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Detection of specific antibodies to Neospora caninum and Toxoplasma gondii in naturally infected alpacas (Lama pacos), llamas (Lama glama) and vicunas (Lama vicugna) from Peru and Germany.
Vet Parasitol. 2005 Jun 10; 130(1-2):81-7.VP

Abstract

Sera of an experimentally Neospora caninum infected llama and a non-infected control llama were used to establish an immunoblot, an ELISA and an IFAT to detect antibodies against N. caninum tachyzoites. Subsequently, serum samples collected from a total of 871 South American Camelids (SAC: Lama glama, Lama pacos, Lama vicugna) of two farms in Peru and from 32 SAC of a farm in central Germany were examined for antibodies against N. caninum and Toxoplasma gondii. Based on the recognition of specific bands in the immunoblot, sera of SAC from Peru were differentiated into N. caninum-positive (n = 18) and T. gondii-positive (n = 30) samples and into samples negative or inconclusive for both parasites. Using the immunoblot results as the reference, a modified version of the p38-ELISA and the IFAT were evaluated for detecting N. caninum antibodies in SAC sera. Applying a cut-off as determined by two graph-receiver operating characteristic analysis both, the ELISA and the IFAT, exhibited a sensitivity and specificity of about 95% in the SAC sera from Peru. Serological testing confirmed that SAC may become infected with N. caninum under field conditions in Peru. In addition to alpacas and llamas also 114 wild living vicunas had been examined for antibodies against N. caninum. However, only the alpacas and llamas but no vicunas were found N. caninum-positive. In contrast, T. gondii-seropositive animals were detected in all three SAC species. The lack of N. caninum-seropositive vicunas indicates that in the study area in Peru wild canids might not serve as definitive hosts of N. caninum while for T. gondii a life cycle including wild felids is likely. On the German farm no N. caninum- but only T. gondii-seropositive SAC (n = 14) were detected. The seroprevalence of T. gondii infection was significantly higher in adult SAC (alpacas in Peru, llamas in Germany) than in crias (i.e. < 12 months old foals) indicating that the predominant route of infection is post natal. Since the present study was restricted to a few farms, the seroprevalences determined are not representative. However, our results confirm natural infections with N. caninum and T. gondii in SAC. Whether these infections are linked to any disease, e.g. reproductive losses, has to be clarified in further studies.

Authors+Show Affiliations

Institute of Parasitology, Justus Liebig University Giessen, Rudolf-Buchheim-Strasse 2, D-35392 Giessen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15893073

Citation

Wolf, D, et al. "Detection of Specific Antibodies to Neospora Caninum and Toxoplasma Gondii in Naturally Infected Alpacas (Lama Pacos), Llamas (Lama Glama) and Vicunas (Lama Vicugna) From Peru and Germany." Veterinary Parasitology, vol. 130, no. 1-2, 2005, pp. 81-7.
Wolf D, Schares G, Cardenas O, et al. Detection of specific antibodies to Neospora caninum and Toxoplasma gondii in naturally infected alpacas (Lama pacos), llamas (Lama glama) and vicunas (Lama vicugna) from Peru and Germany. Vet Parasitol. 2005;130(1-2):81-7.
Wolf, D., Schares, G., Cardenas, O., Huanca, W., Cordero, A., Bärwald, A., Conraths, F. J., Gauly, M., Zahner, H., & Bauer, C. (2005). Detection of specific antibodies to Neospora caninum and Toxoplasma gondii in naturally infected alpacas (Lama pacos), llamas (Lama glama) and vicunas (Lama vicugna) from Peru and Germany. Veterinary Parasitology, 130(1-2), 81-7.
Wolf D, et al. Detection of Specific Antibodies to Neospora Caninum and Toxoplasma Gondii in Naturally Infected Alpacas (Lama Pacos), Llamas (Lama Glama) and Vicunas (Lama Vicugna) From Peru and Germany. Vet Parasitol. 2005 Jun 10;130(1-2):81-7. PubMed PMID: 15893073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of specific antibodies to Neospora caninum and Toxoplasma gondii in naturally infected alpacas (Lama pacos), llamas (Lama glama) and vicunas (Lama vicugna) from Peru and Germany. AU - Wolf,D, AU - Schares,G, AU - Cardenas,O, AU - Huanca,W, AU - Cordero,Aida, AU - Bärwald,Andrea, AU - Conraths,F J, AU - Gauly,M, AU - Zahner,H, AU - Bauer,C, PY - 2004/11/26/received PY - 2005/03/01/revised PY - 2005/03/16/accepted PY - 2005/5/17/pubmed PY - 2005/7/27/medline PY - 2005/5/17/entrez SP - 81 EP - 7 JF - Veterinary parasitology JO - Vet Parasitol VL - 130 IS - 1-2 N2 - Sera of an experimentally Neospora caninum infected llama and a non-infected control llama were used to establish an immunoblot, an ELISA and an IFAT to detect antibodies against N. caninum tachyzoites. Subsequently, serum samples collected from a total of 871 South American Camelids (SAC: Lama glama, Lama pacos, Lama vicugna) of two farms in Peru and from 32 SAC of a farm in central Germany were examined for antibodies against N. caninum and Toxoplasma gondii. Based on the recognition of specific bands in the immunoblot, sera of SAC from Peru were differentiated into N. caninum-positive (n = 18) and T. gondii-positive (n = 30) samples and into samples negative or inconclusive for both parasites. Using the immunoblot results as the reference, a modified version of the p38-ELISA and the IFAT were evaluated for detecting N. caninum antibodies in SAC sera. Applying a cut-off as determined by two graph-receiver operating characteristic analysis both, the ELISA and the IFAT, exhibited a sensitivity and specificity of about 95% in the SAC sera from Peru. Serological testing confirmed that SAC may become infected with N. caninum under field conditions in Peru. In addition to alpacas and llamas also 114 wild living vicunas had been examined for antibodies against N. caninum. However, only the alpacas and llamas but no vicunas were found N. caninum-positive. In contrast, T. gondii-seropositive animals were detected in all three SAC species. The lack of N. caninum-seropositive vicunas indicates that in the study area in Peru wild canids might not serve as definitive hosts of N. caninum while for T. gondii a life cycle including wild felids is likely. On the German farm no N. caninum- but only T. gondii-seropositive SAC (n = 14) were detected. The seroprevalence of T. gondii infection was significantly higher in adult SAC (alpacas in Peru, llamas in Germany) than in crias (i.e. < 12 months old foals) indicating that the predominant route of infection is post natal. Since the present study was restricted to a few farms, the seroprevalences determined are not representative. However, our results confirm natural infections with N. caninum and T. gondii in SAC. Whether these infections are linked to any disease, e.g. reproductive losses, has to be clarified in further studies. SN - 0304-4017 UR - https://www.unboundmedicine.com/medline/citation/15893073/Detection_of_specific_antibodies_to_Neospora_caninum_and_Toxoplasma_gondii_in_naturally_infected_alpacas__Lama_pacos__llamas__Lama_glama__and_vicunas__Lama_vicugna__from_Peru_and_Germany_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(05)00125-1 DB - PRIME DP - Unbound Medicine ER -