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Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction.
Am J Trop Med Hyg. 2005 May; 72(5):640-1.AJ

Abstract

Orientia tsutsugamushi infection causes scrub typhus, a common zoonosis of rural Asia. Orientia tsutsugamushi was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals (P = 0.003, Fisher's two-tailed exact test). As few as 1,076 O. tsutsugamushi copies/microL were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection.

Authors+Show Affiliations

Singhsilarak T
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmtss@mahidol.ac.th
Leowattana W
No affiliation info available
Looareesuwan S
No affiliation info available
Wongchotigul V
No affiliation info available
Jiang J
No affiliation info available
Richards AL
No affiliation info available
Watt G
No affiliation info available

MeSH

DNA, BacterialHumansOrientia tsutsugamushiPolymerase Chain ReactionScrub TyphusSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15891142

Citation

Singhsilarak, Tasawan, et al. "Short Report: Detection of Orientia Tsutsugamushi in Clinical Samples By Quantitative Real-time Polymerase Chain Reaction." The American Journal of Tropical Medicine and Hygiene, vol. 72, no. 5, 2005, pp. 640-1.
Singhsilarak T, Leowattana W, Looareesuwan S, et al. Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. Am J Trop Med Hyg. 2005;72(5):640-1.
Singhsilarak, T., Leowattana, W., Looareesuwan, S., Wongchotigul, V., Jiang, J., Richards, A. L., & Watt, G. (2005). Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. The American Journal of Tropical Medicine and Hygiene, 72(5), 640-1.
Singhsilarak T, et al. Short Report: Detection of Orientia Tsutsugamushi in Clinical Samples By Quantitative Real-time Polymerase Chain Reaction. Am J Trop Med Hyg. 2005;72(5):640-1. PubMed PMID: 15891142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. AU - Singhsilarak,Tasawan, AU - Leowattana,Wattana, AU - Looareesuwan,Sornchai, AU - Wongchotigul,Varee, AU - Jiang,Ju, AU - Richards,Allen L, AU - Watt,George, PY - 2005/5/14/pubmed PY - 2005/6/29/medline PY - 2005/5/14/entrez SP - 640 EP - 1 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 72 IS - 5 N2 - Orientia tsutsugamushi infection causes scrub typhus, a common zoonosis of rural Asia. Orientia tsutsugamushi was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals (P = 0.003, Fisher's two-tailed exact test). As few as 1,076 O. tsutsugamushi copies/microL were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/15891142/Short_report:_detection_of_Orientia_tsutsugamushi_in_clinical_samples_by_quantitative_real_time_polymerase_chain_reaction_ DB - PRIME DP - Unbound Medicine ER -