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CD137 induces adhesion and cytokine production in human monocytic THP-1 cells.
Exp Mol Med. 2005 Apr 30; 37(2):78-85.EM

Abstract

CD137, which is expressed on activated T cells, plays a critical role in inflammatory responses. However, the exact role that CD137 plays in monocytes is not fully known. Here we studied the expression and function of CD137 in human monocytic THP-1 cells, which we found constitutively expresses CD137 at the mRNA and protein level. Cross-linking of CD137 increased the secretion of IL-8 and TNF-alpha, promoted the expression of CD54 and CD11b, and increased adhesion to extracellular matrix (ECM) proteins. In particular CD137-induced adhesion of THP-1 cells was inhibited by an inhibitor of mitogen-activated protein kinase kinase (MEK), but not by a p38 kinase inhibitor. Taken together, these results show that the adhesion and cytokine production of THP-1 cells induced by CD137 occur via activation of MEK, which results in the activation of ERK-1/2 signaling pathways. Therefore, this study suggests that CD137 induces an activating and migrating signal during inflammatory processes.

Authors+Show Affiliations

National Genome Research Institute, National Institute of Health, KCDC, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15886520

Citation

Choi, Jung-Won, et al. "CD137 Induces Adhesion and Cytokine Production in Human Monocytic THP-1 Cells." Experimental & Molecular Medicine, vol. 37, no. 2, 2005, pp. 78-85.
Choi JW, Lee HW, Roh GS, et al. CD137 induces adhesion and cytokine production in human monocytic THP-1 cells. Exp Mol Med. 2005;37(2):78-85.
Choi, J. W., Lee, H. W., Roh, G. S., Kim, H. H., & Kwack, K. (2005). CD137 induces adhesion and cytokine production in human monocytic THP-1 cells. Experimental & Molecular Medicine, 37(2), 78-85.
Choi JW, et al. CD137 Induces Adhesion and Cytokine Production in Human Monocytic THP-1 Cells. Exp Mol Med. 2005 Apr 30;37(2):78-85. PubMed PMID: 15886520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD137 induces adhesion and cytokine production in human monocytic THP-1 cells. AU - Choi,Jung-Won, AU - Lee,Hyeon-Woo, AU - Roh,Gu-Seob, AU - Kim,Hong-Hee, AU - Kwack,Kyubum, PY - 2005/5/12/pubmed PY - 2005/8/27/medline PY - 2005/5/12/entrez SP - 78 EP - 85 JF - Experimental & molecular medicine JO - Exp Mol Med VL - 37 IS - 2 N2 - CD137, which is expressed on activated T cells, plays a critical role in inflammatory responses. However, the exact role that CD137 plays in monocytes is not fully known. Here we studied the expression and function of CD137 in human monocytic THP-1 cells, which we found constitutively expresses CD137 at the mRNA and protein level. Cross-linking of CD137 increased the secretion of IL-8 and TNF-alpha, promoted the expression of CD54 and CD11b, and increased adhesion to extracellular matrix (ECM) proteins. In particular CD137-induced adhesion of THP-1 cells was inhibited by an inhibitor of mitogen-activated protein kinase kinase (MEK), but not by a p38 kinase inhibitor. Taken together, these results show that the adhesion and cytokine production of THP-1 cells induced by CD137 occur via activation of MEK, which results in the activation of ERK-1/2 signaling pathways. Therefore, this study suggests that CD137 induces an activating and migrating signal during inflammatory processes. SN - 1226-3613 UR - https://www.unboundmedicine.com/medline/citation/15886520/CD137_induces_adhesion_and_cytokine_production_in_human_monocytic_THP_1_cells_ L2 - https://antibodies.cancer.gov/detail/CPTC-TNFRSF9-1 DB - PRIME DP - Unbound Medicine ER -