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Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock.
Environ Microbiol. 2005 Jun; 7(6):789-97.EM

Abstract

Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85 degrees C to 65 degrees C and 95 degrees C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95 degrees C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H+ transporting ATP synthase. A gene encoding an alpha subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65 degrees C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions.

Authors+Show Affiliations

Boonyaratanakornkit BB
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.
Simpson AJ
No affiliation info available
Whitehead TA
No affiliation info available
Fraser CM
No affiliation info available
El-Sayed NM
No affiliation info available
Clark DS
No affiliation info available

MeSH

Adaptation, PhysiologicalArchaeal ProteinsCold TemperatureGene Expression ProfilingGene Expression Regulation, ArchaealHeat-Shock ProteinsHot TemperatureMethanococcusTemperatureTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15892698

Citation

Boonyaratanakornkit, Boonchai B., et al. "Transcriptional Profiling of the Hyperthermophilic Methanarchaeon Methanococcus Jannaschii in Response to Lethal Heat and Non-lethal Cold Shock." Environmental Microbiology, vol. 7, no. 6, 2005, pp. 789-97.
Boonyaratanakornkit BB, Simpson AJ, Whitehead TA, et al. Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock. Environ Microbiol. 2005;7(6):789-97.
Boonyaratanakornkit, B. B., Simpson, A. J., Whitehead, T. A., Fraser, C. M., El-Sayed, N. M., & Clark, D. S. (2005). Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock. Environmental Microbiology, 7(6), 789-97.
Boonyaratanakornkit BB, et al. Transcriptional Profiling of the Hyperthermophilic Methanarchaeon Methanococcus Jannaschii in Response to Lethal Heat and Non-lethal Cold Shock. Environ Microbiol. 2005;7(6):789-97. PubMed PMID: 15892698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock. AU - Boonyaratanakornkit,Boonchai B, AU - Simpson,Anjana J, AU - Whitehead,Timothy A, AU - Fraser,Claire M, AU - El-Sayed,Najib M A, AU - Clark,Douglas S, PY - 2005/5/17/pubmed PY - 2005/8/10/medline PY - 2005/5/17/entrez SP - 789 EP - 97 JF - Environmental microbiology JO - Environ Microbiol VL - 7 IS - 6 N2 - Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85 degrees C to 65 degrees C and 95 degrees C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95 degrees C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H+ transporting ATP synthase. A gene encoding an alpha subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65 degrees C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions. SN - 1462-2912 UR - https://www.unboundmedicine.com/medline/citation/15892698/Transcriptional_profiling_of_the_hyperthermophilic_methanarchaeon_Methanococcus_jannaschii_in_response_to_lethal_heat_and_non_lethal_cold_shock_ DB - PRIME DP - Unbound Medicine ER -