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Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer.
Clin Chim Acta. 2005 Jul 24; 357(2):190-5.CC

Abstract

BACKGROUND

Kallikrein 11 (KLK11) is a newly discovered human kallikrein gene that is mainly expressed in the central nervous system and endocrine tissues. KLK11 has two alternative splicing isoforms, known as the brain type and prostate type. Many members of the human kallikrein gene family are differentially expressed in cancer and a few have potential as diagnostic/prognostic markers.

METHODS

In the present study, the expression of prostate type variant of KLK11 gene was analyzed by RT-PCR in 66 prostate cancer tissues. Tumors were pulverized, total RNA was extracted, and cDNA was prepared by reverse transcription. KLK11 was amplified by PCR using gene specific primers and its identity was verified by sequencing. Prostate tissues were then classified as KLK5 positive or negative based on eithidium bromide staining in agarose gels and image analysis.

RESULTS

KLK11 was found to be highly expressed in 43/66 (65%) of prostate cancer samples. We found a significant negative relationship between KLK11 expression and Gleason score (p = 0.004) and disease stage (p = 0.038). Serum total PSA concentration was found to be lower in patients with overexpression of KLK11 (p = 0.044).

CONCLUSIONS

We conclude that down-regulation of the KLK11 gene in advanced and more aggressive tumors may open the possibility of being used as a future biological marker distinguishing the tumor aggressiveness as well as a useful prognostic biomarker for prostate cancer.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, 157 01 Athens, Greece.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15893744

Citation

Stavropoulou, Panagiota, et al. "Expression Analysis and Prognostic Significance of Human Kallikrein 11 in Prostate Cancer." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 357, no. 2, 2005, pp. 190-5.
Stavropoulou P, Gregorakis AK, Plebani M, et al. Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer. Clin Chim Acta. 2005;357(2):190-5.
Stavropoulou, P., Gregorakis, A. K., Plebani, M., & Scorilas, A. (2005). Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer. Clinica Chimica Acta; International Journal of Clinical Chemistry, 357(2), 190-5.
Stavropoulou P, et al. Expression Analysis and Prognostic Significance of Human Kallikrein 11 in Prostate Cancer. Clin Chim Acta. 2005 Jul 24;357(2):190-5. PubMed PMID: 15893744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer. AU - Stavropoulou,Panagiota, AU - Gregorakis,Alkiviades K, AU - Plebani,Mario, AU - Scorilas,Andreas, PY - 2005/03/04/received PY - 2005/03/09/accepted PY - 2005/5/17/pubmed PY - 2005/11/3/medline PY - 2005/5/17/entrez SP - 190 EP - 5 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin. Chim. Acta VL - 357 IS - 2 N2 - BACKGROUND: Kallikrein 11 (KLK11) is a newly discovered human kallikrein gene that is mainly expressed in the central nervous system and endocrine tissues. KLK11 has two alternative splicing isoforms, known as the brain type and prostate type. Many members of the human kallikrein gene family are differentially expressed in cancer and a few have potential as diagnostic/prognostic markers. METHODS: In the present study, the expression of prostate type variant of KLK11 gene was analyzed by RT-PCR in 66 prostate cancer tissues. Tumors were pulverized, total RNA was extracted, and cDNA was prepared by reverse transcription. KLK11 was amplified by PCR using gene specific primers and its identity was verified by sequencing. Prostate tissues were then classified as KLK5 positive or negative based on eithidium bromide staining in agarose gels and image analysis. RESULTS: KLK11 was found to be highly expressed in 43/66 (65%) of prostate cancer samples. We found a significant negative relationship between KLK11 expression and Gleason score (p = 0.004) and disease stage (p = 0.038). Serum total PSA concentration was found to be lower in patients with overexpression of KLK11 (p = 0.044). CONCLUSIONS: We conclude that down-regulation of the KLK11 gene in advanced and more aggressive tumors may open the possibility of being used as a future biological marker distinguishing the tumor aggressiveness as well as a useful prognostic biomarker for prostate cancer. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/15893744/Expression_analysis_and_prognostic_significance_of_human_kallikrein_11_in_prostate_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(05)00197-X DB - PRIME DP - Unbound Medicine ER -