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Oxidative stress and genotoxic responses to resin acids in Mediterranean mussels.
Ecotoxicol Environ Saf. 2005 Jun; 61(2):221-9.EE

Abstract

This study represents the first attempt to investigate the genotoxic effects and oxidative stress of resin acids in Mediterranean mussels (Mytilus galloprovincialis Lmk). Mussels were exposed to 2.7 microM abietic acid (AA) and dehydroabietic acid (DHAA) for 6, 12, 18, and 24h. Gill and hepatopancreas conjugation activity, antioxidant defense system, lipid peroxidation (LPO), and DNA damage were determined as reduced glutathione (GSH), glutathione S-transferase (GST) activity, glutathione peroxidase (GPx) activity, catalase (CAT) activity, LPO, and DNA strand breaks. AA caused significant GST inhibition in mussel gills at 12, 18, and 24h. Activity of the antioxidant enzymes, namely, GPx and CAT, was inhibited at 24 and 18 h, respectively, in mussel gills. A significant increase in gill LPO was observed at 24h. The DNA integrity of mussel hepatopancreas significantly decreased after 12 and 24 h exposure to AA. A significant increase in LPO was observed after 6h exposure to DHAA, in either mussel gills or hepatopancreas. DNA integrity was significantly decreased in mussel hepatopancreas after 12 and 24 h exposure to DHAA. AA induced oxidative damage and genotoxicity in mussels, because it promoted increases in LPO in gills and DNA strand breaks in hepatopancreas. DHAA promoted oxidative damage and genotoxicity in mussels, as significant increases were observed in LPO in gills and hepatopancreas and in DNA strand breaks in hepatopancreas.

Authors+Show Affiliations

Department of Biology, Animal Physiology/Ecotoxicology Sector, University of Aveiro, 3810-193 Aveiro, Portugal. gravaoc@bio.ua.ptNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15883093

Citation

Gravato, C, et al. "Oxidative Stress and Genotoxic Responses to Resin Acids in Mediterranean Mussels." Ecotoxicology and Environmental Safety, vol. 61, no. 2, 2005, pp. 221-9.
Gravato C, Oliveira M, Santos MA. Oxidative stress and genotoxic responses to resin acids in Mediterranean mussels. Ecotoxicol Environ Saf. 2005;61(2):221-9.
Gravato, C., Oliveira, M., & Santos, M. A. (2005). Oxidative stress and genotoxic responses to resin acids in Mediterranean mussels. Ecotoxicology and Environmental Safety, 61(2), 221-9.
Gravato C, Oliveira M, Santos MA. Oxidative Stress and Genotoxic Responses to Resin Acids in Mediterranean Mussels. Ecotoxicol Environ Saf. 2005;61(2):221-9. PubMed PMID: 15883093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress and genotoxic responses to resin acids in Mediterranean mussels. AU - Gravato,C, AU - Oliveira,M, AU - Santos,M A, PY - 2003/12/29/received PY - 2004/11/02/revised PY - 2004/12/27/accepted PY - 2005/5/11/pubmed PY - 2005/9/15/medline PY - 2005/5/11/entrez SP - 221 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 61 IS - 2 N2 - This study represents the first attempt to investigate the genotoxic effects and oxidative stress of resin acids in Mediterranean mussels (Mytilus galloprovincialis Lmk). Mussels were exposed to 2.7 microM abietic acid (AA) and dehydroabietic acid (DHAA) for 6, 12, 18, and 24h. Gill and hepatopancreas conjugation activity, antioxidant defense system, lipid peroxidation (LPO), and DNA damage were determined as reduced glutathione (GSH), glutathione S-transferase (GST) activity, glutathione peroxidase (GPx) activity, catalase (CAT) activity, LPO, and DNA strand breaks. AA caused significant GST inhibition in mussel gills at 12, 18, and 24h. Activity of the antioxidant enzymes, namely, GPx and CAT, was inhibited at 24 and 18 h, respectively, in mussel gills. A significant increase in gill LPO was observed at 24h. The DNA integrity of mussel hepatopancreas significantly decreased after 12 and 24 h exposure to AA. A significant increase in LPO was observed after 6h exposure to DHAA, in either mussel gills or hepatopancreas. DNA integrity was significantly decreased in mussel hepatopancreas after 12 and 24 h exposure to DHAA. AA induced oxidative damage and genotoxicity in mussels, because it promoted increases in LPO in gills and DNA strand breaks in hepatopancreas. DHAA promoted oxidative damage and genotoxicity in mussels, as significant increases were observed in LPO in gills and hepatopancreas and in DNA strand breaks in hepatopancreas. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/15883093/Oxidative_stress_and_genotoxic_responses_to_resin_acids_in_Mediterranean_mussels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(04)00261-1 DB - PRIME DP - Unbound Medicine ER -