0.950 and 2.050 microg/mL of Tarabine PFS and 3.130 and 9.250 microg/mL of Adriblastine, respectively. Both MEKC and HPLC methods were applied for the simultaneous determination of analytes in urine samples. It was found that 8.00-10.0% (Tarabine PFS) and 13.0-15.0% (Adriblastine) of the injected dose was recovered in urine samples with 99.5-102% recovery. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/15881083/Simultaneous_separation_and_determination_of_Tarabine_PFS_and_Adriblastine_using_micellar_electrokinetic_chromatography_and_high_performance_liquid_chromatography__Application_to_some_biological_fluids_ DB - PRIME DP - Unbound Medicine ER -