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Laser-induced dissociation/high-energy collision-induced dissociation fragmentation using MALDI-TOF/TOF-MS instrumentation for the analysis of neutral and acidic oligosaccharides.
Anal Chem. 2005 May 15; 77(10):3274-83.AC

Abstract

Producing detailed mass spectrometric fragmentation data of native oligosaccharides for the purpose of basic structure elucidation has become a readily accessible tool since the availability of enhanced technical equipment. In this report, high-energy collision-induced dissociation (heCID) in combination with MALDI-TOF/TOF technology for analysis of native neutral and acidic oligosaccharides is described. By providing complementary data, heCID-MALDI-TOF/TOF adds a variety of valuable cross-ring fragmentation information to the information of glycosidic fragmentation obtained preferably by laser-induced dissociation (LID). We examined parameters influencing fragmentation behavior of both-acidic and neutral-compounds. Results show a dependency of the fragmentation pattern for the employed matrix as well as the laser intensity provided for the ionization of the analytes and the complexity of the analytes. Due to instrument-specific settings, protonated glycosidic ion series within spectra of sodiated compounds could also be identified. Furthermore, acquired spectra could be readily used to identify compounds by comparison to existing glycan databases such as GlycoSuiteDB and GlycosciencesDB. The results show a better scoring of heCID data sets in comparison to LID-derived data. heCID-MALDI-TOF/TOF analysis in combination with database search algorithms is demonstrated to be suitable for an initial identification/classification of carbohydrates.

Authors+Show Affiliations

Rudolf-Virchow Center for Experimental Biomedicine, Versbacher Strasse 9, 97078 Wuerzburg, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15889919

Citation

Lewandrowski, Urs, et al. "Laser-induced Dissociation/high-energy Collision-induced Dissociation Fragmentation Using MALDI-TOF/TOF-MS Instrumentation for the Analysis of Neutral and Acidic Oligosaccharides." Analytical Chemistry, vol. 77, no. 10, 2005, pp. 3274-83.
Lewandrowski U, Resemann A, Sickmann A. Laser-induced dissociation/high-energy collision-induced dissociation fragmentation using MALDI-TOF/TOF-MS instrumentation for the analysis of neutral and acidic oligosaccharides. Anal Chem. 2005;77(10):3274-83.
Lewandrowski, U., Resemann, A., & Sickmann, A. (2005). Laser-induced dissociation/high-energy collision-induced dissociation fragmentation using MALDI-TOF/TOF-MS instrumentation for the analysis of neutral and acidic oligosaccharides. Analytical Chemistry, 77(10), 3274-83.
Lewandrowski U, Resemann A, Sickmann A. Laser-induced Dissociation/high-energy Collision-induced Dissociation Fragmentation Using MALDI-TOF/TOF-MS Instrumentation for the Analysis of Neutral and Acidic Oligosaccharides. Anal Chem. 2005 May 15;77(10):3274-83. PubMed PMID: 15889919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laser-induced dissociation/high-energy collision-induced dissociation fragmentation using MALDI-TOF/TOF-MS instrumentation for the analysis of neutral and acidic oligosaccharides. AU - Lewandrowski,Urs, AU - Resemann,Anja, AU - Sickmann,Albert, PY - 2005/5/14/pubmed PY - 2007/3/14/medline PY - 2005/5/14/entrez SP - 3274 EP - 83 JF - Analytical chemistry JO - Anal Chem VL - 77 IS - 10 N2 - Producing detailed mass spectrometric fragmentation data of native oligosaccharides for the purpose of basic structure elucidation has become a readily accessible tool since the availability of enhanced technical equipment. In this report, high-energy collision-induced dissociation (heCID) in combination with MALDI-TOF/TOF technology for analysis of native neutral and acidic oligosaccharides is described. By providing complementary data, heCID-MALDI-TOF/TOF adds a variety of valuable cross-ring fragmentation information to the information of glycosidic fragmentation obtained preferably by laser-induced dissociation (LID). We examined parameters influencing fragmentation behavior of both-acidic and neutral-compounds. Results show a dependency of the fragmentation pattern for the employed matrix as well as the laser intensity provided for the ionization of the analytes and the complexity of the analytes. Due to instrument-specific settings, protonated glycosidic ion series within spectra of sodiated compounds could also be identified. Furthermore, acquired spectra could be readily used to identify compounds by comparison to existing glycan databases such as GlycoSuiteDB and GlycosciencesDB. The results show a better scoring of heCID data sets in comparison to LID-derived data. heCID-MALDI-TOF/TOF analysis in combination with database search algorithms is demonstrated to be suitable for an initial identification/classification of carbohydrates. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15889919/Laser_induced_dissociation/high_energy_collision_induced_dissociation_fragmentation_using_MALDI_TOF/TOF_MS_instrumentation_for_the_analysis_of_neutral_and_acidic_oligosaccharides_ L2 - https://doi.org/10.1021/ac048399n DB - PRIME DP - Unbound Medicine ER -