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Spectrophotometric determination of captopril with DTNB reagent in pharmaceutical formulation.
Boll Chim Farm. 2004 Jul-Aug; 143(6):249-51.BC

Abstract

A simple and sensitive spectrophotometeric method has been developed for the determination of captopril in its dosage form. The method is based on the reaction of the drug with DTNB reagent in pH 8 to produce a yellow coloured species measurable at 412 nm. The absorbance-concentration plot is linear over the range 1-10 x 10(-5) M with correlation coefficient of 0.997. The molar absorptivity and minimum delectability were 13553 and 3.2 x 10(-7) respectively. The proposed method was applied successfully for determination of captopril in its tablets form. The mean quantity and recovery were found to be 25.01+/-2% and 100.04+/-2% (each tablet contain 25 mg captopril). Quantification of the same tablets was determined by a standard method such as HPLC. The mean quantity of each tablet was found to be 25.07+/-1.22% by using HPLC method. It was not statistically, significant difference between Ellman's and HPLC method. This proposed method can be successfully applied to the determination of captopril in water and its dosage form and can use instead HPLC.

Authors+Show Affiliations

Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15881803

Citation

Hosseinimehr, S J., et al. "Spectrophotometric Determination of Captopril With DTNB Reagent in Pharmaceutical Formulation." Bollettino Chimico Farmaceutico, vol. 143, no. 6, 2004, pp. 249-51.
Hosseinimehr SJ, Ebrahimi P, Hassani N, et al. Spectrophotometric determination of captopril with DTNB reagent in pharmaceutical formulation. Boll Chim Farm. 2004;143(6):249-51.
Hosseinimehr, S. J., Ebrahimi, P., Hassani, N., Mirzabeigi, P., & Amini, M. (2004). Spectrophotometric determination of captopril with DTNB reagent in pharmaceutical formulation. Bollettino Chimico Farmaceutico, 143(6), 249-51.
Hosseinimehr SJ, et al. Spectrophotometric Determination of Captopril With DTNB Reagent in Pharmaceutical Formulation. Boll Chim Farm. 2004 Jul-Aug;143(6):249-51. PubMed PMID: 15881803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spectrophotometric determination of captopril with DTNB reagent in pharmaceutical formulation. AU - Hosseinimehr,S J, AU - Ebrahimi,P, AU - Hassani,N, AU - Mirzabeigi,P, AU - Amini,M, PY - 2005/5/11/pubmed PY - 2005/6/29/medline PY - 2005/5/11/entrez SP - 249 EP - 51 JF - Bollettino chimico farmaceutico JO - Boll Chim Farm VL - 143 IS - 6 N2 - A simple and sensitive spectrophotometeric method has been developed for the determination of captopril in its dosage form. The method is based on the reaction of the drug with DTNB reagent in pH 8 to produce a yellow coloured species measurable at 412 nm. The absorbance-concentration plot is linear over the range 1-10 x 10(-5) M with correlation coefficient of 0.997. The molar absorptivity and minimum delectability were 13553 and 3.2 x 10(-7) respectively. The proposed method was applied successfully for determination of captopril in its tablets form. The mean quantity and recovery were found to be 25.01+/-2% and 100.04+/-2% (each tablet contain 25 mg captopril). Quantification of the same tablets was determined by a standard method such as HPLC. The mean quantity of each tablet was found to be 25.07+/-1.22% by using HPLC method. It was not statistically, significant difference between Ellman's and HPLC method. This proposed method can be successfully applied to the determination of captopril in water and its dosage form and can use instead HPLC. SN - 0006-6648 UR - https://www.unboundmedicine.com/medline/citation/15881803/Spectrophotometric_determination_of_captopril_with_DTNB_reagent_in_pharmaceutical_formulation_ DB - PRIME DP - Unbound Medicine ER -